中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2008年
3期
215-217
,共3页
籍会彩%林斌%向春艳%袁忠%温淑娟
籍會綵%林斌%嚮春豔%袁忠%溫淑娟
적회채%림빈%향춘염%원충%온숙연
呼吸道合胞病毒%酶联免疫吸附测定%抗原检测%流行病学
呼吸道閤胞病毒%酶聯免疫吸附測定%抗原檢測%流行病學
호흡도합포병독%매련면역흡부측정%항원검측%류행병학
Respiratory syncytial virus%Enzyme linked immunosorbent assay%Antigen detecting%Epidemiology
目的建它呼吸道合胞病毒抗原酶联免疫吸附测定(ELISA)检测方法,应用其对来源于本院的临床样本进行检测.方法建立ELISA方法,检测标本中的呼吸道合胞病毒,与呼吸道合胞病毒细胞培养检测方法进行对照,并利用该方法进行该病毒2007年在广州市的流行性分析.结果本方法的灵敏度略高于呼吸道合胞病毒的细胞培养检测方法,对甲型流感病毒(H3N2亚型、H1N1亚型)、乙型流感病毒、副流感病毒(Ⅰ型、Ⅲ型)、呼吸道腺病毒(Ⅲ型、Ⅶ型)无特异性反应.结论本呼吸道合胞病毒抗原ELISA检测方法可用于呼吸道合胞病毒感染的临床诊断和鉴别诊断.
目的建它呼吸道閤胞病毒抗原酶聯免疫吸附測定(ELISA)檢測方法,應用其對來源于本院的臨床樣本進行檢測.方法建立ELISA方法,檢測標本中的呼吸道閤胞病毒,與呼吸道閤胞病毒細胞培養檢測方法進行對照,併利用該方法進行該病毒2007年在廣州市的流行性分析.結果本方法的靈敏度略高于呼吸道閤胞病毒的細胞培養檢測方法,對甲型流感病毒(H3N2亞型、H1N1亞型)、乙型流感病毒、副流感病毒(Ⅰ型、Ⅲ型)、呼吸道腺病毒(Ⅲ型、Ⅶ型)無特異性反應.結論本呼吸道閤胞病毒抗原ELISA檢測方法可用于呼吸道閤胞病毒感染的臨床診斷和鑒彆診斷.
목적건타호흡도합포병독항원매련면역흡부측정(ELISA)검측방법,응용기대래원우본원적림상양본진행검측.방법건립ELISA방법,검측표본중적호흡도합포병독,여호흡도합포병독세포배양검측방법진행대조,병이용해방법진행해병독2007년재엄주시적류행성분석.결과본방법적령민도략고우호흡도합포병독적세포배양검측방법,대갑형류감병독(H3N2아형、H1N1아형)、을형류감병독、부류감병독(Ⅰ형、Ⅲ형)、호흡도선병독(Ⅲ형、Ⅶ형)무특이성반응.결론본호흡도합포병독항원ELISA검측방법가용우호흡도합포병독감염적림상진단화감별진단.
Objective To construct the method to detect respiratory syncytial virus(RSV) antigen by enzyme linked immunosorbent assay ( ELISA), and to detect the clinical samples from Nanfang Hospital in 2007. Methods The RSV antigen-detecting method by ELISA was constructed. Clinical samples were detected,and the results were compared to the cellular culture method. The epidemiology of RSV in Guangzhou was analyzed by the detecting results. Results The sensitivity of the antigen-detecting method was similar to the cellular culture of RSV. The method had no crossing reaction to H3N2 and H1N1 subtypes of influenza A virus, influenza B virus, 1 and 3 types of para-influenza virus, 3 and 7 types of adenovirus. Conclusion The RSV antigen-detecting method by ELISA can be applied to the clinical diagnose of the RSV infection.