CAJ | 학술논문

以洋葱(Allium cepa L.) 细胞为研究材料，应用DNA细胞化学特异染色方法(NAMA_Ur)及常规电子显微镜技术，观察了洋葱细胞核仁FC(纤维中心)内DNA的超微结构，发现FC内DNA存在着一个介于集缩到解集缩之间的变化过程，并揭示了DNA在核仁内的连续排布过程，即核仁外DNA经过核仁通道进入到FC后，继续沿FC的边缘或DFC(致密纤维成分)与FC的交界处环绕FC而排布，再经FC之间的核仁通道，延伸到另外的FC区域。
이양총(Allium cepa L.) 세포위연구재료，응용DNA세포화학특이염색방법(NAMA_Ur)급상규전자현미경기술，관찰료양총세포핵인FC(섬유중심)내DNA적초미결구，발현FC내DNA존재착일개개우집축도해집축지간적변화과정，병게시료DNA재핵인내적련속배포과정，즉핵인외DNA경과핵인통도진입도FC후，계속연FC적변연혹DFC(치밀섬유성분)여FC적교계처배요FC이배포，재경FC지간적핵인통도，연신도령외적FC구역。
By using the DNA specific cytochemical staining method (NAMA_Ur) and conventional electron microscopic technique, the authors examined the configuration of intranucleolar DNA in Allium cepa L. cells and found that nucleolar DNA within the fibrillar center (FC) underwent a structural transformation process from condensed to extended state. The authors' observations also displayed a continuous arrangement process of nucleolar DNA, i.e., the extranucleolar DNA entered FC through the nucleolar organizer region (NOR) channel, then extended to the periphery of FC or to the border between FC and dense fibrillar component (DFC), and distributed along the periphery of FC. Thence, by passing through the NOR channel between FCs, the nucleolar DNA continued to transfer to other FCs and arranged in the same above_mentioned forms.