CAJ | 학술논문

目的了解变应性鼻炎的免疫、病理生理和形态学的改变.方法用橄榄油将甲苯-2,4-二异氰酸酯配成10%溶液作为致敏剂,滴鼻,建立豚鼠变应性鼻炎模型8例.另8例正常豚鼠作对照.致敏结束、模型成功后取血,测定红细胞C3b受体花环率和红细胞IC花环率.取鼻中隔中黏膜,采用链霉菌抗生物素蛋白-过氧化酶连结法行免疫组化染色,观察P物质在鼻黏膜中的分布.用双抗夹心ELASA方法测定血清组胺的含量.鼻黏膜做光镜和透射电镜观察.结果 (1)鼻部症状评分比较,模型组高于对照组(P＜0.01).(2)模型组的红细胞C3b受体花环率和红细胞IC花环率均低于对照组(P＜0.05).(3)模型组比对照组鼻黏膜P物质阳性细胞染色加深,阳性颗粒增多,密度增大,阳性细胞计数增多(P＜0.05).(4)组胺含量高于对照组(P＜0.01).(5)光镜下见对照组鼻黏膜上皮层为正常的假复层纤毛柱状上皮.模型组黏膜上皮增生、转化、坏死,固有层和黏膜下层改变,并见特征性的嗜酸性粒细胞、肥大细胞浸润.(6)透射电镜见对照组鼻黏膜上皮细胞结构完整,纤毛微管整齐、清晰,胞质内细胞器丰富,嗜酸性粒细胞正常.模型组黏膜上皮、细胞、纤毛、微管、微绒毛改变,细胞体积、核、细胞器改变,肥大细胞、嗜酸性粒细胞改变,血管扩张.浆液腺体旺盛增生.结论豚鼠变应性鼻炎模型在免疫、病理生理和形态学出现的改变与临床类似.
목적 료해변응성비염적면역、병리생리화형태학적개변.방법 용감람유장갑분-2,4-이이청산지배성10%용액작위치민제,적비,건립돈서변응성비염모형8례.령8례정상돈서작대조.치민결속、모형성공후취혈,측정홍세포C3b수체화배솔화홍세포IC화배솔.취비중격중점막,채용련매균항생물소단백-과양화매련결법행면역조화염색,관찰P물질재비점막중적분포.용쌍항협심ELASA방법 측정혈청조알적함량.비점막주광경화투사전경관찰.결과 (1)비부증상평분비교,모형조고우대조조(P＜0.01).(2)모형조적홍세포C3b수체화배솔화홍세포IC화배솔균저우대조조(P＜0.05).(3)모형조비대조조비점막P물질양성세포염색가심,양성과립증다,밀도증대,양성세포계수증다(P＜0.05).(4)조알함량고우대조조(P＜0.01).(5)광경하견대조조비점막상피층위정상적가복층섬모주상상피.모형조점막상피증생、전화、배사,고유층화점막하층개변,병견특정성적기산성립세포、비대세포침윤.(6)투사전경견대조조비점막상피세포결구완정,섬모미관정제、청석,포질내세포기봉부,기산성립세포정상.모형조점막상피、세포、섬모、미관、미융모개변,세포체적、핵、세포기개변,비대세포、기산성립세포개변,혈관확장.장액선체왕성증생.결론 돈서변응성비염모형재면역、병리생리화형태학출현적개변여림상유사.
Objective To understand the changes of allergic rhinitis from immunology,pathophysiology and morphology method were applied.Methods Toluene 2,4-diisocyanate was dissolved with florence oil to final concentration 10%,this solution was dropped into the nasal vestibules of animals to induce sensitization process.8 guinea pigs were prepared as allergic rhinitis with 10% Toluene 2,4-diisocyanate,and other 8 animals were used as blank controh.After model successfully established,the blood were obtained to determine RBC-C3b receptor rosette rate and RBC-imuuunocomplex rosette rate.The nasal mucosas were obtained from 2 groups,distribution and changes of substance P in nasal mucosa were observed with the application of immunohistochemical staining.The content of blood histamine was determined with ELASA method.The pathological changes of nasal mucosas were observed with the application of light microscope and transmission electron microscope.Results The behavior scores of the modeling animals were significantly higher than that of controls(P＜0.01).The value of RBC-C3b reeeptor rosette rate and RBC-imuuunoeomplex rosette rate of the modeling animals were significantly decreased than that of the controls(P＜0.05).Substance P presented in the normal nasal mucosal epithelial cells,epithelium cells of blood vessels,glandular cells and its duets,the staining density and the positive staining cells in the same aero in modeling group significantly increased,compared with controls.The counts of substance P-positive cells of control group were less than those of modeling group(P＜0.05).The content of blood histamine of the modeling animals were significantly increased compared with the controls(P＜0.01).The structure of false multiple coat cilium columnar epithelial cells in nasal mucosa of control group were successive,intact,and distinct.There were normal mucosal epithelium,lamina propria and submucosa.But modeling group showed that mucosal epithalamiums were damaged and shed,goblet cell proliferated,squamous metaphase,and epithelial necrosis happened,serous glands in lamina propria vigorously proliferated,blood vessels expanded,tissue edema formed,plenty of inflammatory cell such as eosinophil and mast cells were more in number and infiltrated.The structure of epithelial eells,cilia and mierotubule of nasal mucosa of control group were regular and distinct,there were abundant cellular organs in cytoplasm.Eosinophil cells were intact.But iu model group,mucosal epithalamium,cilia and its microtubule,mierovillus,goblet cell were damaged,the cell bulk and nucleus changed,mast cells and eosinophil cells changed,blood vessels expanded,serous glands vigorously proliferated.This morphological change was roughly identical to clinical manifestation of allergic rhinitis.Conclusion Toluene-2,4-diisocyanate can be used to establish allergic rhinitis model in guinea pigs,and some ehanges of the allergic rhinitis in model guinea pig were similar with clinic observation.