中国化学工程学报(英文版)
中國化學工程學報(英文版)
중국화학공정학보(영문판)
CHINESE JOURNAL OF CHEMICAL ENGINEERING
2007年
5期
725-729
,共5页
王天奇%李翰祥%王满意%谭天伟
王天奇%李翰祥%王滿意%譚天偉
왕천기%리한상%왕만의%담천위
ergosterol%(1→3)-α-D-glucan%chitosan%extraction%Penicillium chrysogenum
Ergosterol,(1→3)-α-D-glucan and chitosan are important biomaterials. In this research, a process has been developed to integratively extract ergosterol, (1→3)-α-D-glucan, and chitosan from Penicillium chrysongenum mycelium. First, the mycelia are pretreated with 0.1mol·L-1 of NaOH. After recovery by centrifugation, the solid
portion is made to undergo saponification and deacetylation reactions by addition of 2mol·L-1 NaOH and ethanol.
After reaction, extraction is carried out by addition of petroleum ether, which separates the reaction mixture into two phases. The upper layer of petroleum ether contains extracted ergosterol, and the bottom layer of NaOH solution contains (1→3)-α-D-glucan; the chitosan is on the mycelia residuum. After isolation, the recovery yield of ergosterol is 0.52% of dry mycelium. That of (1→3)-α-D-glucan is about 8.2%; and chitosan is 5.7% with 86%deacetylation. The compositions have been characterized by IR, HPLC analyses.
