中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2008年
7期
657-660
,共4页
潘杰香%李飞%储卫华%冯华
潘傑香%李飛%儲衛華%馮華
반걸향%리비%저위화%풍화
高压氧治疗%大脑中动脉闭塞%巢蛋白
高壓氧治療%大腦中動脈閉塞%巢蛋白
고압양치료%대뇌중동맥폐새%소단백
Hyperbaric oxygen treatment%Middle cerebral artery occlusion%Nestin
目的 探讨高压氧(HBO)治疗对兔大脑中动脉闭塞(MCAO)模型梗死灶周围巢蛋白(nestin)表达的影响.方法 制备兔右侧MCAO模型.将动物分为3组,即MCAO+HBO治疗组(30只):MCAO后立即行HBO治疗,每天1次,连续20 d;MCAO对照组(30只):MCAO后不行HBO治疗;假手术组(10只):仅开颅不闭塞血管.分别于MCAO后10d、20 d观察其行为学、梗死体积变化.并用免疫组化法检测距梗死灶中心3 mm内皮层脑组织内nestin的表达.结果 MCAO后.MCAO+HBO治疗组行为学评分高于MCAO对照组,差异有统计学意义(P<0.05);MCAO+HBO治疗组脑梗死体积小于MCAO对照组,差异有统计学意义(P<0.05);MACO后10 d、20 dMCAO+HBO治疗组nestin免疫阳性细胞数05.88±1.2、20.03±1.6)高于MCAO对照组(6.63±1.6、6.82±0.8),差异有统计学意义(P<0.05).结论 早期、连续20 d HBO治疗对兔MCAO后的神经行为学、梗死体积有明显的治疗效果,并促进兔MCAO后梗死灶周围脑组织内nestin的表达.
目的 探討高壓氧(HBO)治療對兔大腦中動脈閉塞(MCAO)模型梗死竈週圍巢蛋白(nestin)錶達的影響.方法 製備兔右側MCAO模型.將動物分為3組,即MCAO+HBO治療組(30隻):MCAO後立即行HBO治療,每天1次,連續20 d;MCAO對照組(30隻):MCAO後不行HBO治療;假手術組(10隻):僅開顱不閉塞血管.分彆于MCAO後10d、20 d觀察其行為學、梗死體積變化.併用免疫組化法檢測距梗死竈中心3 mm內皮層腦組織內nestin的錶達.結果 MCAO後.MCAO+HBO治療組行為學評分高于MCAO對照組,差異有統計學意義(P<0.05);MCAO+HBO治療組腦梗死體積小于MCAO對照組,差異有統計學意義(P<0.05);MACO後10 d、20 dMCAO+HBO治療組nestin免疫暘性細胞數05.88±1.2、20.03±1.6)高于MCAO對照組(6.63±1.6、6.82±0.8),差異有統計學意義(P<0.05).結論 早期、連續20 d HBO治療對兔MCAO後的神經行為學、梗死體積有明顯的治療效果,併促進兔MCAO後梗死竈週圍腦組織內nestin的錶達.
목적 탐토고압양(HBO)치료대토대뇌중동맥폐새(MCAO)모형경사조주위소단백(nestin)표체적영향.방법 제비토우측MCAO모형.장동물분위3조,즉MCAO+HBO치료조(30지):MCAO후립즉행HBO치료,매천1차,련속20 d;MCAO대조조(30지):MCAO후불행HBO치료;가수술조(10지):부개로불폐새혈관.분별우MCAO후10d、20 d관찰기행위학、경사체적변화.병용면역조화법검측거경사조중심3 mm내피층뇌조직내nestin적표체.결과 MCAO후.MCAO+HBO치료조행위학평분고우MCAO대조조,차이유통계학의의(P<0.05);MCAO+HBO치료조뇌경사체적소우MCAO대조조,차이유통계학의의(P<0.05);MACO후10 d、20 dMCAO+HBO치료조nestin면역양성세포수05.88±1.2、20.03±1.6)고우MCAO대조조(6.63±1.6、6.82±0.8),차이유통계학의의(P<0.05).결론 조기、련속20 d HBO치료대토MCAO후적신경행위학、경사체적유명현적치료효과,병촉진토MCAO후경사조주위뇌조직내nestin적표체.
Objective To evaluate the effect of hyperbaric oxygen (HBO) treatment on the expression of nestin in a model of right middle cerebral artery occlusion (MCAO) in rabbits. Methods Seventy healthy rabbits were dividod into 3 groups: the rabbits in MCAO+HBO group(n=30) were treated with HBO immediately after MCAO, once a day, consecutively for 20 d; the rabbits in MCAO group (n=30) were not trcated with any therapy after MCAO; the control group (n=10) received the same operation as that of MCAO group except MCAO. Rabbit behaviors and volumes of infarction were observed, and the expression of nestin was measured by immunocytochernistry 10 and 20 d after MCAO. Results Behaviors' scores and volumes of infarction were better in MCAO+HBO group than the MCAO group (P<0.05). 10 d after MCAO, the number of nestin-immunoreactive cells of MCAO+HBO group and MCAO group was respectively 15.88±1.2 and 6.63±1.6; 20 d after MCAO, the number in MCAO+HBO group and MCAO group was respectively 20.03±1.6 and 6.82±0.8. The number of nestin-immunoreactive cells in MCAO+HBO group was significantly higher compared with that of MCAO group (P<0.05). Conclusion Early applications of HBO and repeated administration of HBO for 20 d can evidently improve the behaviors and infarct size and elevate the expression of nestin in rabbit models of MCAO.
