CAJ | 학술논문

目的:建立左金丸(黄连、吴茱萸)和香连丸(黄连(吴茱萸制)、木香)中6种生物碱含量的LC-MS/MS测定方法.方法:色谱条件:Phenomenex Lunac_(18)柱(5μm,250mm×4.60mm);乙腈-水(4mmol/L乙酸铵和0.08%甲酸)为流动相,梯度洗脱;质谱条件:采用电喷雾离子源(ESI),正离子检测,MRM模式.结果:左金丸中药根碱、黄连碱、巴马汀、小檗碱、吴茱萸碱和吴茱萸次碱的平均回收率分别为101.0%,101.3%、101.2%、100.2%、99.6%、99.7%,RSD分别为1.1%、1.8%、0.98%、1.0%、1.3%、1.3%,香连丸中药根碱、黄连碱、巴马汀、小檗碱和吴茱萸碱的平均回收率分别为101.6%、99.8%、101.9%、101.0%、101.3%,RSD分别为1.0%、1.9%、0.75%、1.1%、1.9%.结论:本方法可同时测定左金丸和香连丸中药根碱、黄连碱、巴马汀、小檗碱、吴茱萸碱和吴茱萸次碱的含量,具有快速、简便等特点.
목적:건립좌금환(황련、오수유)화향련환(황련(오수유제)、목향)중6충생물감함량적LC-MS/MS측정방법.방법:색보조건:Phenomenex Lunac_(18)주(5μm,250mm×4.60mm);을정-수(4mmol/L을산안화0.08%갑산)위류동상,제도세탈;질보조건:채용전분무리자원(ESI),정리자검측,MRM모식.결과:좌금환중약근감、황련감、파마정、소벽감、오수유감화오수유차감적평균회수솔분별위101.0%,101.3%、101.2%、100.2%、99.6%、99.7%,RSD분별위1.1%、1.8%、0.98%、1.0%、1.3%、1.3%,향련환중약근감、황련감、파마정、소벽감화오수유감적평균회수솔분별위101.6%、99.8%、101.9%、101.0%、101.3%,RSD분별위1.0%、1.9%、0.75%、1.1%、1.9%.결론:본방법가동시측정좌금환화향련환중약근감、황련감、파마정、소벽감、오수유감화오수유차감적함량,구유쾌속、간편등특점.
AIM:To simultaneously determine contents of jatrorrhizine,coptisine,palmatine;berberinee,evodiamine and rutaecarpine in Zuojin Pill(Rhizoma Coptidis,Fructus Evodiae)Xianglian Pill(Rhizoma Coptidis(processed with Fructus Evodiae),Radix Aucklandiae).METHODS:Chromatographic separation was achieved with gradient elution by a C_(18) column hyphenated to an API 4000 mass spectrometer system was operated under the MRM mode with eleetrospray positive ionization(ESI).The mobile phase A consisted of acetonitrile-water with 4 mmol/L ammonium acetate and 0.08%methanoic acid.RESULTS:The average recoveries of jatrorrhizine,coptisine,palmatine,berberinee,evodiamine and rutaecarpine in Zuojin Pill were 101.0%,101.3%,101.2%,100.3%,99.6%,99.7%,and the RSD were 1.1%,1.8%,0.98%,1.O%,1.3%,1.3%,respectively.The average recoveries of jatrorrhizine,coptisine,palmatine,berberinee and evodiamine in Xianglian Pill were 101.6%,99.8%,101.9%,101.0%,101.3%,and the RSD were 1.0%,1.9%,0.75%,1.1%,1.9%,respectively.CONCLUSIoN:This method is fast,sensitive and reproducible.It can be used to determine jatrorrhizine,coptisine,palmatine,berberinee,evodiamine and rutaecarpine in Zuojin Pill and Xianglian Pill under the sanle chromatogram condition.