CAJ | 학술논문

针对利用葡萄糖和木糖合成2,3-丁二醇的Klebsiella pneumoniae XJ-Li菌,优化培养基组成与发酵条件,围绕五、六碳糖共代谢的特点,探讨简单可行的代谢调控方法.结果表明,60 g/L葡萄糖和40 g/L木糖为碳源,5.75 g/L NH_4H_2PO_4为氮源,pH值维持在5.5,培养温度38℃, 2,3-丁二醇浓度可达19.24 g/L.确定了pH值调控和外源添加维生素C的调控方式,通过调节发酵过程中pH值于5.5左右,使2,3-丁二醇的产量提高了16.4%;添加60 mg/L维生素C调节培养基的氧化还原状态,可使2,3-丁二醇的产量提高44.3%,批式发酵48 h, 2,3-丁二醇终浓度可达33.47 g/L.
침대이용포도당화목당합성2,3-정이순적Klebsiella pneumoniae XJ-Li균,우화배양기조성여발효조건,위요오、륙탄당공대사적특점,탐토간단가행적대사조공방법.결과표명,60 g/L포도당화40 g/L목당위탄원,5.75 g/L NH_4H_2PO_4위담원,pH치유지재5.5,배양온도38℃, 2,3-정이순농도가체19.24 g/L.학정료pH치조공화외원첨가유생소C적조공방식,통과조절발효과정중pH치우5.5좌우,사2,3-정이순적산량제고료16.4%;첨가60 mg/L유생소C조절배양기적양화환원상태,가사2,3-정이순적산량제고44.3%,비식발효48 h, 2,3-정이순종농도가체33.47 g/L.
The production conditions of 2,3-butanediol by Klebsiella pneumoniae XJ-Li from glucose and xylose were optimized, and the regulation methods for 2,3-butanediol fermentation were also determined based on the metabolic features of hexose and pentose. The results displayed that suitable culture conditions were defined as glucose of 60 g/L, xylose of 40 g/L, ammonium dihydrogen phosphate of 5.75 g/L and pH of 5.5, and the concentration of 2,3-butanediol reached 19.24 g/L under the conditions when the strain was cultured at 38℃. The yield of 2,3-butanediol was increased by 16.4% after maintaining the pH about 5.5 in the fermentation process. And 60 mg/L of vitamin C was added into medium to regulate the redox state of system, which made the yield of 2,3-butanediol improved by 44.3% of 2,3-butanediol fermentation. 33.47 g/L of 2,3-butanediol was obtained in batch fermentation for 48 h under optimum culture conditions.