CAJ | 학술논문

分别采用SDS-酸酚法、CTAB法、异硫氰酸胍法提取白木香愈伤组织总RNA,通过比较发现,异硫氰酸胍法提取白木香愈伤组织总RNA的效果比其他两种方法更好.利用异硫氰酸胍法提取的白木香愈伤组织总RNA具有较好的完整性,OD_(260)/OD_(280)介于1.8～2.0之间.成功建立了白木香愈伤组织总RNA快速提取方法,可为其他药用植物总RNA提取提供借鉴.
분별채용SDS-산분법、CTAB법、이류청산고법제취백목향유상조직총RNA,통과비교발현,이류청산고법제취백목향유상조직총RNA적효과비기타량충방법경호.이용이류청산고법제취적백목향유상조직총RNA구유교호적완정성,OD_(260)/OD_(280)개우1.8～2.0지간.성공건립료백목향유상조직총RNA쾌속제취방법,가위기타약용식물총RNA제취제공차감.
Total RNA extraction methods of Aquilaria sinensis (Lour.) Gilg callus were discussed in the paper. SDS-acid phenol method, CTAB method, guanidine isothiocyanate method were used to extract total RNA of Aquilaria sinensis callus. We found that guanidine isothiocyanate method could be extracted the total RNA of Aquilaria sinensis callus better than the others. The total RNA of Aquilaria sinensis callus extracted by Guanidine isothiocyanate method had good integrity,the OD_(260)/OD_(280) range between 1.8～2.0. The quickly total RNA extraction method of Aquilaria sinensis callus had been sussessfully established, which could privide some advise to the total RNA extract from other medical plant.