CAJ | 학술논문

目的观察小鼠胰腺导管上皮细胞向胰岛样细胞转分化及其在治疗糖尿病小鼠中的作用.方法分离培养昆明小鼠胰腺导管上皮细胞,经体外扩增及诱导培养后,进行体外和体内功能评价.结果小鼠胰腺导管上皮细胞经体外扩增和诱导分化后,逆转录-聚合酶链反应(RT-PCR)显示培养1周和2周后胰岛素mRNA的IA值为(1.892±0.119比3.135±0.092,P＜0.05),胰高血糖素为(1.564±0.087比2.271±0.042,P＜0.05),表达明显上调;胰岛样细胞对高糖刺激(15.0 mmol/L)的胰岛素释放较低糖(5.6 mmol/L)时增加了1.7倍[(52.3±10.5)mmol/L比(30.2±9.7)mmol/L,P＜0.05];DTZ染色阳性;将其移植入糖尿病小鼠体内后,移植组小鼠较对照组血糖下降,差异有统计学意义(P＜0.05).结论昆明小鼠胰腺导管上皮细胞在体外培养条件可转分化胰岛素分泌细胞,该细胞团可在体内环境下发挥生物学功能.
목적 관찰소서이선도관상피세포향이도양세포전분화급기재치료당뇨병소서중적작용.방법 분리배양곤명소서이선도관상피세포,경체외확증급유도배양후,진행체외화체내공능평개.결과 소서이선도관상피세포경체외확증화유도분화후,역전록-취합매련반응(RT-PCR)현시배양1주화2주후이도소mRNA적IA치위(1.892±0.119비3.135±0.092,P＜0.05),이고혈당소위(1.564±0.087비2.271±0.042,P＜0.05),표체명현상조;이도양세포대고당자격(15.0 mmol/L)적이도소석방교저당(5.6 mmol/L)시증가료1.7배[(52.3±10.5)mmol/L비(30.2±9.7)mmol/L,P＜0.05];DTZ염색양성;장기이식입당뇨병소서체내후,이식조소서교대조조혈당하강,차이유통계학의의(P＜0.05).결론 곤명소서이선도관상피세포재체외배양조건가전분화이도소분비세포,해세포단가재체내배경하발휘생물학공능.
Objective To investigate the differentiation of stem cells deived from mouse pancreatic ductal epithelial cells toward insulin secreting cells and the potential application for diabetes therapy.Methods Pancreatic ductal epithelial cells were separated and differentiated into islet-like cells. Study was performed to determine whether these islet-like clusters could secrete insulin in response to glucose both in vivo and in vitro. Results Pancreatic ductal epithelial cells were separated and cultured in vitro.The expression of insulin mRNA in the stem cells was significantly up-regulated ( 1. 892 ±0. 119 vs 3. 135± 0. 092,P ＜ 0. 05 ), and also in glucagon ( 1. 564 ± 0. 087 vs 2. 271 ± 0. 042, P ＜ 0. 05 ). Immunofluoresence staining indicated that there were a lot of insulin positive cells. Insulin released from cells in response to glucose stimulation in vitro was increased [ ( 52. 3 ± 10. 5 ) mmol/L vs ( 30. 2± 9. 7 ) mmol/L, P ＜0. 05 ]. Hyperglycemia in diabetic animals was alleviated after cell transplantation. Conclusion Islet-like cell clusters generated in vitro from Kunming mice pancreatic ductal epithelial cells could secrete insulin and have some effects on reversing the diabetes in diabetic mice.