中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
6期
502-506
,共5页
李松%车南颖%丁志鑫%张旭霞%程君%张林波%时广利%张洁%王雪玉%李传友
李鬆%車南穎%丁誌鑫%張旭霞%程君%張林波%時廣利%張潔%王雪玉%李傳友
리송%차남영%정지흠%장욱하%정군%장림파%시엄리%장길%왕설옥%리전우
结核病%Toll/白细胞介素1受体结构域衔接蛋白%易感性%单核苷酸多态性
結覈病%Toll/白細胞介素1受體結構域銜接蛋白%易感性%單覈苷痠多態性
결핵병%Toll/백세포개소1수체결구역함접단백%역감성%단핵감산다태성
Tuberculosis%Toll-interleukin 1 receptor domain containing adaptor protein%Susceptibility%Single nucleotide polymorphisms
目的 筛选中国汉族人群Toll/白细胞介素1受体结构域衔接蛋白(TIRAP)基因编码区多态性位点,并分析其与结核病易感性的相关性.方法 在小样本中测序筛选TIRAP基因编码区多态性位点,再用连接酶特异检测技术在大样本中进行单核苷酸多态性(SNP)分型,并通过统计学方法分析基因多态性与结核病易感性的相关性.结果 共筛选到4个TIRAP基因编码区多态性位点.G394A位点在结核病病人中的突变频率高于健康人,但是该位点等位基因频率在两组人群中的差异没有统计学意义(P>0.05).G164A位点跟结核病病情有关,复治病人与健康人该位点等位基因差异具有统计学意义(P<0.05),同时肺部形成空洞病人与菌阳病人突变率均比健康人要低.结论 TIRAP 基因编码区多态性可能是中国汉族人群结核病发生发展的危险因素.
目的 篩選中國漢族人群Toll/白細胞介素1受體結構域銜接蛋白(TIRAP)基因編碼區多態性位點,併分析其與結覈病易感性的相關性.方法 在小樣本中測序篩選TIRAP基因編碼區多態性位點,再用連接酶特異檢測技術在大樣本中進行單覈苷痠多態性(SNP)分型,併通過統計學方法分析基因多態性與結覈病易感性的相關性.結果 共篩選到4箇TIRAP基因編碼區多態性位點.G394A位點在結覈病病人中的突變頻率高于健康人,但是該位點等位基因頻率在兩組人群中的差異沒有統計學意義(P>0.05).G164A位點跟結覈病病情有關,複治病人與健康人該位點等位基因差異具有統計學意義(P<0.05),同時肺部形成空洞病人與菌暘病人突變率均比健康人要低.結論 TIRAP 基因編碼區多態性可能是中國漢族人群結覈病髮生髮展的危險因素.
목적 사선중국한족인군Toll/백세포개소1수체결구역함접단백(TIRAP)기인편마구다태성위점,병분석기여결핵병역감성적상관성.방법 재소양본중측서사선TIRAP기인편마구다태성위점,재용련접매특이검측기술재대양본중진행단핵감산다태성(SNP)분형,병통과통계학방법분석기인다태성여결핵병역감성적상관성.결과 공사선도4개TIRAP기인편마구다태성위점.G394A위점재결핵병병인중적돌변빈솔고우건강인,단시해위점등위기인빈솔재량조인군중적차이몰유통계학의의(P>0.05).G164A위점근결핵병병정유관,복치병인여건강인해위점등위기인차이구유통계학의의(P<0.05),동시폐부형성공동병인여균양병인돌변솔균비건강인요저.결론 TIRAP 기인편마구다태성가능시중국한족인군결핵병발생발전적위험인소.
Objective To detect specific polymorphisms in Toll-interleukin 1 receptor domain containing adaptor protein(TIRAP) coding region for Chinese Han population, and verify whether they are associated with susceptibility to tuberculosis. Methods Search TIRAP polymorphisms by sequencing in small sample; detect single nucleotide polymorphism(SNP) by ligase detection reaction technique in large sample; analyze whether polymorphisms are related to tuberculosis by statistic methods. Results Four polymorphisms were present in the TIRAP coding region. 394A had higher frequencies in the tuberculosis(TB)group than the control. But allelic and genotypic analysis showed that there were no significant difference in statistic between TB patients and controls(P>0.05). The SNP G164A mutation related with TB patient's condition. Comparing to controls, retreatment patients' allelic frequencies had significant difference in statistic(P<0.05), sputum positive patients and lung cavitation patients had lower 164A frequencies. Conclusion TIRAP coding region polymorphisms may be risk factors for TB occurrence and development in Chinese Han population.
