南昌大学学报(理科版)
南昌大學學報(理科版)
남창대학학보(이과판)
JOURNAL OF NANCHANG UNIVERSITY(NATURAL SCIENCE)
2009年
5期
456-459,464
,共5页
荧光光谱%N-(对二甲氨基苯甲酰胺)-N′-苯基脲%血清白蛋白
熒光光譜%N-(對二甲氨基苯甲酰胺)-N′-苯基脲%血清白蛋白
형광광보%N-(대이갑안기분갑선알)-N′-분기뇨%혈청백단백
serum albumin%1-[p-(dimethylamino)benzoyl]-4-phenyl-semicarbazide%fluorescence spectroscopy
合成了主体化合物N-(对二甲氨基苯甲酰胺)-N′-苯基脲(1),采用荧光光谱法研究1与血清白蛋白间的相互作用.在pH 7.40的Tris-HCl缓冲溶液中,主体1的荧光强度在一定范围内随牛血清白蛋白(BSA)或人血清白蛋白(HSA)的加入呈线性增强.同时主体1可猝灭血清白蛋白的内源荧光,猝灭过程源于共振能量转移,并计算了主体1和血清白蛋白间的结合常数,分别为4 169 mol~(-1) L(BSA)和1 412 mol~(-1) L(HSA),结合位点数均近似为1.此外,同步荧光光谱的变化表明主体1与白蛋白作用时更接近其中的酪氨酸残基但未改变其构象.同时还研究了与1具有相似结构的N-(对二甲氨基苯甲酰胺)-N′-苯基硫脲(2)与血清白蛋白的相互作用,结果显示两者之间无明显的相互作用.蛋白质对两者光谱响应的差异可能是因为1具有两个类似于肽键的酰胺键,与蛋白质形成氢键,故与蛋白质的作用较强.
閤成瞭主體化閤物N-(對二甲氨基苯甲酰胺)-N′-苯基脲(1),採用熒光光譜法研究1與血清白蛋白間的相互作用.在pH 7.40的Tris-HCl緩遲溶液中,主體1的熒光彊度在一定範圍內隨牛血清白蛋白(BSA)或人血清白蛋白(HSA)的加入呈線性增彊.同時主體1可猝滅血清白蛋白的內源熒光,猝滅過程源于共振能量轉移,併計算瞭主體1和血清白蛋白間的結閤常數,分彆為4 169 mol~(-1) L(BSA)和1 412 mol~(-1) L(HSA),結閤位點數均近似為1.此外,同步熒光光譜的變化錶明主體1與白蛋白作用時更接近其中的酪氨痠殘基但未改變其構象.同時還研究瞭與1具有相似結構的N-(對二甲氨基苯甲酰胺)-N′-苯基硫脲(2)與血清白蛋白的相互作用,結果顯示兩者之間無明顯的相互作用.蛋白質對兩者光譜響應的差異可能是因為1具有兩箇類似于肽鍵的酰胺鍵,與蛋白質形成氫鍵,故與蛋白質的作用較彊.
합성료주체화합물N-(대이갑안기분갑선알)-N′-분기뇨(1),채용형광광보법연구1여혈청백단백간적상호작용.재pH 7.40적Tris-HCl완충용액중,주체1적형광강도재일정범위내수우혈청백단백(BSA)혹인혈청백단백(HSA)적가입정선성증강.동시주체1가졸멸혈청백단백적내원형광,졸멸과정원우공진능량전이,병계산료주체1화혈청백단백간적결합상수,분별위4 169 mol~(-1) L(BSA)화1 412 mol~(-1) L(HSA),결합위점수균근사위1.차외,동보형광광보적변화표명주체1여백단백작용시경접근기중적락안산잔기단미개변기구상.동시환연구료여1구유상사결구적N-(대이갑안기분갑선알)-N′-분기류뇨(2)여혈청백단백적상호작용,결과현시량자지간무명현적상호작용.단백질대량자광보향응적차이가능시인위1구유량개유사우태건적선알건,여단백질형성경건,고여단백질적작용교강.
A new compound,1-[p-(dimethylamino)benzoyl]-4-phenyl-semicarbazide (1),had been synthesized and the interactions between 1 and bovine serum albumin(BSA) or human serum albumin(HSA) were studied by fluorescence spectroscopy.The results suggested that the fluorescence intensity of 1 increased and showed good linear relationship with the amount of BSA or HSA.1 could also quench the intrinsic fluorescence of serum albumin through static quenching procedure.The binding constants (K_b) between 1 and proteins were 4169 mol~(-1) L for BSA and 1 412 mol~(-1) L for HSA respectively,meanwhile the average number of binding sites (n) was about 1.The binding distance between 1 and BSA or HSA was evaluated on the basis of the theory of energy transfer,which indicated that BSA could transfer energy to 1 more easily,compared with HSA.In addition,the effect of 1 on the conformation of BSA/HSA was investigated by synchronous fluorescence spectroscopy which implied that 1 was close to tyrosine residue but didn't effect the conformation of protein.At the same time,the interaction between the control compound [1-[p-(dimethylamino)benzoyl]-4-phenyl-thiosemicar bazide (2)] and proteins was also investigated.The results showed neither fluorescence spectral profile nor fluorescence intensity changes of 2 were observable in the presence of HSA or BSA.Thus,it was assumed that 1 possessed strong binding ability with protein due to the amide bond of urea group which is similar to the peptide bond.The results provide the useful data for drug design and screening.