中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2011年
10期
890-894
,共5页
朱兵清%任红宇%周海健%秦天%邵祝军
硃兵清%任紅宇%週海健%秦天%邵祝軍
주병청%임홍우%주해건%진천%소축군
军团病杆菌,嗜肺%细菌分型技术%电泳,凝胶,脉冲场
軍糰病桿菌,嗜肺%細菌分型技術%電泳,凝膠,脈遲場
군단병간균,기폐%세균분형기술%전영,응효,맥충장
Legionella pneumophila%Bacterial typing techniques%Electrophoresis,gel,pulsedfield
目的 了解我国环境水中嗜肺军团菌血清1型(Lp1)菌株的序列分型特征,并初步建立我国军团菌序列分型数据库。方法 采用序列分型(SBT)方法,对我国9个省(市、自治区)2005-2008年间环境水中分离的82株Lp1菌株进行分型,同时采用脉冲场凝胶电泳(PFGE)分型方法对这些菌株进行分型,并采用BioNumerics 5.1软件对2种方法的分型结果进行聚类分析和比较。结果 82株Lp1菌株分为22种序列(ST)型,其中17种ST型是新序列型,新发现1个等位基因;ST-1型为主要的序列型,在8个省(市、自治区)均有发现,该型菌株占所有菌株的46.3% (38/82);ST-1、ST-150、ST-154、ST-159、ST-160和ST-630出现于2个以上的分离地点,5个分离位点(B4、B5、B6、S3和S8)发现2种以上ST型;通过聚类分析,15种ST型被分为3个序列群(ST-1序列群、ST-154序列群和ST-149序列群),其余7种ST型没有序列群归类。采用PFGE分型方法,这些菌株可被分为46种带型。SBT和PFGE两种方法结合可将82株菌株分为54种分子型别。两种方法的聚类分析结果具有良好的一致性。结论 我国环境水中Lp1菌株具有独特的SBT分布;通过研究,初步建立了我国军团菌序列分型数据库。
目的 瞭解我國環境水中嗜肺軍糰菌血清1型(Lp1)菌株的序列分型特徵,併初步建立我國軍糰菌序列分型數據庫。方法 採用序列分型(SBT)方法,對我國9箇省(市、自治區)2005-2008年間環境水中分離的82株Lp1菌株進行分型,同時採用脈遲場凝膠電泳(PFGE)分型方法對這些菌株進行分型,併採用BioNumerics 5.1軟件對2種方法的分型結果進行聚類分析和比較。結果 82株Lp1菌株分為22種序列(ST)型,其中17種ST型是新序列型,新髮現1箇等位基因;ST-1型為主要的序列型,在8箇省(市、自治區)均有髮現,該型菌株佔所有菌株的46.3% (38/82);ST-1、ST-150、ST-154、ST-159、ST-160和ST-630齣現于2箇以上的分離地點,5箇分離位點(B4、B5、B6、S3和S8)髮現2種以上ST型;通過聚類分析,15種ST型被分為3箇序列群(ST-1序列群、ST-154序列群和ST-149序列群),其餘7種ST型沒有序列群歸類。採用PFGE分型方法,這些菌株可被分為46種帶型。SBT和PFGE兩種方法結閤可將82株菌株分為54種分子型彆。兩種方法的聚類分析結果具有良好的一緻性。結論 我國環境水中Lp1菌株具有獨特的SBT分佈;通過研究,初步建立瞭我國軍糰菌序列分型數據庫。
목적 료해아국배경수중기폐군단균혈청1형(Lp1)균주적서렬분형특정,병초보건립아국군단균서렬분형수거고。방법 채용서렬분형(SBT)방법,대아국9개성(시、자치구)2005-2008년간배경수중분리적82주Lp1균주진행분형,동시채용맥충장응효전영(PFGE)분형방법대저사균주진행분형,병채용BioNumerics 5.1연건대2충방법적분형결과진행취류분석화비교。결과 82주Lp1균주분위22충서렬(ST)형,기중17충ST형시신서렬형,신발현1개등위기인;ST-1형위주요적서렬형,재8개성(시、자치구)균유발현,해형균주점소유균주적46.3% (38/82);ST-1、ST-150、ST-154、ST-159、ST-160화ST-630출현우2개이상적분리지점,5개분리위점(B4、B5、B6、S3화S8)발현2충이상ST형;통과취류분석,15충ST형피분위3개서렬군(ST-1서렬군、ST-154서렬군화ST-149서렬군),기여7충ST형몰유서렬군귀류。채용PFGE분형방법,저사균주가피분위46충대형。SBT화PFGE량충방법결합가장82주균주분위54충분자형별。량충방법적취류분석결과구유량호적일치성。결론 아국배경수중Lp1균주구유독특적SBT분포;통과연구,초보건립료아국군단균서렬분형수거고。
Objective To analyze the characteristics of Sequence-based Typing (SBT) of the Serotype 1 Legionella pneumophila ( Lp1 ) isolated from environmental water in China, and then create a preliminary database. Methods A total of 82 strains of Lp1 isolated from environmental water in 9 provinces of China between 2005 and 2008 were genotyped by SBT method and Pulsed-field Gel Electrophoresis (PFGE) method. The results of the two different typing methods were then compared by cluster analysis,adopting BioNumerics version 5.1 software. Results By SBT method, the 82 strains of Lpl were divided into 22 ST types,of which 17 new types and one new allele was discovered. The dominant type was ST-1 type,found in 8 provinces, accounting for 46. 3% (38/82). ST-1, ST-150, ST-154, ST-159, ST-160 and ST-630 types were found in more than 2 isolated-sites; while more than 2 different ST types were found in 5 isolated-sites,as site B4, BS, B6, S3 and S8. In cluster analysis, 15 ST types were grouped into three complexes (ST-1 complex, ST-154 complex and ST-149 complex); and the other 7 ST types were not assigned complex. By PFGE method,46 banding patterns were observed. As a result of the combination of the two methods, the 82 isolates strains could be divided into 54 molecular types, which showed a reliable accordance in the cluster analysis between the two methods. ConclusionThe SBT of the Lp1 in environmental water in China was unique. From the study, a preliminary SBT database was set up.