中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2009年
1期
16-20
,共5页
三氯乙烯%皮炎,变应性接触性%细胞色素C
三氯乙烯%皮炎,變應性接觸性%細胞色素C
삼록을희%피염,변응성접촉성%세포색소C
Trichloroethylene%Dermatitis,allergic contact%Cytochrome C
目的 研究细胞凋亡在三氯乙烯(TCE)引起BALB/c裸鼠皮肤损伤中的作用并探讨其机制.方法 将30只BALB/c裸鼠随机分为溶剂对照组、10%的TCE剂量组、20%TCE组、40%TCE组、80%TCE组、100%TCE组,应用TUNEL法检测TCE染毒的30只BALB/c裸鼠皮肤组织的细胞凋亡指数,应用免疫组化S-P法检测皮肤组织中easpase-8和cyt-c的表达.结果 电镜结果 显示高剂量组出现细胞调亡.40%、80%、100%TCE剂量组凋亡指数与对照组的差异有统计学意义(P<0.01);20%、40%、80%、100%TCE剂量组cyt c的表达水平分别为(2.60±0.54)、(3.42±0.56)、(5.81±1.30)、(6.00±0.70)明显高于对照组,差异均有统计学意义(P<0.01),各组caspase-8表达差异无统计学意义(P>0.05).结论 TCE引起的早期皮肤组织损伤与细胞凋亡有关,而细胞凋亡可能与线粒体损伤有关.
目的 研究細胞凋亡在三氯乙烯(TCE)引起BALB/c裸鼠皮膚損傷中的作用併探討其機製.方法 將30隻BALB/c裸鼠隨機分為溶劑對照組、10%的TCE劑量組、20%TCE組、40%TCE組、80%TCE組、100%TCE組,應用TUNEL法檢測TCE染毒的30隻BALB/c裸鼠皮膚組織的細胞凋亡指數,應用免疫組化S-P法檢測皮膚組織中easpase-8和cyt-c的錶達.結果 電鏡結果 顯示高劑量組齣現細胞調亡.40%、80%、100%TCE劑量組凋亡指數與對照組的差異有統計學意義(P<0.01);20%、40%、80%、100%TCE劑量組cyt c的錶達水平分彆為(2.60±0.54)、(3.42±0.56)、(5.81±1.30)、(6.00±0.70)明顯高于對照組,差異均有統計學意義(P<0.01),各組caspase-8錶達差異無統計學意義(P>0.05).結論 TCE引起的早期皮膚組織損傷與細胞凋亡有關,而細胞凋亡可能與線粒體損傷有關.
목적 연구세포조망재삼록을희(TCE)인기BALB/c라서피부손상중적작용병탐토기궤제.방법 장30지BALB/c라서수궤분위용제대조조、10%적TCE제량조、20%TCE조、40%TCE조、80%TCE조、100%TCE조,응용TUNEL법검측TCE염독적30지BALB/c라서피부조직적세포조망지수,응용면역조화S-P법검측피부조직중easpase-8화cyt-c적표체.결과 전경결과 현시고제량조출현세포조망.40%、80%、100%TCE제량조조망지수여대조조적차이유통계학의의(P<0.01);20%、40%、80%、100%TCE제량조cyt c적표체수평분별위(2.60±0.54)、(3.42±0.56)、(5.81±1.30)、(6.00±0.70)명현고우대조조,차이균유통계학의의(P<0.01),각조caspase-8표체차이무통계학의의(P>0.05).결론 TCE인기적조기피부조직손상여세포조망유관,이세포조망가능여선립체손상유관.
Objective To investigate the expression of apoptosis and caspase-8,cyt c in the skin of the BALB/c mice exposed to triehloroethylene(TCE ).Methods 30 BALB/c mice were divided in random into the solvent control group,10% TCE group,20% TCE group,40% TCE group,80% TCE group and 100% TCE group.Apoptotic cells were detected by TUNEL and EM.The expressions of caspase-8 and eyt c were detected with immunohistochemical method.Results EM showed that the apoptosis of cells was found in the high dosage groups.The immunohistochemical results showed that there were significant differences in the apoptosis rate and the activity of cyt c between the different dosage groups.There was the significant difference in the apoptosis rate between the 40%,80%,100% TCE groups the control group(P<0.01).There was the significant difference in the expression of cyt c between the 20%,40%,80%,100% TCE groups [(2.60±0.54),(3.42± 0.56),(5.81±1.30 )and (6.00±0.70),respectively] and the control group(P<0.01 ).The expressions of caspase-8 had no significant differences (P>0.05).Conclusion Apoptosis plays an important role in trichloroethylene induced irritant injury in skin and the apoptosis may be related with the mitochundrial injury.
