CAJ | 학술논문

[目的]探讨皮肤疣组织中人乳头瘤病毒(HPV)的感染率和病毒型别.[方法]门诊皮肤疣患者132例中寻常疣46例,跖疣38例,扁平疣48例.以特异性DNA序列为模板设计引物,进行PCR扩增,电泳,对结果进行分析.[结果]132例皮肤疣中,HPV阳性128例,感染率为97.0％,其中HPV2型阳性111例,感染率为84.1％.寻常疣、跖疣感染主要为HPV2、H PV1型,扁平疣感染主要为HPV2、HPV 10、HPV3、HPV1型.89例为多重感染,感染率为67.4％,其中二重感染率为41.7％(55例),三重感染率为21.2％(28例),四重感染率为4.6％(6例)；寻常疣多重感染率为50.0％( 23/46),跖疣为76.3％( 29/38),扁平疣77.1％( 37/48).多重感染中,以HPV1+2型最多,有35例(39.3％),其次为HPV1+2+10型,有12例(13.5％).[结论]遵义皮肤疣感染以HPV2型为主,其次为1型；2型与多种皮肤疣感染有关；皮肤疣中多重感染率较高,其中扁平疣多重感染率最高；新发现HPV3、10型感染寻常疣,HPV10型感染跖疣,HPV1、7型感染扁平疣.
[목적]탐토피부우조직중인유두류병독(HPV)적감염솔화병독형별.[방법]문진피부우환자132례중심상우46례,척우38례,편평우48례.이특이성DNA서렬위모판설계인물,진행PCR확증,전영,대결과진행분석.[결과]132례피부우중,HPV양성128례,감염솔위97.0％,기중HPV2형양성111례,감염솔위84.1％.심상우、척우감염주요위HPV2、H PV1형,편평우감염주요위HPV2、HPV 10、HPV3、HPV1형.89례위다중감염,감염솔위67.4％,기중이중감염솔위41.7％(55례),삼중감염솔위21.2％(28례),사중감염솔위4.6％(6례)；심상우다중감염솔위50.0％( 23/46),척우위76.3％( 29/38),편평우77.1％( 37/48).다중감염중,이HPV1+2형최다,유35례(39.3％),기차위HPV1+2+10형,유12례(13.5％).[결론]준의피부우감염이HPV2형위주,기차위1형；2형여다충피부우감염유관；피부우중다중감염솔교고,기중편평우다중감염솔최고；신발현HPV3、10형감염심상우,HPV10형감염척우,HPV1、7형감염편평우.
[Objective] To detect and genotype HPV in cutaneous wart tissue.[Methods] This study recruited 132 outpatients with cutaneous warts in the Affiliated Hospital of Zunyi Medical College.Of the 132outpatients,46 had verruca vulgaris,38 had verruca plantaris,and 48 had verruca plana.Ten healthy human and 15 patients with condyloma acuminatum served as the control.Tissue specimens were obtained from the lesions of these patients as well as from the normal skin of the healthy controls.DNA was extracted from the specimens followed by PCR amplification with general and type-specific primers.[Results] Of the 132 wart specimens,128 (97.0％) were positive for HPV,111 (84.1％) for HPV2,89 (67.4％) for multiple types of HPV,55 (41.7％) for two types of HPV,28 (21.2％) for three types of HPV,and 6 (4.6％) for four types of HPV.The most prevalent HPV types were HPV2 followed by HPV1 in verruca vulgaris and verruca plantaris,HPV2 followed by HPV10,HPV3 and HPVl in verruca plana.Multiple types of HPV were identified in 50.0％(23/46) of the verruca vulgaris specimens,76.3％ (29/38) of the verruca plantaris specimens,and 77.1％(37/48) of the verruea plana specimens.Among patients infected with more than one type of HPV,the combination of HPV1 and 2 was the most common (35,39.3％),followed the combination of HPV1,2 and 10(12,13.5％).[Conclusion]s The most prevalent HPV type appears to be HPV2,followed by HPV1,in patients with cutaneous warts in Zunyi area of Guizhou province.HPV2 infection is associated with various types of cutaneous warts.There is a high incidence of coinfection with multiple types of HPV in patients with cutaneous warts,especially in those with verruca plana.Moreover,HPV3 and 10 are newly identified in verruca vulgaris,HPV 10 in verruca plantaris,HPV 1 and HPV7 in veruca plana.