中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2012年
7期
446-452
,共7页
曹慧霞%朱清%楚天舒%阎磊%邵凤民
曹慧霞%硃清%楚天舒%閻磊%邵鳳民
조혜하%주청%초천서%염뢰%소봉민
硬皮病,系统性%肾疾病%HLA-DR抗原%疾病遗传易感性
硬皮病,繫統性%腎疾病%HLA-DR抗原%疾病遺傳易感性
경피병,계통성%신질병%HLA-DR항원%질병유전역감성
Scleroderma,systemic%Kidney diseases%HLA-DR antigen%Genetic predisposition to disease
目的 探讨河南省汉族人群人类白细胞抗原( HLA )-DRB1基因多态性与系统性硬化病(SSc)及其肾损害的关联性.方法 采用巢式聚合酶链反应-直接测序分型(PCR-SBT)对81例SSc患者[其中59例合并硬皮病肾损害(SRD)]及90名健康对照的HLA-DRB1基因型进行研究分析.组间差异比较采用x2检验或四格表确切概率法,多因素之间的分析采用Logistic回归分析.结果 共发现33种HLA-DRB1等位基因,SSc患者中有27种,SRD中有22种.SSc组HLA-DRB1*040501、*110101、*150201等位基因分布频率(分别为8.64％、11.11％、8.02％)高于健康对照组(分别为1.67％、4.44％、2.22％),Logistic回归分析显示HLA-DRB1* 040501(P=0.010,OR=5.839,95％CI:1.518～22.460)、*110101(P=0.019,OR =3.060,95％CI:1.204～7.772)、*150201(P=0.010,OR=4.780,95％CI:1.444～15.821)分别与SSc的发病独立相关.SRD患者组中HLA-DRB1* 040501、*150201等位基因分布频率(分别为9.32％、7.63％)较对照组(分别为1.67％、2.22％)升高,Logistic回归分析显示HLA-DRB1* 040501(P=0.008,OR=6.363,95％CI:1.614～25.087)、*150201(P=0.030,OR =4.043,95％CI:1.147～14.243)分别与SRD的发病独立相关.结论 在河南汉族人群中HLA-DRB1* 040501、*110101、*150201可能是SSc发病的易感基因；而HLA-DRB1* 040501、*150201可能是SRD发病的易感基因.
目的 探討河南省漢族人群人類白細胞抗原( HLA )-DRB1基因多態性與繫統性硬化病(SSc)及其腎損害的關聯性.方法 採用巢式聚閤酶鏈反應-直接測序分型(PCR-SBT)對81例SSc患者[其中59例閤併硬皮病腎損害(SRD)]及90名健康對照的HLA-DRB1基因型進行研究分析.組間差異比較採用x2檢驗或四格錶確切概率法,多因素之間的分析採用Logistic迴歸分析.結果 共髮現33種HLA-DRB1等位基因,SSc患者中有27種,SRD中有22種.SSc組HLA-DRB1*040501、*110101、*150201等位基因分佈頻率(分彆為8.64％、11.11％、8.02％)高于健康對照組(分彆為1.67％、4.44％、2.22％),Logistic迴歸分析顯示HLA-DRB1* 040501(P=0.010,OR=5.839,95％CI:1.518～22.460)、*110101(P=0.019,OR =3.060,95％CI:1.204～7.772)、*150201(P=0.010,OR=4.780,95％CI:1.444～15.821)分彆與SSc的髮病獨立相關.SRD患者組中HLA-DRB1* 040501、*150201等位基因分佈頻率(分彆為9.32％、7.63％)較對照組(分彆為1.67％、2.22％)升高,Logistic迴歸分析顯示HLA-DRB1* 040501(P=0.008,OR=6.363,95％CI:1.614～25.087)、*150201(P=0.030,OR =4.043,95％CI:1.147～14.243)分彆與SRD的髮病獨立相關.結論 在河南漢族人群中HLA-DRB1* 040501、*110101、*150201可能是SSc髮病的易感基因；而HLA-DRB1* 040501、*150201可能是SRD髮病的易感基因.
목적 탐토하남성한족인군인류백세포항원( HLA )-DRB1기인다태성여계통성경화병(SSc)급기신손해적관련성.방법 채용소식취합매련반응-직접측서분형(PCR-SBT)대81례SSc환자[기중59례합병경피병신손해(SRD)]급90명건강대조적HLA-DRB1기인형진행연구분석.조간차이비교채용x2검험혹사격표학절개솔법,다인소지간적분석채용Logistic회귀분석.결과 공발현33충HLA-DRB1등위기인,SSc환자중유27충,SRD중유22충.SSc조HLA-DRB1*040501、*110101、*150201등위기인분포빈솔(분별위8.64％、11.11％、8.02％)고우건강대조조(분별위1.67％、4.44％、2.22％),Logistic회귀분석현시HLA-DRB1* 040501(P=0.010,OR=5.839,95％CI:1.518～22.460)、*110101(P=0.019,OR =3.060,95％CI:1.204～7.772)、*150201(P=0.010,OR=4.780,95％CI:1.444～15.821)분별여SSc적발병독립상관.SRD환자조중HLA-DRB1* 040501、*150201등위기인분포빈솔(분별위9.32％、7.63％)교대조조(분별위1.67％、2.22％)승고,Logistic회귀분석현시HLA-DRB1* 040501(P=0.008,OR=6.363,95％CI:1.614～25.087)、*150201(P=0.030,OR =4.043,95％CI:1.147～14.243)분별여SRD적발병독립상관.결론 재하남한족인군중HLA-DRB1* 040501、*110101、*150201가능시SSc발병적역감기인；이HLA-DRB1* 040501、*150201가능시SRD발병적역감기인.
Objective To investigate the association between polymorphism of HLA-DRB1 allele and systemic sclerosis (SSc) and scleroderma-associated renal damage in Han Chinese of Henan Province.Methods Eighty-one SSc patients and 90 normal controls were recruited in this study,among them 59 patients had renal damage (SRD).The genotyping was carried out by nest PCR-SBT and gene clone.Comparisons between groups were performed with x2 test or exact probabilities.Multivariable Logistic regression was used to evaluate the association between prevalence of SSc or SRD and the possible relevant alleles.Results Thirty-three HLA-DRB1 alleles were discovered from the specimens,including 27 in SSc specimens,and 22 in SRD.Among them,the allele frequencies of HLA-DRB1 * 040501 (8.64％), * 110101 (11.11％), * 150201(8.02％) were higher in SSc patients than those of the controls (1.67％,4.44％,2.22％ respectively).After adjusted for other factors,HLA-DRBl * 040501 (P=0.010,OR =5.839,95％CI:1.518-22.460)、* 110101(P=0.019,OR=3.060,95％CI:1.204-7.772)、* 150201(P=0.010,OR=4.780,95％CI:1.444-15.821 )were identified as independent risk factors for SSc.And the allele frequencies of HLA-DRB1*040501 (9.32％),* 150201 (7.63％) were higher in SRD patients than those of the controls (1.67％,2.22％ respectively).After adjusted for other factors,HLA-DRB1 * 040501 (P=0.008,OR=6.363,95％CI:1.614-25.087) and * 150201 (P=0.030,OR =4.043,95 ％CI:1.147-14.243 ) were identified as independent risk factors for SRD.Conclusion Our data suggest that HLA-DRB1 * 040501,* 110101,* 150201 may be susceptible genes for SSc and the HLA-DRB1 * 040501,* 150201 may be susceptible genes for SRD in Han Chinese of Henan Province.
