CAJ | 학술논문

目的通过电刺激正常及癫痫大鼠丘脑底核(STN),研究黑质网状部(SNr)及苍白球(GP)细胞外液中谷氨酸(Glu)、γ-氨基丁酸(GABA)的变化,探讨电刺激治疗癫痫的机制.方法正常大鼠和癫痫大鼠各加只,将刺激电极植入一侧STN,分别用130 Hz和260 Hz进行刺激,同时在同侧的SNr和GP收集细胞外液,用高压液相色谱法检测其Glu和GABA的含量.结果癫痫大鼠SNr的GABA基础值明显高于正常大鼠.电刺激使两组SNr的GABA明显升高.130 Hz和260 Hz刺激明显增高两组GP和SNr的Glu含量,但130 Hz的更显著.结论 SNr细胞外GABA升高在STN电刺激治疗中起重要作用.电刺激增加了GP细胞的活动,STN电刺激治疗癫痫机制不能单纯解释为"功能的毁损".
목적 통과전자격정상급전간대서구뇌저핵(STN),연구흑질망상부(SNr)급창백구(GP)세포외액중곡안산(Glu)、γ-안기정산(GABA)적변화,탐토전자격치료전간적궤제.방법 정상대서화전간대서각가지,장자격전겁식입일측STN,분별용130 Hz화260 Hz진행자격,동시재동측적SNr화GP수집세포외액,용고압액상색보법검측기Glu화GABA적함량.결과 전간대서SNr적GABA기출치명현고우정상대서.전자격사량조SNr적GABA명현승고.130 Hz화260 Hz자격명현증고량조GP화SNr적Glu함량,단130 Hz적경현저.결론 SNr세포외GABA승고재STN전자격치료중기중요작용.전자격증가료GP세포적활동,STN전자격치료전간궤제불능단순해석위"공능적훼손".
Objective To study the change of extracellular glutamate(Glu)and γ-aminobutyric acid(GABA)in substantia nigra(SNr)and globus pallidus(GP)during high frequency stimulation of subthalamic nucleus(STN)in normal and epileptic rats.Methods Two animal groups:20 epilepsy model rats and 20 normal rats were used.Concentric bipolar electrodes were stereotaxically implanted in the unilateral STN,stimulated in high frequency 130 Hz,260 Hz in each group.The microdialysis probes were unilaterally lowered into the ipsilateral GP and SNr.The concentrations of Glu and GABA in dialysate samples Were determined by high-performance liquid chromatography(HPLC).Results There was significant increase in the basal level GABA in SNr of epileptic rats.In both groups.electrical stimulation of STN induced increases of GABA contents in SNr.In addition.the frequency of 130 Hz provoked the maximal increase of Glu contents both in GP and SNr.whereas 260 Hz induced less effect.Conclusions The increase of GA BA during electrical STN stimulation plays an important role in acting against the seizure.The GP activity is increased by STN stimulation.The impact of STN HFS should not be considered as a simple"functionai lesion"of STN.