中国行为医学科学
中國行為醫學科學
중국행위의학과학
2008年
10期
872-874
,共3页
闫丽萍%马骋%易建良%徐恒泽
閆麗萍%馬騁%易建良%徐恆澤
염려평%마빙%역건량%서항택
电针%神经病理性痛%脊髓%一氧化氮合酶%一氧化氮
電針%神經病理性痛%脊髓%一氧化氮閤酶%一氧化氮
전침%신경병이성통%척수%일양화담합매%일양화담
Electroacupuncture%Neuropathic pain%Spinal cord%NOS%NO
目的 探讨电针对神经病理性痛大鼠痛觉过敏以及脊髓一氧化氮合酶(NOS)活性和一氧化氮(NO)水平的影响.方法 40只SD大鼠,随机分为空白组、假手术组、手术组和电针组(n=10).采用坐骨神经分支选择性损伤模型,电针"委中"和"环跳"穴,观察其对大鼠机械痛阈和热痛阈的影响,以分光光度法测定脊髓总NOS以及nNOS、iNOS活性和NO水平.结果 坐骨神经分支选择性损伤术可明显降低大鼠机械痛阈,手术组10d时为(14.83±0.79)g,与空白组和假手术组相比,差异有显著性(P＜0.05);并且手术组脊髓总NOS和nNOS活性以及NO水平均明显升高,分别为(44.23±7.81)U/mgprot和(39.64±10.28)U/mgprot以及(112.79±18.01)μmol/g,与空白组和假手术组相比,均差异有显著性(P＜0.01),iNOS却有降低的趋势;而电针干预后大鼠脊髓总NOS和nNOS活性明显降低,分别为(32.14±12.05)U/mgprot和(20.97±12.16)U/mgprot,NO合成也明显减少,为(70.96±18.15)μmoL/g,与手术组各项比较差异有显著性(P＜0.05,P＜0.01);与此同时显著减轻坐骨神经分支选择性损伤大鼠的机械痛敏状态,进而改善其痛行为.结论 电针减轻神经病理性痛可能与其抑制脊髓NOS活性、降低NO水平有关.
目的 探討電針對神經病理性痛大鼠痛覺過敏以及脊髓一氧化氮閤酶(NOS)活性和一氧化氮(NO)水平的影響.方法 40隻SD大鼠,隨機分為空白組、假手術組、手術組和電針組(n=10).採用坐骨神經分支選擇性損傷模型,電針"委中"和"環跳"穴,觀察其對大鼠機械痛閾和熱痛閾的影響,以分光光度法測定脊髓總NOS以及nNOS、iNOS活性和NO水平.結果 坐骨神經分支選擇性損傷術可明顯降低大鼠機械痛閾,手術組10d時為(14.83±0.79)g,與空白組和假手術組相比,差異有顯著性(P＜0.05);併且手術組脊髓總NOS和nNOS活性以及NO水平均明顯升高,分彆為(44.23±7.81)U/mgprot和(39.64±10.28)U/mgprot以及(112.79±18.01)μmol/g,與空白組和假手術組相比,均差異有顯著性(P＜0.01),iNOS卻有降低的趨勢;而電針榦預後大鼠脊髓總NOS和nNOS活性明顯降低,分彆為(32.14±12.05)U/mgprot和(20.97±12.16)U/mgprot,NO閤成也明顯減少,為(70.96±18.15)μmoL/g,與手術組各項比較差異有顯著性(P＜0.05,P＜0.01);與此同時顯著減輕坐骨神經分支選擇性損傷大鼠的機械痛敏狀態,進而改善其痛行為.結論 電針減輕神經病理性痛可能與其抑製脊髓NOS活性、降低NO水平有關.
목적 탐토전침대신경병이성통대서통각과민이급척수일양화담합매(NOS)활성화일양화담(NO)수평적영향.방법 40지SD대서,수궤분위공백조、가수술조、수술조화전침조(n=10).채용좌골신경분지선택성손상모형,전침"위중"화"배도"혈,관찰기대대서궤계통역화열통역적영향,이분광광도법측정척수총NOS이급nNOS、iNOS활성화NO수평.결과 좌골신경분지선택성손상술가명현강저대서궤계통역,수술조10d시위(14.83±0.79)g,여공백조화가수술조상비,차이유현저성(P＜0.05);병차수술조척수총NOS화nNOS활성이급NO수평균명현승고,분별위(44.23±7.81)U/mgprot화(39.64±10.28)U/mgprot이급(112.79±18.01)μmol/g,여공백조화가수술조상비,균차이유현저성(P＜0.01),iNOS각유강저적추세;이전침간예후대서척수총NOS화nNOS활성명현강저,분별위(32.14±12.05)U/mgprot화(20.97±12.16)U/mgprot,NO합성야명현감소,위(70.96±18.15)μmoL/g,여수술조각항비교차이유현저성(P＜0.05,P＜0.01);여차동시현저감경좌골신경분지선택성손상대서적궤계통민상태,진이개선기통행위.결론 전침감경신경병이성통가능여기억제척수NOS활성、강저NO수평유관.
Objective To investigate the effect of electroacupuncture(EA)on hyperalgesia,and NOS activity and NO concentration within the spinal cord of rats with spared nerve injury(SNI).Methods Forty adult SD rats were randomly divided into a control(C)group,a sham-operation(SO)group and all operation(O)group.A spared nerve injury(SNI)model was established by ligating and severing the common peroneal nerve and the proso-tibial nerve under anesthesia.The rats within the O group were randomly divided into a model(M)subgroup,and all electroacupuncture(E)subgroup.The Success of the SNI operation was measured by determining the mechanical pain thresholds of each rat.In the E subgroup EA(2Hz,onset 1mA,enhance 1mA each 10 min)was applied to"Wei zhong"(BL40)and"Huan tiao"(GB 30)for 30 minutes.Total NOS,nNOS,and iNOS activity as well as the concentration of NO were then measured by absorption spectrometry.Results After the SNI operation the mechanical pain threshold for the O group at day 10 (14.83±0.79g)was significantly lower then that of groups C and SO(P＜0.01 and P＜0.05 respectively).In addition,total NOS[(44.23±7.81)U/mgprot]and nNOS[(39.64±10.28)U/mgprot]activity as well as NO concentration[(112.79±18.01)]μmol/g]of the O group within the spinal cord were significantly(P＜0.01)higherthan those ofthe C and SM groups,whereas iNOS activity was markedly reduced.After acupuncture the total NOS[(32.14±12.05)U/mgprot,P＜0.05]and nNOS[(20.97±12.16)U/mgprot,P＜0.01]activity,as well as NO concentration[(70.96±18.15)μmol/g]within the spinal cord for the E subgroup was significantly reducedin comparison to that ofthe M subgroup;simultaneously,the mechanical pain thresholds of the E group heightened significantly compared to that of the M group.Conclusion It is suggested that EA analgesic mechanism may be related to the reduction of hyperalgesia,as well a decrease in NOS activity and NO concentration within the spinal cord in SNI.
