CAJ | 학술논문

目的观察肝母细胞瘤组织中Survivin mRNA与蛋白表达.方法采用逆转录-聚合酶链反应(RT-PCR)方法检测16例肝母细胞瘤及相应癌旁组织中Survivin mRNA的表达,免疫组织化学SP法及Western blot法检测Survivin蛋白在肝母细胞瘤中的表达.结果在肝母细胞瘤及其相应的癌旁组织中Survivin mRNA表达的阳性率分别为62.5％和11.1％,Survivin蛋白表达的阳性率分别为81.3％和22.2％,肝母细胞瘤组均远高于癌旁组,差异有统计学意义(P＜0.01)；Survivin蛋白在肝母细胞瘤组织中的表达随肿瘤分期增高而增加；Ⅲ、Ⅳ期肝母细胞瘤组织Survivin mRNA 的表达量分别为2.390±0.071和4.506±0.309,明显高于早期肝母细胞瘤组织(P＜0.05).结论 Survivin 基因的高表达可能在肝母细胞瘤的发生及发展中起重要作用.
목적 관찰간모세포류조직중Survivin mRNA여단백표체.방법 채용역전록-취합매련반응(RT-PCR)방법검측16례간모세포류급상응암방조직중Survivin mRNA적표체,면역조직화학SP법급Western blot법검측Survivin단백재간모세포류중적표체.결과 재간모세포류급기상응적암방조직중Survivin mRNA표체적양성솔분별위62.5％화11.1％,Survivin단백표체적양성솔분별위81.3％화22.2％,간모세포류조균원고우암방조,차이유통계학의의(P＜0.01)；Survivin단백재간모세포류조직중적표체수종류분기증고이증가；Ⅲ、Ⅳ기간모세포류조직Survivin mRNA 적표체량분별위2.390±0.071화4.506±0.309,명현고우조기간모세포류조직(P＜0.05).결론 Survivin 기인적고표체가능재간모세포류적발생급발전중기중요작용.
Objective To observe the expression of Survivin mRNA and protein in hepatoblastoma.Methods The expression of Survivin mRNA in hepatoblastoma was detected by using real-time polymerase chain reaction (PCR),and that of Survivin protein by immunohistochemistry and Western blotting in hepatoblastoma tissues.Results The positive expression rate of Survivin mRNA in hepatoblastoma tissues and paraneoplastic tissues was 62.5％ and 11.1％ respectively,and that of Survivin protein was 81.3％ and 22.2％ respectively ( P ＜0.01 ).Western blotting analysis showed a significant correlation between Survivin protein expression and clinical stages (P ＜0.05 ).The expression of Survivin mRNA in Ⅲ and Ⅳ clinical stage was 2.390 ± 0.071 and 4.506 ± 0.309 respectively,obviously higher than that in earlier stages.Conclusion High expression of Survivin may play an important role in the genesis and development of hepatoblastoma.