中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2009年
2期
121-123
,共3页
朱玉方%庞琦%栾立明%贺红卫%许尚臣%赵光宇%葛明旭%王汉斌%张康%孔大伟%刘伟
硃玉方%龐琦%欒立明%賀紅衛%許尚臣%趙光宇%葛明旭%王漢斌%張康%孔大偉%劉偉
주옥방%방기%란립명%하홍위%허상신%조광우%갈명욱%왕한빈%장강%공대위%류위
颅内动脉瘤%Ⅲ型胶原蛋白α1链基因%单核苷酸多态性
顱內動脈瘤%Ⅲ型膠原蛋白α1鏈基因%單覈苷痠多態性
로내동맥류%Ⅲ형효원단백α1련기인%단핵감산다태성
Intracranial aneurysm%COL3A1%Single nucleotide polymorphism
目的 在颅内动脉瘤组和对照组中检测与分析Ⅲ型胶原蛋白α1链(Type Ⅲ collagen alpha1,COL3A1)基因多态性.方法 利用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法 比较243例颅内动脉瘤与260例正常对照之间COL3A1基因第30外显子+2209 A/G多态性的基因型及等位基因频率的构成.结果 颅内动脉瘤组与对照组中GA+AA基因型频率差异有统计学意义,前者(41.56%)明显高于后者(28.08%)(χ2=10.15,P=0.001),动脉瘤组A等位基因频率为23.66%,对照组为18.46%,二者的差异有统计学意义(χ2=9.13,P=0.003),用logistic回归纠正年龄、性别、高血压、吸烟及饮酒等因素结果 仍有意义(χ2=6.76,P=0.009).结论 颅内囊性动脉瘤和对照组之间COL3A1基因第30外显子+2209A/G多态性差异有统计学意义.
目的 在顱內動脈瘤組和對照組中檢測與分析Ⅲ型膠原蛋白α1鏈(Type Ⅲ collagen alpha1,COL3A1)基因多態性.方法 利用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)方法 比較243例顱內動脈瘤與260例正常對照之間COL3A1基因第30外顯子+2209 A/G多態性的基因型及等位基因頻率的構成.結果 顱內動脈瘤組與對照組中GA+AA基因型頻率差異有統計學意義,前者(41.56%)明顯高于後者(28.08%)(χ2=10.15,P=0.001),動脈瘤組A等位基因頻率為23.66%,對照組為18.46%,二者的差異有統計學意義(χ2=9.13,P=0.003),用logistic迴歸糾正年齡、性彆、高血壓、吸煙及飲酒等因素結果 仍有意義(χ2=6.76,P=0.009).結論 顱內囊性動脈瘤和對照組之間COL3A1基因第30外顯子+2209A/G多態性差異有統計學意義.
목적 재로내동맥류조화대조조중검측여분석Ⅲ형효원단백α1련(Type Ⅲ collagen alpha1,COL3A1)기인다태성.방법 이용취합매련반응-한제성편단장도다태성(PCR-RFLP)방법 비교243례로내동맥류여260례정상대조지간COL3A1기인제30외현자+2209 A/G다태성적기인형급등위기인빈솔적구성.결과 로내동맥류조여대조조중GA+AA기인형빈솔차이유통계학의의,전자(41.56%)명현고우후자(28.08%)(χ2=10.15,P=0.001),동맥류조A등위기인빈솔위23.66%,대조조위18.46%,이자적차이유통계학의의(χ2=9.13,P=0.003),용logistic회귀규정년령、성별、고혈압、흡연급음주등인소결과 잉유의의(χ2=6.76,P=0.009).결론 로내낭성동맥류화대조조지간COL3A1기인제30외현자+2209A/G다태성차이유통계학의의.
Objective To test and analyze the polymorphism of the Type Ⅲ Collagen alpha1 gene in intracranial aneurysms. Methods To compare the genotype frequency and A allele frequency of the COL3A1 + 2209A/G polymorphism in 243 patients with intracranial aneurysms and 260 controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results The GA + AA genotype frequency of ancurysm group (41.56%) was much higher than that of the control group (28.08%) (χ2=10.15, P=0.001). The A allele frequency of aneurysm group (23.66%) was also higher than that of the control group (18.46 %) (χ2=9.13, P=0.003). Adjustment for age, sex, hypertension, smoking and alcohol consumption using logistic regression did not change the conclusions(χ2 = 6.76, P = 0. 009). Conclusions There is significant of difference of COL3A1 + 2209A/G polymorphism between intracranial aneurysms and controls.
