CAJ | 학술논문

目的探讨重组弓形虫膜表面抗原SAG1基因的表达产物-原核表达蛋白(rSAG1)用于弓形虫病的免疫诊断价值.方法用异丙基-B-D-硫代吡喃半乳糖苷(IPTG)诱导大肠埃希菌重组质粒pET28a-SAG1(pET28a-SAG1/BL21)表达,纯化重组pET28a-SAG1/BL21弓形虫膜表面抗原SAG1基因表达产物;用弓形虫缓殖子感染的鼠血清、正常鼠血清和10例弓形虫患者血清为一抗,基因表达产物rSAG1用免疫印迹法进行鉴定,比较rSAG1在弓形虫病免疫诊断中的价值.结果纯化重组弓形虫膜表面抗原SAG1基因后获得了相对分子质量约38.5×103的表达产物rSAG1;表面抗原SAG1可被弓形虫缓殖子感染的鼠血清所识别;10例弓形虫患者血清在免疫印迹诊断中,有4例出现了弓形虫膜表面抗原SAG1基因表达产物rSAG1.结论 rSAG1具备一定的弓形虫病的免疫诊断价值.
목적 탐토중조궁형충막표면항원SAG1기인적표체산물-원핵표체단백(rSAG1)용우궁형충병적면역진단개치.방법 용이병기-B-D-류대필남반유당감(IPTG)유도대장애희균중조질립pET28a-SAG1(pET28a-SAG1/BL21)표체,순화중조pET28a-SAG1/BL21궁형충막표면항원SAG1기인표체산물;용궁형충완식자감염적서혈청、정상서혈청화10례궁형충환자혈청위일항,기인표체산물rSAG1용면역인적법진행감정,비교rSAG1재궁형충병면역진단중적개치.결과 순화중조궁형충막표면항원SAG1기인후획득료상대분자질량약38.5×103적표체산물rSAG1;표면항원SAG1가피궁형충완식자감염적서혈청소식별;10례궁형충환자혈청재면역인적진단중,유4례출현료궁형충막표면항원SAG1기인표체산물rSAG1.결론 rSAG1구비일정적궁형충병적면역진단개치.
Objective To investigate the diagnostic value of the recombinant surface antigen 1 (rSAG1) in immunodiagnosis of toxoplasmosis. Methods Isopropyl β-D- 1 -thio-galaetopyranoside (IPTG) was used to induce the expression of recombinant plasmid pET28a-SAG1 of Escherich coli(pET28a-SAG1/BL21 ). The expression products (rSAG1) of pET28a-SAG1/BL21 were identified by Western blotting. The serum of mice infected with Toxoplasma gondii tachyzoites, normal mouse serum and the serum from 10 toxoplasma gondii patients were used as primary anti-Toxoplasma gondii antibodies, and the rSAG1 gene products were identified by Western blotting, by which the diagnostic value of rSAG1 in Toxoplasmosis was compared. Results After induction and purification, rSAG1 protein was obtained and its relative molecular mass was 38.5 × 103. The fusion protein could be recognized by the serum of mouse infected with Toxoplasma gondii tachyzoites, rSAG1 of expression products of surface membrane antigen SAG1 gene from Toxoplasma Gondii could be detected in 4 cases from 10 patients by Westem blotting.Conclusion The rSAG1 has a potential value in the immunodiagnosis of Toxoplasmosis.