CAJ | 학술논문

目的探讨整合素-α2基因(integrin alpha-2,ITGA2)C807T和整合索-β3基因(integrinbeta-3,ITGB3)T176C多态性与缺血性脑卒中的关系及其对血脂、脂蛋白水平的影响.方法应用聚合酶链反应-限制性片段长度多态性和DNA测序的方法检测265例缺血性脑卒中患者和280名对照组ITGA2和ITGB3的基因型;同时按常规方法测定血浆脂质、脂蛋白水平.结果缺血性脑卒中组总胆固醇(totalcholesterol,TC),甘油三酯(triacylglycerol,TG)、低密度脂蛋白-胆固醇(low density lipoprotein-cholesterol,LDL-C)水平明显高于对照组(P<0.05),ITGB3基因T176C多态性在缺血性脑卒中组和正常人群中的分布差异无统计学意义(P>0.05).而ITGA2基因C807T多态性在两组人群中的分布差异有统计学意义(P<0.05),等位基因频率的相对风险分析发现,T等位基因携带者患缺血性脑卒中的风险是C等位基因的1.455倍(OR=1.455,95%CI:1.134～1.866),携带T等位基因的缺血性脑卒中个体血浆TC水平显著高于不携带者(P<0.05).结论 ITGA2基因C807T多态性与缺血性脑卒中的发病具有相关性,其中T等位基因可能是缺血性脑卒中的遗传易感基因;ITGA2基因C807T多态性可能通过影响血脂水平而影响缺血性脑卒中的发生.
목적 탐토정합소-α2기인(integrin alpha-2,ITGA2)C807T화정합색-β3기인(integrinbeta-3,ITGB3)T176C다태성여결혈성뇌졸중적관계급기대혈지、지단백수평적영향.방법 응용취합매련반응-한제성편단장도다태성화DNA측서적방법검측265례결혈성뇌졸중환자화280명대조조ITGA2화ITGB3적기인형;동시안상규방법측정혈장지질、지단백수평.결과 결혈성뇌졸중조총담고순(totalcholesterol,TC),감유삼지(triacylglycerol,TG)、저밀도지단백-담고순(low density lipoprotein-cholesterol,LDL-C)수평명현고우대조조(P<0.05),ITGB3기인T176C다태성재결혈성뇌졸중조화정상인군중적분포차이무통계학의의(P>0.05).이ITGA2기인C807T다태성재량조인군중적분포차이유통계학의의(P<0.05),등위기인빈솔적상대풍험분석발현,T등위기인휴대자환결혈성뇌졸중적풍험시C등위기인적1.455배(OR=1.455,95%CI:1.134～1.866),휴대T등위기인적결혈성뇌졸중개체혈장TC수평현저고우불휴대자(P<0.05).결론 ITGA2기인C807T다태성여결혈성뇌졸중적발병구유상관성,기중T등위기인가능시결혈성뇌졸중적유전역감기인;ITGA2기인C807T다태성가능통과영향혈지수평이영향결혈성뇌졸중적발생.
Objective To study the association of integrin alpha-2 (ITGA2) gene C807T, integrin beta-3(ITGB3) gene T176C polymorphisms with ischemic stroke and the effect of the polymorphisms on plasma lipid and lipoprotein levels. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing were used to detect the integrin genotypes in 265 patients with iscbemic stroke and 280 healthy controls. The plasma lipid and lipoprotein levels were measured by routine method. Results Plasma total cholesterol (TC), triacylglycerol (TG) and low density lipoprotein-cholesterol (LDL-C) in the patients with ischemic stroke were significantly higher than those in the controls (P<0.05). The distributions of the ITGB3 gene T176C polymorphism were not different between the ischemie stroke group and control group, but the ITGA2 gene C807T polymorphism was significantly different. The relative risk suffering from iscbemic stroke of the T allele carrier was 1.455 times as that of the C allele carrier (OR=1.455,95 % CI:1.134-1.866). The level of plasma lipid in the T allele carriers was significantly higher than that in the C allele carriers (P<0.05). Conclusion The ITGA2 gene C807T polymorphism was associated with ischemic stroke, the 807 T allele may be a genetic risk factor for ischemic stroke. The ITGA2 gene C807T polymorphism may affect ischemic stroke through plasma lipid and lipoprotein levels.