CAJ | 학술논문

目的研究miR-622在胃癌细胞中的表达,探讨miR-622在胃癌中的生物学功能.方法采用实时荧光定量PCR检测miR-622在胃癌细胞中的表达,以胃癌细胞SGC-7901和NCI-N87为模型瞬时转染miR-622寡核苷酸前体或抑制体,通过克隆形成、侵袭和划痕实验验证其在胃癌细胞中的功能.结果荧光定量PCR实验结果表明:miR-622在胃癌细胞SGC-7901中相对表达量为1.29±0.58,而在NCI-N87细胞中相对表达量为10.96±1.02.在SGC-7901细胞中转染了miR-622 寡核苷酸前体,克隆形成率为76％.划痕试验中愈合能力为(11±7)μm,胃癌细胞侵袭能力为(732±3)个,与对照组相比差异均有统计学意义(均P＜0.05).结论 miR-622能够促进胃癌细胞克隆形成、迁移和侵袭.
목적 연구miR-622재위암세포중적표체,탐토miR-622재위암중적생물학공능.방법 채용실시형광정량PCR검측miR-622재위암세포중적표체,이위암세포SGC-7901화NCI-N87위모형순시전염miR-622과핵감산전체혹억제체,통과극륭형성、침습화화흔실험험증기재위암세포중적공능.결과 형광정량PCR실험결과표명:miR-622재위암세포SGC-7901중상대표체량위1.29±0.58,이재NCI-N87세포중상대표체량위10.96±1.02.재SGC-7901세포중전염료miR-622 과핵감산전체,극륭형성솔위76％.화흔시험중유합능력위(11±7)μm,위암세포침습능력위(732±3)개,여대조조상비차이균유통계학의의(균P＜0.05).결론 miR-622능구촉진위암세포극륭형성、천이화침습.
Objective To investigate the biological function of miR-622 in human gastric cancer cell lines of SGC-7901 and NCI-N87 cells and its role in gastric carcinogenesis.Methods We analyzed the expression of miR-622 in those human gastric cancer cell lines by quantitative real-time polymerase chain reaction.Tumorigenesis,migration and invasion ability of miR-622 overexpression was assessed in vitro with miR-622 precursor and inhibitor in in SGC-7901 and NCI-N87 cells.Results The expression level of miR-622 in SGC 7901 was 1.29 ± 0.57,and it was 10.96 ± 1.02 in NCI-N87 cells.The soft agar colony formation rate was 76％ in SGC-7901 after transfecting miR-331-3p precursor,the ability of scratch healing was ( 11 ±7) μm,and the ability of transwell invasion was (731 ±3),compared with that in control group,the differences were statistically significant ( P ＜ 0.05 ).Conclusions Over-expression of miR-622 promotes tumorigenesis,migration,and invasion in gastric cancer cells in vitro.