CAJ | 학술논문

目的观察百草枯(PQ)急性中毒日本大耳白兔血液灌流后血药浓度及组织病理学改变.方法 16只日本大耳白兔随机分为PQ中毒组(8只)及血液灌流治疗组(8只),两组动物以PQ 50 mg/kg染毒,染毒后45 min左右予血液灌流治疗,两组均在不同处理后0.5、1.0、1.5、2.0、3.0、6.0、12.0、24.0、48.0、72.0 h取血检测血浆中PQ浓度；取不同处理后1、3、7d动物的肺、肝脏、肾组织,光学显微镜下观察组织病理学改变,同时观察动物的临床表现.结果治疗组动物的临床中毒表现比中毒组轻,每组均有6只动物存活至7d观察期满.与中毒组比较,治疗组1.0、1.5、2.0、3.0、6.0、12.0、24.0、48.0、72.0 h的血浆中PQ浓度均明显降低,差异有统计学意义(P＜0.05,P＜0.01).治疗组兔经HP治疗后的峰浓度、曲线下面积、半减期[分别为(5.01±0.15) μg/L、(54.03±5.31)mg·h-1·L-1、(16.29±3.26) h]均明显低于中毒组[分别为(11.97±0.75) μg/L、(141.40±10.10) mg·h-1·L-1、(31.16±9.85)h],差异有统计学意义(P＜0.05)；治疗组兔经HP治疗后的表观分布容积和清除率明显高于中毒组,差异均有统计学意义(P＜0.05).光学显微镜观察下,中毒组兔肺、肝脏、肾组织均可见充血、水肿、细胞浸润等病理学改变,治疗组兔的组织病理学改变均明显减轻.与中毒组比较,治疗组1、3、7d的肺组织、肝组织及肾小管损伤病理积分均明显降低,差异有统计学意义(P＜0.05).结论日本大耳白兔PQ急性中毒时毒物吸收快,毒性大,半减期长,早期血液灌流可有效清除血浆中毒物,减轻主要脏器的病理损害,可能有利于进一步治疗.
목적 관찰백초고(PQ)급성중독일본대이백토혈액관류후혈약농도급조직병이학개변.방법 16지일본대이백토수궤분위PQ중독조(8지)급혈액관류치료조(8지),량조동물이PQ 50 mg/kg염독,염독후45 min좌우여혈액관류치료,량조균재불동처리후0.5、1.0、1.5、2.0、3.0、6.0、12.0、24.0、48.0、72.0 h취혈검측혈장중PQ농도；취불동처리후1、3、7d동물적폐、간장、신조직,광학현미경하관찰조직병이학개변,동시관찰동물적림상표현.결과 치료조동물적림상중독표현비중독조경,매조균유6지동물존활지7d관찰기만.여중독조비교,치료조1.0、1.5、2.0、3.0、6.0、12.0、24.0、48.0、72.0 h적혈장중PQ농도균명현강저,차이유통계학의의(P＜0.05,P＜0.01).치료조토경HP치료후적봉농도、곡선하면적、반감기[분별위(5.01±0.15) μg/L、(54.03±5.31)mg·h-1·L-1、(16.29±3.26) h]균명현저우중독조[분별위(11.97±0.75) μg/L、(141.40±10.10) mg·h-1·L-1、(31.16±9.85)h],차이유통계학의의(P＜0.05)；치료조토경HP치료후적표관분포용적화청제솔명현고우중독조,차이균유통계학의의(P＜0.05).광학현미경관찰하,중독조토폐、간장、신조직균가견충혈、수종、세포침윤등병이학개변,치료조토적조직병이학개변균명현감경.여중독조비교,치료조1、3、7d적폐조직、간조직급신소관손상병리적분균명현강저,차이유통계학의의(P＜0.05).결론 일본대이백토PQ급성중독시독물흡수쾌,독성대,반감기장,조기혈액관류가유효청제혈장중독물,감경주요장기적병리손해,가능유리우진일보치료.
Objective To observe the effects of hemoperfusion on plasma concentration and histopathological chauges in paraquat (PQ) poisoning rabbits.Methods Sixteen rabbits were randomly divided into exposure group (PQ group,n=8 ) and hemopefusion plus PQ exposure group (HPQ group,n=8 ).HPQ group were given hemoperfusion in 45 min after exposure to PQ.The plasma PQ concentrations at 0.5,1.0,1.5,2.0,3.0,6.0,12.0,24.0,48.0 and 72.0 hours after exposure were measure in 2 groups.The histopathological changes of lung,liver and kidney were examined,the behavior changes and the survival number of 7 days were observed.Results The poisoning symptoms of HPQ group were generally better than those of PQ group,in each group six animals survived for 7d.The plasma PQ concentrations at 1.0,1.5,2.0,3.0,6.0,12.0,24.0,48.0,72.0 h after exposure in HPQ group were significantly lower than those in PQ group (P＜0.05 or P＜0.01 ).In HPQ group,the plasma PQ peak concentration [(5.01±0.15] μg/L],area under the curve [(54.03±5.31 ) mg·h-1·L-1] and PQ half-life time [(16.29±3.26) h] after treatment of HP were significantly lower than those [( 11.97±0.75 ) μg/L,( 141.40±10.10) mg·h-1,L-1 and (31.16±9.85 ) h] in PQ group (P＜0.05).The apparent volume of distribution and PQ clearance rate in HPQ group were significantly higher than those in PQ group (P＜0.05).Congestion,edema,cell infiltration and other pathological changes were found in lung,liver and kidney in PQ group under the light microscope,which were significantly more severe than those in HPQ group.The pathologic scores of lung tissue,liver and renal tubular damage on the 1 st,3rd,7th days after exposure in HPQ group were significantly lower than those in PQ group (P＜0.05).Conclusion When acute PQ poising,rabbits appeared the quick absorption,high toxicity and long half-life time of PQ.The early hemoperfusion can effectively remove the toxicant in plasma and reduce the pathological injury in major organs,which may be beneficial for further treatment.