CAJ | 학술논문

目的观察褪黑素(Mel)对大鼠肝组织caspase-3表达的影响及对肝缺血再灌注损伤的保护作用.方法将72只Wistar大鼠,随机分为褪黑素处理3、6、12、24 h组(Mel组),同样乙醇溶媒对照组(Alc组)和生理盐水对照组(NS组)也分别按3、6、12、24 h各自分为4组,总共为12组.建立肝缺血再灌注损伤模型,于不同时点测定血清肝组织生化酶:ALT、AKP、对肝组织进行Caspase-3免疫组织化学染色.结果 ALT在再灌注后各时点Mel组均显著低于Alc及NS对照组(P＜0.05),AKP在再灌注后6、12、24 h,Mel组显著低于Alc及NS对照组(P＜0.05),Mel组再灌注后各时点的Caspase-3染色阳性细胞率均显著低于对照组(Alc组和Ns组,P＜0.05),以上各项指标在各时点Alc组与NS组比较差异无统计学意义(P＞0.05).结论 Mel能够减轻大鼠肝缺血再灌注损伤时的肝功能损害,抑制肝细胞Caspase-3的表达,减少肝细胞凋亡,对大鼠肝脏缺血再灌注损伤具有保护作用.
목적 관찰퇴흑소(Mel)대대서간조직caspase-3표체적영향급대간결혈재관주손상적보호작용.방법 장72지Wistar대서,수궤분위퇴흑소처리3、6、12、24 h조(Mel조),동양을순용매대조조(Alc조)화생리염수대조조(NS조)야분별안3、6、12、24 h각자분위4조,총공위12조.건립간결혈재관주손상모형,우불동시점측정혈청간조직생화매:ALT、AKP、대간조직진행Caspase-3면역조직화학염색.결과 ALT재재관주후각시점Mel조균현저저우Alc급NS대조조(P＜0.05),AKP재재관주후6、12、24 h,Mel조현저저우Alc급NS대조조(P＜0.05),Mel조재관주후각시점적Caspase-3염색양성세포솔균현저저우대조조(Alc조화Ns조,P＜0.05),이상각항지표재각시점Alc조여NS조비교차이무통계학의의(P＞0.05).결론 Mel능구감경대서간결혈재관주손상시적간공능손해,억제간세포Caspase-3적표체,감소간세포조망,대대서간장결혈재관주손상구유보호작용.
Objective To investigate the protective effects of melatonin (Mel) on hepatic ischemia-reperfusion injury in rats.Methods Seventy-two healthy male Wistar rats (6-7 weeks old ) were randomly divided into 12 groups:Mel exposured group (Mel),alcohol solvent control group (Alc) and saline control group ( NS),and each of them into 4 subgroups of 3,6,12 and 24 h after treatment.The partial hepatic ischemia-reperfusion injury models were established,and the enzyme levels in serum were determined:including alanine aminotransferase (ALT) and alkaline phosphatase (AKP).The expression of caspase-3 was detected in liver tissues by immunohistochemical stain,and the apoptosis of the hepatic cells by electron microscopy.Results The levels of ALT in Mel group were significantly lower than in Alc and NS groups ( P＜0.05).The levels of AKP in Mel group determined at 6th,12th and 24th h were significantly lower than in Alc and NS groups ( P＜0.05 ).The expression of caspase-3 in Mel group was markedly lower than in Alc and NS groups ( P＜0.05 ).There were no significant differences in the above results between Alc and NS groups.More apoptotic ceils were observed in Acl and NS group than in Mel group.Conclusion Mel can reduce the hepatic injury due to the ischemia-reperfusion in rats,inhibit the expression of caspase-3,and alleviate the apoptosis of the hepatic cells.Mel can protect the ischema-reperfusion injury of hepatic cells.