CAJ | 학술논문

目的探讨白细胞介素-6(IL-6)在大鼠切割痛后脊髓中的表达及作用.方法采用纵行切割大鼠后足作为疼痛模型,取切割后不同时间点(1、6、24、72h)及假手术组(未行手术组)大鼠腰髓节段采用实时定量PCR的方法观察IL-6 mRNA的水平,并运用免疫组织化学与免疫荧光双标染色的方法,观察IL-6在腰段脊髓的表达及细胞定位；鞘内注射IL-6抗体中和内源性IL-6后,采用Von Frey尼龙纤维刺激后足以检测大鼠后足机械痛敏.结果大鼠后足切割1、6、24 h后,其同侧腰段脊髓IL-6 mRNA水平较假手术组分别上调1.43、1.74及1.46倍(t=3.315,P=0.016；t=5.121,P=0.0022;t=2.933,P=0.026),免疫组织化学结果显示切割后6 h IL-6在切割侧腰段脊髓后角表达明显增加(t=4.496,P＜0.05),并且主要表达于神经元和内皮细胞中；鞘内给予IL-6抗体可明显增加大鼠后足切割后的缩足阈值.结论 IL-6可能参与了大鼠后足切割后机械痛敏的过程,抑制IL-6的表达上调可能成为术后疼痛治疗的新靶点.
목적 탐토백세포개소-6(IL-6)재대서절할통후척수중적표체급작용.방법 채용종행절할대서후족작위동통모형,취절할후불동시간점(1、6、24、72h)급가수술조(미행수술조)대서요수절단채용실시정량PCR적방법관찰IL-6 mRNA적수평,병운용면역조직화학여면역형광쌍표염색적방법,관찰IL-6재요단척수적표체급세포정위；초내주사IL-6항체중화내원성IL-6후,채용Von Frey니룡섬유자격후족이검측대서후족궤계통민.결과 대서후족절할1、6、24 h후,기동측요단척수IL-6 mRNA수평교가수술조분별상조1.43、1.74급1.46배(t=3.315,P=0.016；t=5.121,P=0.0022;t=2.933,P=0.026),면역조직화학결과현시절할후6 h IL-6재절할측요단척수후각표체명현증가(t=4.496,P＜0.05),병차주요표체우신경원화내피세포중；초내급여IL-6항체가명현증가대서후족절할후적축족역치.결론 IL-6가능삼여료대서후족절할후궤계통민적과정,억제IL-6적표체상조가능성위술후동통치료적신파점.
Objective To investigate the expression of interleukin-6 (IL-6) and its role in incisional pain in the rat model.Methods Incisional pain rat model was established by a longitudinal incision in right plantar hind paw of isoflurane-anesthetized rats.Spinal cords were removed at various postoperative times (1,6,24,72 h).The gene expression of IL-6 in the spinal cord was determined by a Real-time PCR.Immunohistochemistry and double-labeling immunofluorescence were performed to determine the expression of IL-6 after incision.Intrathecal injection of IL-6 antibodies was performed to modulate the spinal IL-6 after surgical incision,and the mechanical hypersensitivity was assessed by paw withdrawal thresholds from mechanical stimuli of von Frey filaments.Results After hind-paw incision,the IL-6 mRNA level was upregulated in the ipsilatcral lumbar spinal cord.The immunoreactivity of IL-6 in spinal cord was increased after hind-paw incision at 6 hours after incision.The upregulated spinal IL-6 was mainly localized in the neurons and endothelial cells.Intrathecal injection of IL-6 antibodies dramatically inhibited the surgical incision that evoked pain hypersensitivity.Conclusions The present study showed that the upregulated IL-6 in the spinal cord contributed to the pain hypersensitivity after surgical incision,inhibiting the upregulated IL-6 in the spinal cord may be a possible new therapeutic target for the treatment of postoperative pain.