中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2011年
7期
697-700
,共4页
陈宏颉%王守森%郑兆聪%袁邦青%高进喜%刘峥%王如密%季天海
陳宏頡%王守森%鄭兆聰%袁邦青%高進喜%劉崢%王如密%季天海
진굉힐%왕수삼%정조총%원방청%고진희%류쟁%왕여밀%계천해
TRAIL%U251%细胞凋亡%抗肿瘤联合化疗方案
TRAIL%U251%細胞凋亡%抗腫瘤聯閤化療方案
TRAIL%U251%세포조망%항종류연합화료방안
TRAIL%U251%Apoptosis%Antineoplastic combined chemotherapy protocols
目的 探讨TRAIL腺病毒增强胶质瘤对顺铂的化疗敏感性.方法 制备TRAIL腺病毒,转染胶质瘤U251,流式细胞仪和MTT法检测顺铂作用于U251细胞后各组细胞的凋亡率和增殖率.结果 本研究成功将腺病毒转染进胶质瘤U251细胞,其转染率为82.1%.TRAIL腺病毒转染胶质瘤U251 后,细胞凋亡率增加为(34.2±5.8),%细胞生长缓慢,基本上处于抑制状态.结论 TRAIL腺病毒能显著增强U251细胞对顺铂的敏感性,为胶质瘤的基因治疗提供了理论依据.
目的 探討TRAIL腺病毒增彊膠質瘤對順鉑的化療敏感性.方法 製備TRAIL腺病毒,轉染膠質瘤U251,流式細胞儀和MTT法檢測順鉑作用于U251細胞後各組細胞的凋亡率和增殖率.結果 本研究成功將腺病毒轉染進膠質瘤U251細胞,其轉染率為82.1%.TRAIL腺病毒轉染膠質瘤U251 後,細胞凋亡率增加為(34.2±5.8),%細胞生長緩慢,基本上處于抑製狀態.結論 TRAIL腺病毒能顯著增彊U251細胞對順鉑的敏感性,為膠質瘤的基因治療提供瞭理論依據.
목적 탐토TRAIL선병독증강효질류대순박적화료민감성.방법 제비TRAIL선병독,전염효질류U251,류식세포의화MTT법검측순박작용우U251세포후각조세포적조망솔화증식솔.결과 본연구성공장선병독전염진효질류U251세포,기전염솔위82.1%.TRAIL선병독전염효질류U251 후,세포조망솔증가위(34.2±5.8),%세포생장완만,기본상처우억제상태.결론 TRAIL선병독능현저증강U251세포대순박적민감성,위효질류적기인치료제공료이론의거.
Objective To investigate the effect of adenovirus TRAIL on the sensitivity of glioma cells to DDP chemotherapy. Method The adenovirus TRAIL was prepared and transduced into U251 glioma cells. After DDP treatment, the apoptosis and viability of glioma cells were analyzed by FCM and MTT assays. Results The adenovirus could transduce into U251 cell with the efficiency of 82. 1 %. After adenovirus TRAIL transduction, the apoptosis rate of glioma cells was increased to ( 34. 2 ± 5. 8) % , and the cell proliferation was inhibited after DDP treatment Conclusions Adenovirus TRAIL could increase the apoptosis and sensitivity of glioma cells to chemotherapy,which may povide an ideal strategy for glioma cell gene theray.
