中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
3期
404-407
,共4页
李桢%梁炜%张红亚%李国华%王来奎%王云峰
李楨%樑煒%張紅亞%李國華%王來奎%王雲峰
리정%량위%장홍아%리국화%왕래규%왕운봉
胃旁路术%吻合术/Roux-en-Y%糖尿病%肾功能
胃徬路術%吻閤術/Roux-en-Y%糖尿病%腎功能
위방로술%문합술/Roux-en-Y%당뇨병%신공능
Gastric bypass%Anastomasis/Roux-en-Y%Diabetes mellitus%Kidney function
目的 观察Roux-en-Y胃旁路术对糖尿病Goto-Kakizaki (GK)大鼠肾脏的保护作用.方法 18只GK大鼠随机等分为Roux-en-Y胃旁路手术组(RYGP组)、假RYGP组和对照组；观察手术前、手术后12周各组大鼠血清血栓素(TXB2)、6-酮-前列环素(6-kET-1o-PGF1a)、内皮素-1( ET-1)、尿素氮(sCr)、肌酐(BUN)和24h尿蛋白(24 h uPro)含量；手术后12周苏木素-伊红(HE)染色观察大鼠肾脏病理变化.结果 手术前3组大鼠各检测指标差异无统计学意义(P＞0.05).与手术前比较,手术后12周,假RYGP组大鼠TXB2(66.31±6.13) ng/L比(101.42 ±9.70) ng/L、ET-1 (58.31±5.32) ng/L比(79.01±6.89) ng/L、sCr( 48.78±3.66) μmol/L比(70.33±6.21)μmol/L、BUN(5.41±0.68) mmol/L比(8.35±0.92) mmol/L和24h uPro (0.44±0.10) g/24 h比(0.86 ±0.17) g/24 h均显著升高,6-kET-1o-PGF1a(85.72±6.87) ng/L比(60.41±8.23) ng/L显著降低,差异均有统计学意义(P＜0.05)；对照组与假RYGP组一样获得相似的结果；RYGP组大鼠各检测指标变化差异无统计学意义(P＞0.05).手术后12周,RYGP组与假RYGP组比较TXB2(72.31±7.56) ng/L比(101.42±9.70)ng/L、ET-1 (62.11±6.26) ng/L比(79.01 ±6.89)ng/L、sCr (54.36±4.12)μmol/L比(70.33±6.21)μmol/L、BUN( 5.71±0.86) mmol/L比(8.35±0.92) mmoL/L、24 h uPro(0.52 ±0.10) g/24 h比(0.86±0.17)g/24 h和6-kET-1o-PGF1a( 83.22±5.62) ng/L比(60.41±8.23) ng/L差异均有统计学意义(P＜0.05)；RYGP组与对照组比较,各检测指标差异均有统计学意义(P＜0.05)；假RYGP组、对照组血清TXB2与6-kET-1o-PGF1a呈显著负相关,与ET-1呈显著正相关(P＜0.05).手术后12周,假RYGP组和对照组大鼠肾脏有明显病理性损害；RYGP组大鼠肾脏病理变化不明显.结论 Roux-en-Y胃旁路术对GK大鼠肾脏具有保护作用.
目的 觀察Roux-en-Y胃徬路術對糖尿病Goto-Kakizaki (GK)大鼠腎髒的保護作用.方法 18隻GK大鼠隨機等分為Roux-en-Y胃徬路手術組(RYGP組)、假RYGP組和對照組；觀察手術前、手術後12週各組大鼠血清血栓素(TXB2)、6-酮-前列環素(6-kET-1o-PGF1a)、內皮素-1( ET-1)、尿素氮(sCr)、肌酐(BUN)和24h尿蛋白(24 h uPro)含量；手術後12週囌木素-伊紅(HE)染色觀察大鼠腎髒病理變化.結果 手術前3組大鼠各檢測指標差異無統計學意義(P＞0.05).與手術前比較,手術後12週,假RYGP組大鼠TXB2(66.31±6.13) ng/L比(101.42 ±9.70) ng/L、ET-1 (58.31±5.32) ng/L比(79.01±6.89) ng/L、sCr( 48.78±3.66) μmol/L比(70.33±6.21)μmol/L、BUN(5.41±0.68) mmol/L比(8.35±0.92) mmol/L和24h uPro (0.44±0.10) g/24 h比(0.86 ±0.17) g/24 h均顯著升高,6-kET-1o-PGF1a(85.72±6.87) ng/L比(60.41±8.23) ng/L顯著降低,差異均有統計學意義(P＜0.05)；對照組與假RYGP組一樣穫得相似的結果；RYGP組大鼠各檢測指標變化差異無統計學意義(P＞0.05).手術後12週,RYGP組與假RYGP組比較TXB2(72.31±7.56) ng/L比(101.42±9.70)ng/L、ET-1 (62.11±6.26) ng/L比(79.01 ±6.89)ng/L、sCr (54.36±4.12)μmol/L比(70.33±6.21)μmol/L、BUN( 5.71±0.86) mmol/L比(8.35±0.92) mmoL/L、24 h uPro(0.52 ±0.10) g/24 h比(0.86±0.17)g/24 h和6-kET-1o-PGF1a( 83.22±5.62) ng/L比(60.41±8.23) ng/L差異均有統計學意義(P＜0.05)；RYGP組與對照組比較,各檢測指標差異均有統計學意義(P＜0.05)；假RYGP組、對照組血清TXB2與6-kET-1o-PGF1a呈顯著負相關,與ET-1呈顯著正相關(P＜0.05).手術後12週,假RYGP組和對照組大鼠腎髒有明顯病理性損害；RYGP組大鼠腎髒病理變化不明顯.結論 Roux-en-Y胃徬路術對GK大鼠腎髒具有保護作用.
목적 관찰Roux-en-Y위방로술대당뇨병Goto-Kakizaki (GK)대서신장적보호작용.방법 18지GK대서수궤등분위Roux-en-Y위방로수술조(RYGP조)、가RYGP조화대조조；관찰수술전、수술후12주각조대서혈청혈전소(TXB2)、6-동-전렬배소(6-kET-1o-PGF1a)、내피소-1( ET-1)、뇨소담(sCr)、기항(BUN)화24h뇨단백(24 h uPro)함량；수술후12주소목소-이홍(HE)염색관찰대서신장병리변화.결과 수술전3조대서각검측지표차이무통계학의의(P＞0.05).여수술전비교,수술후12주,가RYGP조대서TXB2(66.31±6.13) ng/L비(101.42 ±9.70) ng/L、ET-1 (58.31±5.32) ng/L비(79.01±6.89) ng/L、sCr( 48.78±3.66) μmol/L비(70.33±6.21)μmol/L、BUN(5.41±0.68) mmol/L비(8.35±0.92) mmol/L화24h uPro (0.44±0.10) g/24 h비(0.86 ±0.17) g/24 h균현저승고,6-kET-1o-PGF1a(85.72±6.87) ng/L비(60.41±8.23) ng/L현저강저,차이균유통계학의의(P＜0.05)；대조조여가RYGP조일양획득상사적결과；RYGP조대서각검측지표변화차이무통계학의의(P＞0.05).수술후12주,RYGP조여가RYGP조비교TXB2(72.31±7.56) ng/L비(101.42±9.70)ng/L、ET-1 (62.11±6.26) ng/L비(79.01 ±6.89)ng/L、sCr (54.36±4.12)μmol/L비(70.33±6.21)μmol/L、BUN( 5.71±0.86) mmol/L비(8.35±0.92) mmoL/L、24 h uPro(0.52 ±0.10) g/24 h비(0.86±0.17)g/24 h화6-kET-1o-PGF1a( 83.22±5.62) ng/L비(60.41±8.23) ng/L차이균유통계학의의(P＜0.05)；RYGP조여대조조비교,각검측지표차이균유통계학의의(P＜0.05)；가RYGP조、대조조혈청TXB2여6-kET-1o-PGF1a정현저부상관,여ET-1정현저정상관(P＜0.05).수술후12주,가RYGP조화대조조대서신장유명현병이성손해；RYGP조대서신장병리변화불명현.결론 Roux-en-Y위방로술대GK대서신장구유보호작용.
Objective To observe the protective effects of Roux-en-Y gastric bypass on the kidney in Goto-Kakizaki (GK) diabetic rats.Methods Eighteen male GK rats were randomly divided into Rouxen-Y gastric bypass group ( RYGP),sham RYGP group (sham-RYGP) and pair-fed control group.Before and 12 weeks after operation,the values of serum thromboxane B2 (TXB2),6-kET-1o-taglandine Ia (6-kET-1o-PGF1a),endothelin-1 (ET-1),serum creatinine (sCr),blood urea nitrogen (BUN),and 24-h urine protein (24-h uPro) were measured,and the pathological changes of the kidney were analyzed 12 weeks after operation.Results No significant differences were observed in pre-operative values ( P ＞0.05) among three groups.In sham-RYGP group,levels measured before and 12 weeks after operation were as follows:TXB2 (66.31 ±6.13) ng/L vs.(101.42 ±9.70) ng/L,ET-1 (58.31 ±5.32) ng/Lvs.(79.01 ±6.89) ng/L,sCr (48.78 ±3.66) μmol/L vs.(70.33 ±6.21) μmol/L,BUN (5.41 ±0.68)mmol/L vs.(8.35 ±0.92) mmol/L and 24-h uPro (0.44 ±0.10) g/24 h vs.(0.86 ±0.17) g/24 h( all P ＜ 0.05 ) ; 6-kET-1o-PGF1a ( 85.72 ± 6.87) ng/L vs.60.41 ± 8.23 ) ng/L ( P ＜ 0.05 ).There was significant difference between sham-RYGP group and control group ( all P ＜ 0.05 ).There was no significant difference before and 12 weeks after operation in RYGP group (P＞0.05).Between RYGP group and sham-RYGP,there were significant differences in TXB2 (72.31 ±7.56) ng/L vs.( 101.42 ±9.70) ng/L,ET-1 (62.11±6.26) ng/L vs.(79.01 ±6.89) ng/L,sCr (54.36±4.12) μmol/L vs.(70.33±6.21) μmol/L,BUN (5.71 ±0.86) mmol/L vs.(8.35 ±0.92) mmol/L,24-h uPro (0.52 ±0.10)g/24 h vs.(0.86 ±0.17) g/24 h and 6-kET-1o-PGF1a (83.22 ±5.62) ng/L vs.(60.41 ±8.23) ng/Lat 12th week after operation ( all P ＜ 0.05 ),as well as between RYGP group and control group.On the post-operative week 12,serum TXB2 level was positively related with ET-1 level,but negatively with 6-kET-1o-PGF1a in sham-RYGP and control group (P ＜ 0.05 ).On the post-operative week 12,pathologic lesions were observed on the kidney of GK rats in sham-RYGP and control groups,but there was no obvious pathologi lesions of the kidney in RYGP group.Conclusion Roux-en-Y gastric bypass can protect the kidney in diabetic GK rats.
