CAJ | 학술논문

目的通过德赛公司尿微量清蛋白(UmAlb)免疫比浊法试剂盒分析性能的测定,评价UmAlb测定方法的临床应用价值.方法采用全自动生化分析仪OLYMPUS AU2700对该试剂盒精密度、准确度、特异性等指标进行评价.结果批内和批间变异系数分别为1.56%～4.70%和2.10%～10.12%,平均回收率为109.64%,低于27.05 mmoL/L的葡萄糖溶液和低于0.05 g/L的维生素C(VitC)对UmAlb检测无显著干扰.UmAlb在0～300 mg/L内线性良好.结论目前使用的UmAlb免疫比浊试剂盒重复性尚可,准确性欠佳,线性范围良好,不受低于27.05 mmoL/L的葡萄糖溶液和低于0.05 g/L的VitC的干扰.建议购买mAlb检测质控液进行室内质控,以提高实验室检测结果的准确性和重复性,满足临床需要.
목적 통과덕새공사뇨미량청단백(UmAlb)면역비탁법시제합분석성능적측정,평개UmAlb측정방법적림상응용개치.방법 채용전자동생화분석의OLYMPUS AU2700대해시제합정밀도、준학도、특이성등지표진행평개.결과 비내화비간변이계수분별위1.56%～4.70%화2.10%～10.12%,평균회수솔위109.64%,저우27.05 mmoL/L적포도당용액화저우0.05 g/L적유생소C(VitC)대UmAlb검측무현저간우.UmAlb재0～300 mg/L내선성량호.결론 목전사용적UmAlb면역비탁시제합중복성상가,준학성흠가,선성범위량호,불수저우27.05 mmoL/L적포도당용액화저우0.05 g/L적VitC적간우.건의구매mAlb검측질공액진행실내질공,이제고실험실검측결과 적준학성화중복성,만족림상수요.
Objective To evaluate the reliability of Urine micro albumin immunoturbidimetry determination kit.Methods Comfirm the kit pricision,accuracy,specificity and interference by full automatic biochemistry analyzer OLYMPUS AU2700 .Results The chemical characters evaluation showed that the intraassay coefficient of variation is from 2.75 to 4.7%;the intraassay coefficient of variation is from2.1 to 10.12%;and the average recovery rate is 109.64%.The assay have no significant interferences by glucose solution concentration less than or equal to 27.05 mmoL/L and Vitamin C strength less than or equal to 0.05g, and there are good linearity in 0-300 mg/L .Conclusion The Pricision of the result of kit of UmAlb determined by this method illustrated above is reliable, but the accuracy is not reliable. Advocate to buy a quality control in order to increase the pricise result in the clinical laboratory.