基础医学与临床
基礎醫學與臨床
기출의학여림상
BASIC MEDICAL SCIENCES AND CLINICS
2010年
3期
232-236
,共5页
微小病变%阿霉素肾病%尿蛋白%蛋白质组%质谱技术
微小病變%阿黴素腎病%尿蛋白%蛋白質組%質譜技術
미소병변%아매소신병%뇨단백%단백질조%질보기술
minimal change nephropathy%adriamycin nephropathy%urine protein%proteome%mass specrometry
目的 筛选微小病变性肾病早期尿蛋白标志物.方法 以阿霉素肾病大鼠作为该病动物模型,采用LC-MS/MS蛋白质组学技术,非标记法蛋白质相对定量方法,分别分析尿全蛋白质组及ConA偶联琼脂糖珠富集的尿糖蛋白组的变化.结果 尿全蛋白质组分析得到25个差异蛋白,分别来源于血浆蛋白、免疫炎性细胞分泌蛋白及泌尿道特异分泌蛋白等,参与不同的致病过程,如血流动力学改变、足细胞损伤及免疫功能紊乱等;尿糖蛋白质组分析得到21个差异蛋白,其中12个蛋白(57%)与未进行富集实验得到的差异蛋白不同,说明两种方法具有互补性,可以从不同角度对肾脏病变进行刻画.结论 这些差异蛋白可作为微小病变性肾病早期诊断的候选标志物,对其功能的进一步验证将有助于其发病机制的解析.
目的 篩選微小病變性腎病早期尿蛋白標誌物.方法 以阿黴素腎病大鼠作為該病動物模型,採用LC-MS/MS蛋白質組學技術,非標記法蛋白質相對定量方法,分彆分析尿全蛋白質組及ConA偶聯瓊脂糖珠富集的尿糖蛋白組的變化.結果 尿全蛋白質組分析得到25箇差異蛋白,分彆來源于血漿蛋白、免疫炎性細胞分泌蛋白及泌尿道特異分泌蛋白等,參與不同的緻病過程,如血流動力學改變、足細胞損傷及免疫功能紊亂等;尿糖蛋白質組分析得到21箇差異蛋白,其中12箇蛋白(57%)與未進行富集實驗得到的差異蛋白不同,說明兩種方法具有互補性,可以從不同角度對腎髒病變進行刻畫.結論 這些差異蛋白可作為微小病變性腎病早期診斷的候選標誌物,對其功能的進一步驗證將有助于其髮病機製的解析.
목적 사선미소병변성신병조기뇨단백표지물.방법 이아매소신병대서작위해병동물모형,채용LC-MS/MS단백질조학기술,비표기법단백질상대정량방법,분별분석뇨전단백질조급ConA우련경지당주부집적뇨당단백조적변화.결과 뇨전단백질조분석득도25개차이단백,분별래원우혈장단백、면역염성세포분비단백급비뇨도특이분비단백등,삼여불동적치병과정,여혈류동역학개변、족세포손상급면역공능문란등;뇨당단백질조분석득도21개차이단백,기중12개단백(57%)여미진행부집실험득도적차이단백불동,설명량충방법구유호보성,가이종불동각도대신장병변진행각화.결론 저사차이단백가작위미소병변성신병조기진단적후선표지물,대기공능적진일보험증장유조우기발병궤제적해석.
Objective To screen early urine protein markers for minimal change nephropathy.Methods Adriamycin nephropathy was employed as minimal change nephropathy model.Urinary protein and ConA captured glycoproteins were respectively profiled.Results By profiling urine proteome,25 differential proteins were identified.These differential proteins were from leaked plasma proteins,secreted proteins from immuno-and inflammatory cells,specifically asecreted proteins from urinary tract,and so on.They took part in different pathogenic process,eg.hemodynamic changes,podocytes injury,immunological disorder and so on.By profiling ConA-enriched urinary glycoproteome,21 differential proteins were identified,among which 12(57%) were different from the above 25 differential proteins.This indicates that the knowledge of urine glycoproteome is complementary to urine proteome in understanding kidney condition.Conclusion These differential proteins can be potential indicators of minimal change nephropathy,and can help better understand the pathogenesis by further studying their functions.
