CAJ | 학술논문

利用BTB平板培养基以及硝化-反硝化性能测定,从实验室驯化成熟的SBR反应器中筛选出3株异养硝化-好氧反硝化菌.其中WYLW1-6菌株的硝化-反硝化性能尤为突出,经16S rDNA基因序列分析和Biolog测定,该菌株属于蜡状芽孢杆菌(Bacillus cereus).在摇瓶培养时,对该菌株设计L_4(2~3)的正交试验,结果发现,菌株WYLW1-6投加到水中后启动迅速,且生长适应性强,优选条件下,其氨氮最大去除率可达95.21%.用发酵罐对其扩大培养测定N_2O逸出量,表明该菌脱氮效果良好,NH4_+~-N去除率达到97.19%,TN去除率为96.63%,N_2O逸出量为1.849 2 mg,其中N_2O-N量为1.176 8 mg,N_2O-N量占水中脱除TN量的0.598%.菌株WYLW1-6能够独立完成生物脱氮的全过程,高效脱氮的同时N_2O逸出量低.该菌可用于构建一个低N_2O逸出的高效脱氮菌系,从而实现N_2O生物控逸.
이용BTB평판배양기이급초화-반초화성능측정,종실험실순화성숙적SBR반응기중사선출3주이양초화-호양반초화균.기중WYLW1-6균주적초화-반초화성능우위돌출,경16S rDNA기인서렬분석화Biolog측정,해균주속우사상아포간균(Bacillus cereus).재요병배양시,대해균주설계L_4(2~3)적정교시험,결과발현,균주WYLW1-6투가도수중후계동신속,차생장괄응성강,우선조건하,기안담최대거제솔가체95.21%.용발효관대기확대배양측정N_2O일출량,표명해균탈담효과량호,NH4_+~-N거제솔체도97.19%,TN거제솔위96.63%,N_2O일출량위1.849 2 mg,기중N_2O-N량위1.176 8 mg,N_2O-N량점수중탈제TN량적0.598%.균주WYLW1-6능구독립완성생물탈담적전과정,고효탈담적동시N_2O일출량저.해균가용우구건일개저N_2O일출적고효탈담균계,종이실현N_2O생물공일.
By using BTB medium and measuring nitrification-denitrification characteristics,three heterotrophic nitrification-aerobic denitrification strains were isolated from a laboratory-matured SBR reactor.Among them,strain WYLW1-6 exhibited the highest nitrification-denitrification ability.Based on the analysis of the 16S rDNA gene sequence and the detection of Biolog,strain WYLW1-6 was identified as Bacillus cereus.To research the best heterotrophic nitrification-aerobic denitrification conditions of strain WYLW1-6,an orthogonal experiment of L_4(2~3) was designed in shake flask cultivation.The results showed that the maximum removal rate of ammonia nitrogen was 95.21%.Under the optimal conditions,strain WYLW1-6 could grow and adapt to the condition rapidly after inoculating into the water.To expand culture and measure the yield of N_2O,strain WYLW1-6 was cultured in fermentor; the removal rates of ammonia nitrogen and TN were 97.19% and 96.63% respectively,and total production of N_2O was 1.8492 mg,which accounted for 0.598% of the removed TN.Strain WYLW1-6 can accomplish the whole bio-denitrification process independently,removing nitrogen efficiently and yielding low N_2O.This strain can be used to build a complex strain community of low N_2O emission and high nitrogen removal,while realizing bio-control of N_2O emissions.