CAJ | 학술논문

目的分析Bax基因G(-248)A多态的生物学功能,探讨其与中国人群肺癌发病风险的关系.方法 Bax基因G(-248)A的生物学功能通过报告基因实验验证.采用病例-对照研究设计,以聚合酶链扩增产物限制性片段长度多态性(PCR-RFLP)方法,分析989例肺癌患者和990名正常对照者的Bax基因G(-248)A多态基因分型,分析该多态与肺癌发病风险的关系.结果在A549、GLC82、EC9706和KYSE150细胞中,p-Bax-A的表达水平分别为2.082±0.131、2.265 4±0.168、2.029±0.068和0.617±0.033,p-Bax-G的表达水平分别为1.245±0.076、1.294±0.068、1.366±0.043和0.390±0.023,Bax基因-248A等位基因的转录活性明显高于G等位基因(均P<0.01),且显著降低了肺癌的发病风险(OR=0.68,95% CI为0.48～0.90).在轻度吸烟者中,G(-248)A多态与吸烟存在协同作用.结论 Bax基因G(-248)A多态是影响Bax表达的功能性多态,是影响肺癌易感性的遗传因素,与肺癌发病风险相关.
목적 분석Bax기인G(-248)A다태적생물학공능,탐토기여중국인군폐암발병풍험적관계.방법 Bax기인G(-248)A적생물학공능통과보고기인실험험증.채용병례-대조연구설계,이취합매련확증산물한제성편단장도다태성(PCR-RFLP)방법,분석989례폐암환자화990명정상대조자적Bax기인G(-248)A다태기인분형,분석해다태여폐암발병풍험적관계.결과 재A549、GLC82、EC9706화KYSE150세포중,p-Bax-A적표체수평분별위2.082±0.131、2.265 4±0.168、2.029±0.068화0.617±0.033,p-Bax-G적표체수평분별위1.245±0.076、1.294±0.068、1.366±0.043화0.390±0.023,Bax기인-248A등위기인적전록활성명현고우G등위기인(균P<0.01),차현저강저료폐암적발병풍험(OR=0.68,95% CI위0.48～0.90).재경도흡연자중,G(-248)A다태여흡연존재협동작용.결론 Bax기인G(-248)A다태시영향Bax표체적공능성다태,시영향폐암역감성적유전인소,여폐암발병풍험상관.
Objective The Bcl-2 associated X protein (Bax), belonging to the Bcl-2 family, plays a pivotal role in mitochondria-dependent apoptosis. The aims of this study are to revalidate the functional significance of Bax G(-248) A polymorphism, and investigate its association with lung cancer risk in Chinese population. Methods The biological function of Bax G(-248) A was tested by luciferase assays, and its effects on lung cancer risk was determined by case-control analysis of 989 patients with lung cancer and 990 controls. Results Bax-248A allele exhibited significantly higher transcriptional activity compared with C allele. The Bax-248A allele carriers yielded a significantly decreased risk of lung cancer, compared with -248G allele carriers (OR=0.68, 95% CI=0.48～0.90, P=0.01). Furthermore, a significant gene-smoking interaction between Bax G (-248) A polymorphism and smoking existed among the light smokers.Conclusion Bax G (-248) A polymorphism is associated with lung cancer susceptibility in Chinese population.