中华心律失常学杂志
中華心律失常學雜誌
중화심률실상학잡지
CHINESE JOURNAL OF CARDIAC ARRHYTHMIAS
2010年
3期
224-227
,共4页
沈文志%王德国%张凤祥%翟立上%曹明勇%居维竹%陈红武%杨兵%陈明龙%曹克将
瀋文誌%王德國%張鳳祥%翟立上%曹明勇%居維竹%陳紅武%楊兵%陳明龍%曹剋將
침문지%왕덕국%장봉상%적립상%조명용%거유죽%진홍무%양병%진명룡%조극장
骨髓间充质干细胞%心肌梗死%细胞移植%钾离子通道
骨髓間充質榦細胞%心肌梗死%細胞移植%鉀離子通道
골수간충질간세포%심기경사%세포이식%갑리자통도
Bone marrow mesenchymal stem cells%Myocardial infarction%Cell transplantation%Transient outward potassium subunit Kv4. 2
目的 研究心肌梗死后移植骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMMSCs)对心室肌细胞钾离子通道Ito亚单位Kv4.2基因表达的影响方法 40只SD(Sprague-Dawley)大鼠随机分成4组(10只/组):假手术组、心肌梗死组、心肌梗死+干细胞组和心肌梗死+细胞培养基组.开胸结扎冠状动脉前降支建立心肌梗死模型,建模成功后,在梗死周围分别注入BMMSCs和细胞培养基,心肌梗死组仅建立心肌梗死模型,假手术组仅开胸子以前降支穿线但不结扎.2周后行心肌组织HE染色和荧光显微镜观察移植细胞,逆转录聚合酶链反应(RT-PCR)和Western blot分别检测钾离子通道Ito亚单位Kv4.2基因mRNA和蛋白水平.结果 (1)心肌组织免疫荧光检测发现,BMMSCs集中分布于梗死区和梗死心肌周围;(2)心肌梗死组和心肌梗死+细胞培养基组Kv4.2 mRNA量(0.39±0.02,0.41±0.04)和蛋白量(0.47±0.02,0.50±0.05)明显下降,与假手术组(0.76±0.05,0.74±0.06)相比差异有统计学意义(P＜0.01);心肌梗死+干细胞组Kv4.2 mRNA量和蛋白表达量(0.57±0.05,0.64±0.03)较心肌梗死组(0.39±0.02,0.47±0.02)明显升高(P＜0.01).结论 骨髓间充质干细胞移植后心肌梗死大鼠钾离子通道Ito亚单位Kv4.2基因表达上升,可能减少心律失常发生.
目的 研究心肌梗死後移植骨髓間充質榦細胞(bone marrow mesenchymal stem cells,BMMSCs)對心室肌細胞鉀離子通道Ito亞單位Kv4.2基因錶達的影響方法 40隻SD(Sprague-Dawley)大鼠隨機分成4組(10隻/組):假手術組、心肌梗死組、心肌梗死+榦細胞組和心肌梗死+細胞培養基組.開胸結扎冠狀動脈前降支建立心肌梗死模型,建模成功後,在梗死週圍分彆註入BMMSCs和細胞培養基,心肌梗死組僅建立心肌梗死模型,假手術組僅開胸子以前降支穿線但不結扎.2週後行心肌組織HE染色和熒光顯微鏡觀察移植細胞,逆轉錄聚閤酶鏈反應(RT-PCR)和Western blot分彆檢測鉀離子通道Ito亞單位Kv4.2基因mRNA和蛋白水平.結果 (1)心肌組織免疫熒光檢測髮現,BMMSCs集中分佈于梗死區和梗死心肌週圍;(2)心肌梗死組和心肌梗死+細胞培養基組Kv4.2 mRNA量(0.39±0.02,0.41±0.04)和蛋白量(0.47±0.02,0.50±0.05)明顯下降,與假手術組(0.76±0.05,0.74±0.06)相比差異有統計學意義(P＜0.01);心肌梗死+榦細胞組Kv4.2 mRNA量和蛋白錶達量(0.57±0.05,0.64±0.03)較心肌梗死組(0.39±0.02,0.47±0.02)明顯升高(P＜0.01).結論 骨髓間充質榦細胞移植後心肌梗死大鼠鉀離子通道Ito亞單位Kv4.2基因錶達上升,可能減少心律失常髮生.
목적 연구심기경사후이식골수간충질간세포(bone marrow mesenchymal stem cells,BMMSCs)대심실기세포갑리자통도Ito아단위Kv4.2기인표체적영향방법 40지SD(Sprague-Dawley)대서수궤분성4조(10지/조):가수술조、심기경사조、심기경사+간세포조화심기경사+세포배양기조.개흉결찰관상동맥전강지건립심기경사모형,건모성공후,재경사주위분별주입BMMSCs화세포배양기,심기경사조부건립심기경사모형,가수술조부개흉자이전강지천선단불결찰.2주후행심기조직HE염색화형광현미경관찰이식세포,역전록취합매련반응(RT-PCR)화Western blot분별검측갑리자통도Ito아단위Kv4.2기인mRNA화단백수평.결과 (1)심기조직면역형광검측발현,BMMSCs집중분포우경사구화경사심기주위;(2)심기경사조화심기경사+세포배양기조Kv4.2 mRNA량(0.39±0.02,0.41±0.04)화단백량(0.47±0.02,0.50±0.05)명현하강,여가수술조(0.76±0.05,0.74±0.06)상비차이유통계학의의(P＜0.01);심기경사+간세포조Kv4.2 mRNA량화단백표체량(0.57±0.05,0.64±0.03)교심기경사조(0.39±0.02,0.47±0.02)명현승고(P＜0.01).결론 골수간충질간세포이식후심기경사대서갑리자통도Ito아단위Kv4.2기인표체상승,가능감소심률실상발생.
Objective To investigate the effects of bone marrow mesenchymal stem cells (BMMSCs) implantation on transient outward potassium Kv4. 2 of cardiomyocytes in postinfarcted rat left ventricle myocardium. Methods Total 40 Sprague-Dawley(SD) rats were divided into four groups at random:sham-operating group,myocardial infarction (MI) group, BMMSCs transplantation (MI-BMMSCs) group and cell medium(MI-CM) group,10 rats in the each group. MI was induced by ligation of left anterior descending artery. About 1h after the ligation BMMSCs cells suspension labeled by DAPI,were injected into different acute myocardial ischemia regions. While,in MI-CM group,DMEM medium was injected into similar regions as the MI-BMMSCs group. The rats were sacrificed at the end of the second week. Left ventricular histopathologic myocardial changes were evaluated through H&E. Transplanted cells were observed by fluorescent microscope. RT-PCR and Western blot were used to identify expression of Ito Kv4. 2. Results( 1 ) Fluorescent microscope showed that MSC-derived cells survived. (2) The expression of Kv4. 2 in the MI group and MI-CM group was significantly lower than that in the sham-operating group (P ＜0. 01 ). Compared to MI-CM group and MI group, the expression of Kv4. 2 was significantly increased( P ＜0. 01 ). Conclusion The MSCs transplantation treatment can promote the expression of Kv4. 2 in AMI rats,which may contribute to decrease of arrhythmias after MSCs transplantation.
