CAJ | 학술논문

目的观察糖尿病视网膜病变(DR)患者外周血内皮祖细胞(EPC)数量及功能变化.方法选取DR患者12例(DR组)、糖尿病(DM)患者18例(DM组)及无DM的老年性白内障患者15例(对照组)纳入研究.密度梯度离心法收集各组患者外周血单个核细胞,培养后第10天采用流式细胞仪计数EPC细胞阳性率.采用二苯基四氮唑嗅盐(MTT)比色法、黏附能力测定实验及Transwell实验检测EPC的增生、黏附和迁移能力.结果流式细胞仪检测结果显示,对照组、DM组及DR组培养后第10天EPC细胞阳性率分别为(45.190±1.287)％、(30.130±3.245)％、(37.370±2.501)％；3组间差异有统计学意义(F=27.690,P=0.001).MTT比色法检测结果显示,对照组、DM组及DR组吸光度[A,旧称光密度(OD)]值分别为0.330±0.047、0.225±0.042、0.120±0.029；3组间差异有统计学意义(F=29.327,P=0.000).黏附能力测定实验结果显示,对照组、DM组及DR组EPC细胞数分别为76.400±7.503、51.167±6.646、26.500±7.853；3组间差异有统计学意义(F=56.612,P=0.000).Transwell实验结果显示,对照组、DM组及DR组EPC细胞数分别为23.600±6.504、20.833±4.491、12.000±2.944;3组间差异有统计学意义(F=6.477,P=0.012).结论 DR患者外周血EPC数量减少,且其增生、黏附和迁移能力受损.
목적 관찰당뇨병시망막병변(DR)환자외주혈내피조세포(EPC)수량급공능변화.방법 선취DR환자12례(DR조)、당뇨병(DM)환자18례(DM조)급무DM적노년성백내장환자15례(대조조)납입연구.밀도제도리심법수집각조환자외주혈단개핵세포,배양후제10천채용류식세포의계수EPC세포양성솔.채용이분기사담서후염(MTT)비색법、점부능력측정실험급Transwell실험검측EPC적증생、점부화천이능력.결과 류식세포의검측결과현시,대조조、DM조급DR조배양후제10천EPC세포양성솔분별위(45.190±1.287)％、(30.130±3.245)％、(37.370±2.501)％；3조간차이유통계학의의(F=27.690,P=0.001).MTT비색법검측결과현시,대조조、DM조급DR조흡광도[A,구칭광밀도(OD)]치분별위0.330±0.047、0.225±0.042、0.120±0.029；3조간차이유통계학의의(F=29.327,P=0.000).점부능력측정실험결과현시,대조조、DM조급DR조EPC세포수분별위76.400±7.503、51.167±6.646、26.500±7.853；3조간차이유통계학의의(F=56.612,P=0.000).Transwell실험결과현시,대조조、DM조급DR조EPC세포수분별위23.600±6.504、20.833±4.491、12.000±2.944;3조간차이유통계학의의(F=6.477,P=0.012).결론 DR환자외주혈EPC수량감소,차기증생、점부화천이능력수손.
Objective To observe the changes in the number and activity of endothelial progenitor cell (EPC) from peripheral blood in patients with diabetic retinopathy (DR).Methods Twelve patients with DR (DR group),18 patients with diabetic mellikus (DM) without DR (DM group),and 15 patients with age-related cataract without DM (control group) were enrolled in this study. Peripheral blood mononuclear cells were isolated by density gradient centrifugation.After 10 days culture,positive rate of EPC was counted by flow cytometry,proliferation,adhesion and migration activities were assayed by MTT chromatometry,adhesion activity assay essay and Transwell assay.Results Flow cytometric rest showed that positive rate of EPC in patients of DR group,DM group and control group was (37.370±2.501)％,(30.130±3.245)％ and (45.190±1.287)％.There was a significant difference between these three groups (F=27.690,P=0.001).MTT chromatometry showed that A-values of DR group,DM group and control group were 0.330±0.047,0.225±0.042 and 0.120±0.029.There was a significant difference between these three groups(F=29.327,P=0.000).Adhesion activity assay essay showed that EPC numbers in DR group,DM group and control group were 76.400±7.503,51.167±6.646 and 26.500±7.853.There was a significant difference between these three groups (F=56.612,P=0.000).Transwcll assay showed that EPC numbers in DR group,DM group and control group were 23.600±6.504,20.833±4.491 and 12.000±2.944.There was a significant difference between these three groups (F=6.477,P=0.012).Conclusion EPC from peripheral blood in patients with DR exhibit reduced numbers and impaired proliferation,adhesion and migration activity.