CAJ | 학술논문

目的观察主要组织相容性复合体Ⅰ类相关基因A(major histocompatibility complex class Ⅰ chain-related gene A,MICA)全长多态性与结肠癌的发病是否关联,以及血清可溶性主要组织相容性复合体Ⅰ类相关抗原A(major histocomptctibility complex class Ⅰ chain-related antigen A,MICA)浓度与结肠癌发病及病程的相关性.方法应用聚合酶链反应-序列特异性引物及DNA测序分型方法,分析江苏扬州地区117例结肠癌患者和113名健康个体的MICA基因全长多态性及其编码分子第129位氨基酸残基的变化.血清可溶性MICA浓度通过酶联免疫吸附试验方法测定.结果结肠癌患者和正常群体胞外区和跨膜区MICA等位基因的分布差异均无统计学意义,MICA跨膜区等位基因在不同病期结肠癌患者的分布差异亦无统计学意义.而结肠癌患者群体内MICA第129位氨基酸残基为蛋氨酸的频率显著降低;可溶性MICA浓度在Duke's C和D期患者血清内显著升高.结论 MICA等位基因多态性与结肠癌的发病及进展没有关联,但血清可溶性MICA浓度的检测可作为判断结肠癌预后的指标之一.
목적 관찰주요조직상용성복합체Ⅰ류상관기인A(major histocompatibility complex class Ⅰ chain-related gene A,MICA)전장다태성여결장암적발병시부관련,이급혈청가용성주요조직상용성복합체Ⅰ류상관항원A(major histocomptctibility complex class Ⅰ chain-related antigen A,MICA)농도여결장암발병급병정적상관성.방법 응용취합매련반응-서렬특이성인물급DNA측서분형방법,분석강소양주지구117례결장암환자화113명건강개체적MICA기인전장다태성급기편마분자제129위안기산잔기적변화.혈청가용성MICA농도통과매련면역흡부시험방법측정.결과 결장암환자화정상군체포외구화과막구MICA등위기인적분포차이균무통계학의의,MICA과막구등위기인재불동병기결장암환자적분포차이역무통계학의의.이결장암환자군체내MICA제129위안기산잔기위단안산적빈솔현저강저;가용성MICA농도재Duke's C화D기환자혈청내현저승고.결론 MICA등위기인다태성여결장암적발병급진전몰유관련,단혈청가용성MICA농도적검측가작위판단결장암예후적지표지일.
Objective To investigate whether the major histocompatibility complex class I chain-related gene A gene ( MICA) polymorphism and serum soluble MICA level were associated with the occurrence and development of colorectal cancer. Methods DNA samples from 117 colorectal cancer patients and 113 healthy individuals from Yangzhou in Jiangsu province were genotyped by using the polymerase chain reaction (PCR) and sequence-specific primer (SSP) method and PCR based sequencing. In addition, polymorphism at position 129 was also analyzed by PCR-SSP. Serum levels of soluble MICA were measured by a sandwich ELISA method. Results Neither the extracellular nor the transmembrane region polymorphisms of MICA gene were associated with the occurrence and the different stages of colorectal cancer. In contrast, the frequency of the methionine residue at position 129 was significantly decreased in the patient group. Soluble MICA levels in sera were increased in the late stages of colorectal cancer. Conclusion Although there was no genetic susceptibility attributed to MICA gene polymorphism with regard to development of colorectal cancer, serum levels of soluble MICA may be a diagnostic marker of advanced stages.