CAJ | 학술논문

目的探讨结合珠蛋白(Hp)基因多态性与急性冠脉综合征(ACS)易感性的关系.方法采用病例对照研究,选择2005年3月至2006年12月浙江大学医学院附属第二医院心内科住院并行冠脉造影检查证实的ACS患者112例(包括急性心肌梗死57例、不稳定性心绞痛55例)和同期年龄、性别匹配的健康体检者124例.采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测所有受试者Hp基因型,探讨Hp基因多态性与ACS发生的关系.用Hardy-Weinberg平衡检验样本群体代表性;各组基因型和等位基因频率差异比较采用x2检验,并以比值比(OR)及其95%可信区间(95%CI)表示相对的风险度;综合评价ACS危险性采用多因素Logistic回归分析;以P＜0.05为差异具有统计学意义.结果 ACS组Hp基因型分布与对照组相比差异具有统计学意义(P=0.003),表现为ACS组的Hp2-2基因型频率明显高于对照组(0.571 vs.0.355,P=0.001;OR=2.419,95%CI1.427～4.100),Hp2等位基因的频率也明显高于对照组(0.759 vs.0.616,P=0.001;OR=1.965,95%CI1.316～2.934).同时,多因素Logistic回归分析表明Hp2-2基因型是ACS的独立危险因素(P=0.002;OR=2.557,95%CI:1.410～4.639).结论 Hp2-2基因型与ACS的发生密切相关,可能是ACS发病的独立危险因子;Hp2等位基因可能是ACS的遗传易感基因.
목적 탐토결합주단백(Hp)기인다태성여급성관맥종합정(ACS)역감성적관계.방법 채용병례대조연구,선택2005년3월지2006년12월절강대학의학원부속제이의원심내과주원병행관맥조영검사증실적ACS환자112례(포괄급성심기경사57례、불은정성심교통55례)화동기년령、성별필배적건강체검자124례.채용취합매련반응-한제성편단장도다태성(PCR-RFLP)기술검측소유수시자Hp기인형,탐토Hp기인다태성여ACS발생적관계.용Hardy-Weinberg평형검험양본군체대표성;각조기인형화등위기인빈솔차이비교채용x2검험,병이비치비(OR)급기95%가신구간(95%CI)표시상대적풍험도;종합평개ACS위험성채용다인소Logistic회귀분석;이P＜0.05위차이구유통계학의의.결과 ACS조Hp기인형분포여대조조상비차이구유통계학의의(P=0.003),표현위ACS조적Hp2-2기인형빈솔명현고우대조조(0.571 vs.0.355,P=0.001;OR=2.419,95%CI1.427～4.100),Hp2등위기인적빈솔야명현고우대조조(0.759 vs.0.616,P=0.001;OR=1.965,95%CI1.316～2.934).동시,다인소Logistic회귀분석표명Hp2-2기인형시ACS적독립위험인소(P=0.002;OR=2.557,95%CI:1.410～4.639).결론 Hp2-2기인형여ACS적발생밀절상관,가능시ACS발병적독립위험인자;Hp2등위기인가능시ACS적유전역감기인.
Objective To assess the association of Haptoglobin(Hp) polymorphism with acute coronary syndrome(ACS) in Chinese. Method A total of 112 patients with ACS including 57 patients with acute myocardial infarction and 55 patients with unstable angina pectoris confirmed with angiography and 121healthy controls were recruited in this study. Polymerase chain reaction (PCR) method was utilized to genotype Hpl and Hp2 alleles and genotype frequencies in cases and controls were compared. All polymorphisms were test of Hardy-Weinberg equilibrium in both groups separately. The differences of genotypes and alleles between two groups were analyzed with x2 test. The association between Hp polymorphism and the risk of ACS was estimated by odds ratio (OR) and their 95% confidence intervals (95% CI), and the comprehensive evaluation of the factors associated with ACS were determined by using multivariate logistic regression analysis. P ＜0.05 was considered to be statistically significant. Results The frequency of Hp2-2 genotype was significantly higher in ACSs than in controls (0. 571 vs. 0. 355, P = 0. 001; OR = 2. 419, 95% CI:1. 427 ～4. 100), multivariate Logistic regression analysis indicates that Hp2-2 genotype is an independent risk factor to ACS (P = 0.002; OR = 2.557,95% CI: 1. 392 - 4.637). Similarly, the Hp2 allele frequency in ACS groups was significantly higher than that in the control subjects (0. 759 vs. 0. 616, P =0.001; OR = 1. 965,95% CI 1. 316 ～2. 934). Conclusion The Hp2-2 genotype is associated with ACS in Chinese. Hp2-2 genotype may be an independent risk factor to ACS, and Hp2 allele may be a genetic susceptibility factor to ACS in Chinese.