中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2010年
1期
72-75,80
,共5页
细胞壁结合蛋白%猪链球菌2型%生物信息学%毒力因子
細胞壁結閤蛋白%豬鏈毬菌2型%生物信息學%毒力因子
세포벽결합단백%저련구균2형%생물신식학%독력인자
cell wall-binding protein%Streptococcus suis type 2%bioinformatics%virulence factors
目的 为预测已公布基因组序列的2型猪链球菌(SS2)98HAH33株的细胞壁结合蛋白,以研究它们在SS2致病过程中的作用.方法 首先从GenBank获取由SS2 8HAH33基因组推测蛋白质氨基酸序列, 然后根据分选酶底物的结构特征,采用"三部分模式"法鉴别分选酶底物;采用手动方法寻找在具有I型信号肽的蛋白质中具有LysM域、WxL域、胆碱结合域、GW域或S层同源域的蛋白质;利用InterProScan和BlastP服务器,对鉴定的蛋白质进行功能分析.结果 共预测出9种分选酶底物,2种具有LysM域的蛋白. YP_001201232、YP_001201531和YP_001201656等3种已证实位于SS2菌体表面;其中YP_001201232为已知毒力因子OSF.另外4种蛋白质的功能分析表明,YP_001201484为糖苷酶,YP_001201544为枯草杆菌素样丝氨酸酶,YP_001199825和YP_001199755可能结合H因子,可能为新的毒力因子. YP_001200959、和YP_001201233 等2种功能未知.结论 提示SS2的多数分选酶底物可能为SS2的毒力因子,与致病过程相关.
目的 為預測已公佈基因組序列的2型豬鏈毬菌(SS2)98HAH33株的細胞壁結閤蛋白,以研究它們在SS2緻病過程中的作用.方法 首先從GenBank穫取由SS2 8HAH33基因組推測蛋白質氨基痠序列, 然後根據分選酶底物的結構特徵,採用"三部分模式"法鑒彆分選酶底物;採用手動方法尋找在具有I型信號肽的蛋白質中具有LysM域、WxL域、膽堿結閤域、GW域或S層同源域的蛋白質;利用InterProScan和BlastP服務器,對鑒定的蛋白質進行功能分析.結果 共預測齣9種分選酶底物,2種具有LysM域的蛋白. YP_001201232、YP_001201531和YP_001201656等3種已證實位于SS2菌體錶麵;其中YP_001201232為已知毒力因子OSF.另外4種蛋白質的功能分析錶明,YP_001201484為糖苷酶,YP_001201544為枯草桿菌素樣絲氨痠酶,YP_001199825和YP_001199755可能結閤H因子,可能為新的毒力因子. YP_001200959、和YP_001201233 等2種功能未知.結論 提示SS2的多數分選酶底物可能為SS2的毒力因子,與緻病過程相關.
목적 위예측이공포기인조서렬적2형저련구균(SS2)98HAH33주적세포벽결합단백,이연구타문재SS2치병과정중적작용.방법 수선종GenBank획취유SS2 8HAH33기인조추측단백질안기산서렬, 연후근거분선매저물적결구특정,채용"삼부분모식"법감별분선매저물;채용수동방법심조재구유I형신호태적단백질중구유LysM역、WxL역、담감결합역、GW역혹S층동원역적단백질;이용InterProScan화BlastP복무기,대감정적단백질진행공능분석.결과 공예측출9충분선매저물,2충구유LysM역적단백. YP_001201232、YP_001201531화YP_001201656등3충이증실위우SS2균체표면;기중YP_001201232위이지독력인자OSF.령외4충단백질적공능분석표명,YP_001201484위당감매,YP_001201544위고초간균소양사안산매,YP_001199825화YP_001199755가능결합H인자,가능위신적독력인자. YP_001200959、화YP_001201233 등2충공능미지.결론 제시SS2적다수분선매저물가능위SS2적독력인자,여치병과정상관.
To identify the cell wall-binding proteins of Streptococcus suis serotype 2(SS2), according to their structure feature, the substrates of sortase and I type secreted proteins with LysM domain, WxL domain, choline-binding domain,GW domain or S layer homologous domain in the recently published genome of SS2 strain 98HAH33 were firstly identified and then the putative functions were attributed to individual proteins by reference to the identification of conserved domains of InterPro and BlastP servers. Homologous proteins were identified by unfiltered BlastP homology searches (including conserved domain detection). Among the 23 putative proteins with a C-terminal LPXTG recognition signal for covalent attachment to peptidoglycan by sortase, 9 with I signal peptide were identified as sortase substrates. Among 9 substrates , YP_001201232, YP_001201531 and YP_001201656 had been experimentally verified to anchor to bacterial cell wall , and YP_001201232 known as the opacity factor of S. suis (OFS) was proved to be the virulence factors. According to function analysis, YP_001201484, YP_001201544, YP_001199825, YP_001197640, YP_001197840 and YP_001199755 appeared to be involved in SS2 pathogenesis. and YP_001200959, YP_001201233 and two proteins with LysM domain( YP_001199784 and YP_001201729) were the hypothetical proteins. These data suggest the majority of putative sortase substrates may implicate in the virulence of SS2 and could serve as a basis for targeted experimental studies into the function of these proteins.
