CAJ | 학술논문

采用红外光谱法(FTIR)、二阶导数红外光谱法和二维相关光谱分析技术(Two-Dimensional Corre-lation Spectroscopy)对江西和安徽大血藤药材、水提物、蒽醌类提取物和残渣进行了快速无损比对分析研究,从中发现两产地大血藤药材的谱图中均出现1400～1 620 cm~(-1)和1 000～1 200 cm~(-1)两个强峰带,说明药材含有大量的药效成分总醌类和糖类物质;蒽醌提取物的谱图中也有上述2个谱带,但1 400～1 620cm~(-1)谱带明显强于1 000～1 200 cm~(-1)谱峰,说明该提取上艺适宜提取蒽醌类物质;而水提物中2个谱峰带则是后者明显强于前者,证实该提取工艺适宜提取糖类物质;另外通过红外谱图和二阶导数红外谱图的对比发现在药材残渣中含有大量的草酸钙品体.证明红外光谱技术不仅能对原药材及提取物的药效基团进行分析和指认,还能区分不同产地药材的提取物含最的高低,成功地建立了大血藤及其不间提取物药效成分比对分析的新方法.
채용홍외광보법(FTIR)、이계도수홍외광보법화이유상관광보분석기술(Two-Dimensional Corre-lation Spectroscopy)대강서화안휘대혈등약재、수제물、은곤류제취물화잔사진행료쾌속무손비대분석연구,종중발현량산지대혈등약재적보도중균출현1400～1 620 cm~(-1)화1 000～1 200 cm~(-1)량개강봉대,설명약재함유대량적약효성분총곤류화당류물질;은곤제취물적보도중야유상술2개보대,단1 400～1 620cm~(-1)보대명현강우1 000～1 200 cm~(-1)보봉,설명해제취상예괄의제취은곤류물질;이수제물중2개보봉대칙시후자명현강우전자,증실해제취공예괄의제취당류물질;령외통과홍외보도화이계도수홍외보도적대비발현재약재잔사중함유대량적초산개품체.증명홍외광보기술불부능대원약재급제취물적약효기단진행분석화지인,환능구분불동산지약재적제취물함최적고저,성공지건립료대혈등급기불간제취물약효성분비대분석적신방법.
The present study is to compare and analyze Sargentodoxa cuneata (oliv) Rehd. Etwils herbs, the aqueous, anthra-quinone extracts and residue in Jiang Xi and An Hui quickly and undamagedly by Fourier transform infrared spectroscopy(FTIR) and two-dimensional correlation spectroscopy. In the spectra of DaXueTeng, there are two strong peak areas at about 1 400-1 620 cm~(-1) and 1 000-1 200 cm~(-1) and it was concluded that DaXueTeng contains much glycoside and anthraquinone. Anthra-quinone extracts only exhibit strong peaks of 1 400-1 620 cm~(-1) which were stronger than that of 1 000-1 200 cm~(-1), and this illustrated that this method was adapted to extracting anthraquinone; then aqueous only show powerful peaks of 1 000-1 200 cm~(-1), so the authors know that the craftwork was suitable for extracting glycoside; finally, the authors also found that the residue of DaXueTeng contained much calcium oxalate. All of these illustrated that FTIR could not only analyze and identify traditional Chinese medicine (TCM) and extract components, but also discriminate contents of different extracts of TCM. The authors developed the new method to analyze and evaluate the DaXueTeng and their pharmacodynamic extracts successfully.