CAJ | 학술논문

目的动态分析微囊化肝细胞移植对急性肝功能衰竭(ALF)大鼠肝组织中血管内皮生长因子(VEGF)表达的影响.方法 D-氨基半乳糖诱导建立ALF大鼠模型18 h后,分成ALF模型组、裸肝细胞移植组和微囊化肝细胞移植组各50只大鼠.造模后24、36、48、72、120、168和240 h取各组大鼠肝组织.免疫组织化学法检测VEGF和增殖细胞核抗原(PCNA)表达,半定量RT-PCR检测VEGF mRNA表达.多组样本组间比较采用单因素方差分析.结果与ALF模型组比较,裸肝细胞移植组和微囊化肝细胞移植组大鼠肝组织中PCNA表达量在36 h开始即显著增高(F=26.26,P<0.05).与ALF模型组和裸肝细胞移植组比较,微囊化肝细胞移植组大鼠肝组织中VEGF表达量从36 h开始明显升高(F=25.44,P<0.05),且下降也较缓慢,在240 h表达量仍显著高于ALF模型组和裸肝细胞移植组(F=220.25,P<0.05).造模24 h,裸肝细胞移植组VEGFmRNA(56.7±9.1)开始高于ALF模型组(48.0±1.9),差异有统计学意义(F=3.54,P<0.05),而微囊化肝细胞移植组(100.7±1.9)在48 h开始显著高于裸肝细胞移植组(94.5±1.4),差异有统计学意义(F=47.82,P<0.05),至240 h时,表达量仍显著高于裸肝细胞移植组,差异有统计学意义(F=21.70,P<0.05).结论微囊化肝细胞移植可增加ALF大鼠肝组织中VEGF的表达,促进肝细胞的再生.
목적 동태분석미낭화간세포이식대급성간공능쇠갈(ALF)대서간조직중혈관내피생장인자(VEGF)표체적영향.방법 D-안기반유당유도건립ALF대서모형18 h후,분성ALF모형조、라간세포이식조화미낭화간세포이식조각50지대서.조모후24、36、48、72、120、168화240 h취각조대서간조직.면역조직화학법검측VEGF화증식세포핵항원(PCNA)표체,반정량RT-PCR검측VEGF mRNA표체.다조양본조간비교채용단인소방차분석.결과 여ALF모형조비교,라간세포이식조화미낭화간세포이식조대서간조직중PCNA표체량재36 h개시즉현저증고(F=26.26,P<0.05).여ALF모형조화라간세포이식조비교,미낭화간세포이식조대서간조직중VEGF표체량종36 h개시명현승고(F=25.44,P<0.05),차하강야교완만,재240 h표체량잉현저고우ALF모형조화라간세포이식조(F=220.25,P<0.05).조모24 h,라간세포이식조VEGFmRNA(56.7±9.1)개시고우ALF모형조(48.0±1.9),차이유통계학의의(F=3.54,P<0.05),이미낭화간세포이식조(100.7±1.9)재48 h개시현저고우라간세포이식조(94.5±1.4),차이유통계학의의(F=47.82,P<0.05),지240 h시,표체량잉현저고우라간세포이식조,차이유통계학의의(F=21.70,P<0.05).결론 미낭화간세포이식가증가ALF대서간조직중VEGF적표체,촉진간세포적재생.
Objective To study the expression of vascular endothelial growth factor(VEGF)in hepatic tissue of acute liver failure(ALF)rats after transplantation of microencapsulated hepatocytes.Methods The ALF model of rats was induced by D-galactosamine(D-Gal)1400 mg/kg.Then the ALF rats were divided into three groups 18 h after injection of D-Gal:ALF model group,free hepatocyte transplantation group,and microencapsulated hepatocyte transplantation group,50 rats in each group,respectively.Each group was divided into 7 subgroups,and the rats were sacrificed at 24,36,48,72,120,168 and 240 h respectively after injection of D-Gal.The expression of proliferating cell nuclear antigen(PCNA) and VEGF in liver tissue of ALF rats was detected by immunohistochemistry method.VEGF mRNA in liver tissue was detected by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR).Comparison among groups was done by one way ANOVA.Results Compared with ALF model group,the expression of PCNA protein and in free hepatocyet transplantation group in microencapsulated hepatocyte transplantation group began to increase obviously at 36 h after injection of D-Gal(F=26.26,P<0.05).Compare to that of ALF model and free hepatocyte transplantation group,the expression of VEGF protein in microencapsulated hepatocyte transplantation group increased significantly at 36 h after injection of D-Gal(F=25.44,P<0.05),which decreased slower and was still higher at 240 h after injection of D-Gal(F=220.25,P<0.05).Compared with ALF model group,the expression of VEGF mRNA in free hepatocyte transplantation group increased obviously at 24 h(48.0±1.9 vs 56.7±9.1;F=3.54,P<0.05).And that in microencapsulated hepatocyte transplantation group was higher than free hepatocyte transplantation group at 48 h(100.7±1.9 vs 94.5±1.4;F=47.82,P<0.05),which was still significantly higher than free hepatocyte transplantation group at 240 h after injection of D-Gal(F=21.70,P<0.05). Conclusion Transplantation of microencapsulated hepatocytes could upregulate the expression of VEGF in liver tissues and promote the regeneration of hepatocytes in ALF rats.