哈尔滨医科大学学报
哈爾濱醫科大學學報
합이빈의과대학학보
JOURNAL OF HARBIN MEDICAL UNIVERSITY
2009年
6期
536-538
,共3页
三氧化二砷%AGZY-83-a%凋亡%钙离子
三氧化二砷%AGZY-83-a%凋亡%鈣離子
삼양화이신%AGZY-83-a%조망%개리자
As_2O_3%AGZY-83-a%apoptosis%calcium
目的 研究三氧化二砷(As_2O_3)对人肺腺癌细胞株AGZY-83-a的抑制作用,探讨As_2O_3诱导人肺腺癌细胞株凋亡的机制.方法 采用MTT法、透射电镜及激光扫描共聚焦(LSCM)等方法 观察As_2O_3对人肺腺癌细胞株AGZY-83-a的影响.结果 As_2O_3能抑制人肺腺癌细胞株AGZY-83-a的生长,呈剂量依赖关系(P<0.01),IC_(50)=9.38μmoL/L;在光学显微镜及电镜下,可见As_2O_3,作用后的人肺腺癌细胞株AGZY-83-a呈现早期凋亡的变化特征:表面绒毛脱火,染色质块状散于核内;LSCM显示,As_2O_3,作用后人肺腺癌细胞株AGZY-83-a细胞内游离钙浓度([Ca~(2+)]_i)显著升高(P<0.01),呈剂量时间依赖关系.结论 As_2O_3能明显抑制人肺腺癌细胞株AGZY-83-a细胞的生长,并诱导人肺腺癌细胞株AGZY-83-a细胞凋亡,细胞内[Ca~(2+)]升高可能是诱导细胞凋亡的机制之一.
目的 研究三氧化二砷(As_2O_3)對人肺腺癌細胞株AGZY-83-a的抑製作用,探討As_2O_3誘導人肺腺癌細胞株凋亡的機製.方法 採用MTT法、透射電鏡及激光掃描共聚焦(LSCM)等方法 觀察As_2O_3對人肺腺癌細胞株AGZY-83-a的影響.結果 As_2O_3能抑製人肺腺癌細胞株AGZY-83-a的生長,呈劑量依賴關繫(P<0.01),IC_(50)=9.38μmoL/L;在光學顯微鏡及電鏡下,可見As_2O_3,作用後的人肺腺癌細胞株AGZY-83-a呈現早期凋亡的變化特徵:錶麵絨毛脫火,染色質塊狀散于覈內;LSCM顯示,As_2O_3,作用後人肺腺癌細胞株AGZY-83-a細胞內遊離鈣濃度([Ca~(2+)]_i)顯著升高(P<0.01),呈劑量時間依賴關繫.結論 As_2O_3能明顯抑製人肺腺癌細胞株AGZY-83-a細胞的生長,併誘導人肺腺癌細胞株AGZY-83-a細胞凋亡,細胞內[Ca~(2+)]升高可能是誘導細胞凋亡的機製之一.
목적 연구삼양화이신(As_2O_3)대인폐선암세포주AGZY-83-a적억제작용,탐토As_2O_3유도인폐선암세포주조망적궤제.방법 채용MTT법、투사전경급격광소묘공취초(LSCM)등방법 관찰As_2O_3대인폐선암세포주AGZY-83-a적영향.결과 As_2O_3능억제인폐선암세포주AGZY-83-a적생장,정제량의뢰관계(P<0.01),IC_(50)=9.38μmoL/L;재광학현미경급전경하,가견As_2O_3,작용후적인폐선암세포주AGZY-83-a정현조기조망적변화특정:표면융모탈화,염색질괴상산우핵내;LSCM현시,As_2O_3,작용후인폐선암세포주AGZY-83-a세포내유리개농도([Ca~(2+)]_i)현저승고(P<0.01),정제량시간의뢰관계.결론 As_2O_3능명현억제인폐선암세포주AGZY-83-a세포적생장,병유도인폐선암세포주AGZY-83-a세포조망,세포내[Ca~(2+)]승고가능시유도세포조망적궤제지일.
Objective To observe the pharmacological effects of arsenic trioxide(As_2O_3)on human pulmonary adenocarcinoma AGZY-83-a cells,and try to explore its mechanisms.Methods Inhibition percentage was detected by MTT assay;apoptosis was examined by transmission electron microscope and intracellular calcium concentration was investigated by laser scanning confoeal microscope(LSCM)in human pulmonary adenocarcinoma AGZY-83-a cells.Results As_2O_3 inhibited the proliferation of AGZY83-a in dose-dependent manner(P<0.01),the IC_(50) was 9.38 μmol/L;The early and media phase of apoptosis was observed:Surfacial floss break away and chromatomsome fragments scattered within neuclei;LSCM revealed that the concentration of intracellular calcium was elevated significantly after the treatment with As_2O_3 for 24 h、48 h、72 h(P<0.01)and showed relationship of concentration-time-dependent.Conclusion As_2O_3 can inhibit the proliferation of the AGZY-83-a cells and induce apoptisis of human pulmonary adenocarcinoma inoma AGZY-83-a cells,the increase of the intracellular calcium concentration may be one of its mechanisms.
