研究采用3种不同的处理方式(I&GD、I&nGD和A&nGD)对雌鳗处理后,进行了生物学相关参数、肌肉脂肪酸组成和含量变化测定分析.研究结果表明,日本鳗鲡在性腺发育期间,体重一直呈一个下降的趋势,直到产卵前,才显著高于野生海鳗.该现象被称为水合反应.实验结束时,3个实验组的脂肪总量均有不同程度的下降,其中,I&GD组下降最多,占总脂肪的66.55%;其次是A&nGD组,占总脂肪的43.76%;下降最少的是I&nGD组,占总脂肪的34.68%.研究对日本鳗鲡的肌肉共测出了17种脂肪酸,SFA 7种、MUFA 2种、PUFA 8种.其中,MUFA虽然只有2种,但在脂肪酸总量中占的百分比却较高达38.02%,而且它们的消耗与性腺发育和基础代谢有关,与雌鳗的游泳耗能无关.在8种PUFA中,AA的变化很特殊.实验期间,它在日本鳗鲡肌肉内是一个积累和消耗的动态过程.而且,积累程度与雌鳗的游动有关,游动越多,积累越多.EPA虽然能被检出,但量很低,而且各组间的变化无明显规律.DHA在日本鳗鲡肌肉中的含量达到123.91 mg/g干肌肉,占总脂肪的32.52%.卵巢发育所消耗的量也很多,I&GD组在卵巢发育结束后,下降到10.59 mg/g十肌肉.相关性分析发现.虽然总脂肪酸、PUFA、DHA和EPA与GSI间均存在一定程度的负相关性,但关系最密切的是DHA,其r=-0.952(P<0.01,n=5),关系最小的是EPA,其r=-0.882(P<0.05,n=5).因此分析认为DHA是日本鳗鲡性腺发育所需的最主要的一种脂肪酸.对能量分布进行综合分析后认为,日本鳗鲡在人工诱导性腺发育期间,基础代谢所需的脂肪量(L_(BM))占总脂肪量的34.68%,性腺发育所需脂肪量(L_(GD))占总脂肪量的32.49%,运动耗能所需脂肪量(L_M)占总脂肪量的9.79%.因此认为,日本鳗鲡在不摄食的情况下,肌肉中的脂肪不仅能提供性腺发育所需的营养物质,而且还能满足洄游2000海里到达产卵场所需的能量.
研究採用3種不同的處理方式(I&GD、I&nGD和A&nGD)對雌鰻處理後,進行瞭生物學相關參數、肌肉脂肪痠組成和含量變化測定分析.研究結果錶明,日本鰻鱺在性腺髮育期間,體重一直呈一箇下降的趨勢,直到產卵前,纔顯著高于野生海鰻.該現象被稱為水閤反應.實驗結束時,3箇實驗組的脂肪總量均有不同程度的下降,其中,I&GD組下降最多,佔總脂肪的66.55%;其次是A&nGD組,佔總脂肪的43.76%;下降最少的是I&nGD組,佔總脂肪的34.68%.研究對日本鰻鱺的肌肉共測齣瞭17種脂肪痠,SFA 7種、MUFA 2種、PUFA 8種.其中,MUFA雖然隻有2種,但在脂肪痠總量中佔的百分比卻較高達38.02%,而且它們的消耗與性腺髮育和基礎代謝有關,與雌鰻的遊泳耗能無關.在8種PUFA中,AA的變化很特殊.實驗期間,它在日本鰻鱺肌肉內是一箇積纍和消耗的動態過程.而且,積纍程度與雌鰻的遊動有關,遊動越多,積纍越多.EPA雖然能被檢齣,但量很低,而且各組間的變化無明顯規律.DHA在日本鰻鱺肌肉中的含量達到123.91 mg/g榦肌肉,佔總脂肪的32.52%.卵巢髮育所消耗的量也很多,I&GD組在卵巢髮育結束後,下降到10.59 mg/g十肌肉.相關性分析髮現.雖然總脂肪痠、PUFA、DHA和EPA與GSI間均存在一定程度的負相關性,但關繫最密切的是DHA,其r=-0.952(P<0.01,n=5),關繫最小的是EPA,其r=-0.882(P<0.05,n=5).因此分析認為DHA是日本鰻鱺性腺髮育所需的最主要的一種脂肪痠.對能量分佈進行綜閤分析後認為,日本鰻鱺在人工誘導性腺髮育期間,基礎代謝所需的脂肪量(L_(BM))佔總脂肪量的34.68%,性腺髮育所需脂肪量(L_(GD))佔總脂肪量的32.49%,運動耗能所需脂肪量(L_M)佔總脂肪量的9.79%.因此認為,日本鰻鱺在不攝食的情況下,肌肉中的脂肪不僅能提供性腺髮育所需的營養物質,而且還能滿足洄遊2000海裏到達產卵場所需的能量.
연구채용3충불동적처리방식(I&GD、I&nGD화A&nGD)대자만처리후,진행료생물학상관삼수、기육지방산조성화함량변화측정분석.연구결과표명,일본만려재성선발육기간,체중일직정일개하강적추세,직도산란전,재현저고우야생해만.해현상피칭위수합반응.실험결속시,3개실험조적지방총량균유불동정도적하강,기중,I&GD조하강최다,점총지방적66.55%;기차시A&nGD조,점총지방적43.76%;하강최소적시I&nGD조,점총지방적34.68%.연구대일본만려적기육공측출료17충지방산,SFA 7충、MUFA 2충、PUFA 8충.기중,MUFA수연지유2충,단재지방산총량중점적백분비각교고체38.02%,이차타문적소모여성선발육화기출대사유관,여자만적유영모능무관.재8충PUFA중,AA적변화흔특수.실험기간,타재일본만려기육내시일개적루화소모적동태과정.이차,적루정도여자만적유동유관,유동월다,적루월다.EPA수연능피검출,단량흔저,이차각조간적변화무명현규률.DHA재일본만려기육중적함량체도123.91 mg/g간기육,점총지방적32.52%.란소발육소소모적량야흔다,I&GD조재란소발육결속후,하강도10.59 mg/g십기육.상관성분석발현.수연총지방산、PUFA、DHA화EPA여GSI간균존재일정정도적부상관성,단관계최밀절적시DHA,기r=-0.952(P<0.01,n=5),관계최소적시EPA,기r=-0.882(P<0.05,n=5).인차분석인위DHA시일본만려성선발육소수적최주요적일충지방산.대능량분포진행종합분석후인위,일본만려재인공유도성선발육기간,기출대사소수적지방량(L_(BM))점총지방량적34.68%,성선발육소수지방량(L_(GD))점총지방량적32.49%,운동모능소수지방량(L_M)점총지방량적9.79%.인차인위,일본만려재불섭식적정황하,기육중적지방불부능제공성선발육소수적영양물질,이차환능만족회유2000해리도체산란장소수적능량.
This experiment adopted three methods (I&GD,I&nGD and A&nGD) to treat the females of Japanese eel. After being treated, the biological data of female, which related with their gonadal development, were investigated and the changes of fatty acids in their muscle were measured by gas liquid chromatography. The purpose of this study is try to discuss the lipid consumptions for gonadal development, basal metabolization and migration fuel during spawning migration, as well as the relationship between gonadal development and fatty acids consumption in the period of artificial inducing gonadal maturation, in order to provide referenced foundation for future studies on artificial propagation in Japanese eel. The results indicated that the body weight of females came through a course of gradual downward trend before the 10 injection and rapid rise at the beginning of spawning, which was called hydration, as well as the lipids of muscle tissue were necessary not only for gonadal development, but also for basal metabolization and migration energies in Japanese eels during the spawning migration. In this study, 17 fatty acids were detected in muscle tissues of females, including 7 SFA, 2 MUFA and 8 PUFA. Among them, there were only two kinds of MUFA, but their total levels were higher and reached at 38.02 percent of total lipids. Their consumptions were related with gonadal development and basal metabolization, unrelated to the energy for swimming. Among 8 PUFA, the changes of AA in three treated groups were very special. It was a dynamic course of accumulation and consumption in muscle tissue of Japanese eel during the period of experiment and the accumulation was related with the swimming of females. The more the females swam, the more AA was accumulated in muscle tissues. DHA levels of muscle tissues in Japanese eels were the highest, its change was remarkably different between treated groups and its level in control reached up to (123.91+7.29) mg/g DMW, accounting for 32.52 percent of total lipids. However, its level in I&GD group dropped at (10.59+0.85) mg/g DMW at the end of experiment. Relativity analyses indicated that the relativities between GSI and the levels of total lipids, PUFA, DHA and EPA were all showed in some extent negative relativities, but the closest factor was DHA, r = -0.952(P<0.01 ,n =5) , the most distant one was EPA, r =-0.882 (P<0.05,n=5). Therefore, it was indicated that DHA was the most important fatty acid during the period of gonadal development in Japanese eel. Analyzing for energy distribution considered that the lipid consumption for basal metabolization (L_(BM)) accounted for 34.68% of total lipids, gonadal development (L_(GD)) accounted for 32.94% and swimming energy (L_(SE)) accounted for 9.79%. After finished spawning, the muscle of females was also reserved 23.04% of total lipids. Therefore, it can be undoubted that Japanese eels not only can provide enough energy to finish the spawning migration so far as over 2000 sea miles but also make the gonadal normal development without intake any food.
