CAJ | 학술논문

本研究对番木瓜不同材料及其深加工制品的核酸提取方法进行了优化,成功提取出可用于PCR扩增反应的DNA.选取番木瓜特异性的内源蛋白PAPAIN基因片段作为内参基因,用于检验提取DNA的质量,有效避免了PCR反应的假阴性并能用于确定检测样品中是否含有番木瓜成分.选取国内目前应用比较多的外源目的基因环斑病毒外壳蛋白(CP)基因以及复制酶(RP)基因作为品系鉴定的基因.本方法适用于出入境检验检疫部门和农业部门对转基因番木瓜及其制品的检验监测.
본연구대번목과불동재료급기심가공제품적핵산제취방법진행료우화,성공제취출가용우PCR확증반응적DNA.선취번목과특이성적내원단백PAPAIN기인편단작위내삼기인,용우검험제취DNA적질량,유효피면료PCR반응적가음성병능용우학정검측양품중시부함유번목과성분.선취국내목전응용비교다적외원목적기인배반병독외각단백(CP)기인이급복제매(RP)기인작위품계감정적기인.본방법괄용우출입경검험검역부문화농업부문대전기인번목과급기제품적검험감측.
This research has optimized the method of extracting the DNA of different parts of papaya and its highly-processed products, therefore successfully extracted the DNA for PCR(polymerase chain reation). Papain gene,a papaya species-specific protein gene is adopted as an internal control to access the quality of the DNA extracted so as to avoid the pseudo-negative case of PCR reaction and to confirm the existence of papaya in the samples as well. In this research, the genes applied are PRSV (papaya-ring-spot-virus) CP(coat protein) gene and RP(replicase) gene. This method is applicable and reliable for the determination and supervision of GM papaya and its processed products for Bureau of Entry-Exit Inspection and Bureau of Agriculture.