中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2011年
5期
373-376
,共4页
李石峰%谭军%李高峰%黄泽春
李石峰%譚軍%李高峰%黃澤春
리석봉%담군%리고봉%황택춘
增生性瘢痕%强脉冲光%微血管密度%血管内皮细胞生长因子%增殖细胞核抗原
增生性瘢痕%彊脈遲光%微血管密度%血管內皮細胞生長因子%增殖細胞覈抗原
증생성반흔%강맥충광%미혈관밀도%혈관내피세포생장인자%증식세포핵항원
Hypertrophic scar%Intense pulsed light%Vascular endothelial growth factor%Proliferating cell nuclear antigen%Microvessel density
目的 探讨强脉冲光(intense pulsed light,IPL)对兔耳增生性瘢痕的作用及机制.方法 选取新西兰大白兔30只,在每只兔耳腹侧面制作2个增生性瘢痕模型,随机分为治疗组和对照组.治疗组瘢痕在建立模型第3、5、7周采用IPL进行治疗,对照组不进行治疗.观察各组瘢痕形态变化,并且于建立模型第3、5、7周IPL治疗前及建立模型第9周采集各组瘢痕组织标本,苏木精-伊红(HE)染色,采用免疫组织化学方法检测α平滑肌肌动蛋白(α-smooth mascle actin,α-SMA)、血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)、增殖细胞核抗原(proliferating cellnuclear antigen,PCNA)的表达,并利用α-SMA的表达进行微血管密度(microvessel density,MVD)计数,观察微血管变化.建立模型9周时,切取正常喂养的2只兔子的腹侧正常皮肤组织标本进行同上的检查.结果 经IPL治疗后的同期瘢痕色泽变淡、厚度降低.IPL治疗组瘢痕软化、完全平坦所需时间较对照组缩短.组织内α-SMA、VEGF和PCNA的表达均降低,差异有统计学意义(P＜0.05).结论 IPL对兔耳增生性瘢痕有明显的治疗作用.
目的 探討彊脈遲光(intense pulsed light,IPL)對兔耳增生性瘢痕的作用及機製.方法 選取新西蘭大白兔30隻,在每隻兔耳腹側麵製作2箇增生性瘢痕模型,隨機分為治療組和對照組.治療組瘢痕在建立模型第3、5、7週採用IPL進行治療,對照組不進行治療.觀察各組瘢痕形態變化,併且于建立模型第3、5、7週IPL治療前及建立模型第9週採集各組瘢痕組織標本,囌木精-伊紅(HE)染色,採用免疫組織化學方法檢測α平滑肌肌動蛋白(α-smooth mascle actin,α-SMA)、血管內皮細胞生長因子(vascular endothelial growth factor,VEGF)、增殖細胞覈抗原(proliferating cellnuclear antigen,PCNA)的錶達,併利用α-SMA的錶達進行微血管密度(microvessel density,MVD)計數,觀察微血管變化.建立模型9週時,切取正常餵養的2隻兔子的腹側正常皮膚組織標本進行同上的檢查.結果 經IPL治療後的同期瘢痕色澤變淡、厚度降低.IPL治療組瘢痕軟化、完全平坦所需時間較對照組縮短.組織內α-SMA、VEGF和PCNA的錶達均降低,差異有統計學意義(P＜0.05).結論 IPL對兔耳增生性瘢痕有明顯的治療作用.
목적 탐토강맥충광(intense pulsed light,IPL)대토이증생성반흔적작용급궤제.방법 선취신서란대백토30지,재매지토이복측면제작2개증생성반흔모형,수궤분위치료조화대조조.치료조반흔재건립모형제3、5、7주채용IPL진행치료,대조조불진행치료.관찰각조반흔형태변화,병차우건립모형제3、5、7주IPL치료전급건립모형제9주채집각조반흔조직표본,소목정-이홍(HE)염색,채용면역조직화학방법검측α평활기기동단백(α-smooth mascle actin,α-SMA)、혈관내피세포생장인자(vascular endothelial growth factor,VEGF)、증식세포핵항원(proliferating cellnuclear antigen,PCNA)적표체,병이용α-SMA적표체진행미혈관밀도(microvessel density,MVD)계수,관찰미혈관변화.건립모형9주시,절취정상위양적2지토자적복측정상피부조직표본진행동상적검사.결과 경IPL치료후적동기반흔색택변담、후도강저.IPL치료조반흔연화、완전평탄소수시간교대조조축단.조직내α-SMA、VEGF화PCNA적표체균강저,차이유통계학의의(P＜0.05).결론 IPL대토이증생성반흔유명현적치료작용.
Objective To explore the effect and mechanisms of intense pulsed light (IPL) in treating hypertrophic scars on rabbit ear.Methods Thirty New Zealand white rabbits were incruited in this study.Two hypertrophic scar models were made in the ventral surface of the rabbit ears with two lesions on each ear.The rabbits were divided randomly into two groups:the treatment group and the control group.Rabbits in the treatment groups were treated by IPL at the 3rd,5th,7th week after operation.Rabbits in the control groups were untreated.Morphological appearances of the hypertrophic scar were observed,and biopsies of scar were taken for HE stain and immunohistochemistry for the expression of vascular endothelial growth factor (VEGF),proliferating cell nuclear antigen (PCNA) and α-SMA,microvessel density was calculated by the expression of α-SMA at the 3rd,5th,7th,9th week after operation.At the 9th week after the operation,the ventral surface skin from two normally-fed rabbits were collected to undergo the same examination above.Results Comparing with the control group,the height of scars was reduced significantly in the IPL treatment group.The scars becaming soften and completely flat needed less time in the IPL treatment group.Comparing with the control group,the level of α-SMA,MVD,VEGF and PCNA expression in the treatment group obviously decreased over the same period (P＜0.05).Conclusions IPL is of great therapeutic effect on treating hypertrophic scar of rabbit ear.
