世界华人消化杂志
世界華人消化雜誌
세계화인소화잡지
WORLD CHINESE JOURNAL OF DIGESTOLOGY
2005年
16期
1974-1977
,共4页
薛秀兰%林菊生%孙雪梅%周鹤俊
薛秀蘭%林菊生%孫雪梅%週鶴俊
설수란%림국생%손설매%주학준
奥曲肽%钙%肝%星状细胞%大鼠%cAMP%cGMP
奧麯肽%鈣%肝%星狀細胞%大鼠%cAMP%cGMP
오곡태%개%간%성상세포%대서%cAMP%cGMP
目的:探讨在常氧和低氧条件下奥曲肽(octreotide)对大鼠肝星状细胞(HSC)胞内游离钙[Ca2+]i及增殖的调节.方法:采用钙荧光探针(Fura-2/AM)负载培养的大鼠HSC,观察常氧和低氧条件下培养48 h后octreotide 对HSC[Ca2+]i的调节,同时用四唑盐(MTT)比色法比较不同浓度的octreotide对大鼠HSC增殖的影响.环磷酸腺苷(cAMP)和环磷酸鸟苷(cGMP)放免分析药盒测cAMP,cGMP浓度.结果:与常氧条件相比,低氧条件下HSC[Ca2+]i显著升高(293.2±12.4 nmol/Lvs137.7±7.8 nmol/L,P＜0.01).500、800和1 000μg/L octreotide在常氧状态下可引起HSCs[Ca2+]i降低(P＜0.05),其值分别为92.5±2.5、83.8±2.3和76.6±2.2 nmol/L.低氧时500,800和1 000μg/Loctreotide可引起HSCs[Ca2+]i降低(P＜0.01),其值分别为204.3±7.4、174.1±4.8和156.6±6.6 nmol/L.常氧对照组A值(0.232±0.016)明显低于低氧对照组(0.533±0.036)(P＜0.01);经500、800和1 000μg/Loctreotide处理后,无论是常氧还是低氧状态下A值均明显降低(P＜0.01).低氧条件下cAMP和cGMP含量不发生改变(P＞0.05);无论是常氧还是低氧状态,经过octreotide 500μg/L处理后,cAMP和cGMP含量与相应对照组相比增高(cAMP:1.69±0.18 pmol/mgvs1.10±0.32pmol/mg,1.87±0.30 pmol/mgvs1.37±0.25 pmol/mg,P＜0.05;cGMP:1.08±0.24 pmol/mg vs 0.86±0.12pmol/mg,1.17±0.53 pmol/mg vs 0.89±0.20 pmol/mg,P＜0.05),而Octreotide 800,1 000μg/L组更高(cAMP:1.99±0.27,2.48±0.37 pmol/mg vs1.10±0.32 pmol/mg.P＜0.01;2.09±0.35,2.24±0.15 pmol/mg vs 1.37±0.25pmol/mg,P＜0.01;cGMP:1.24±0.17,1.31±0.29pmol/mg vs 0.86±0.12 pmol/mg,P＜0.01;1.38±0.29,1.46±0.35 pmol/mg vs 0.89±0.20 pmol/mg,P＜0.01).结论:缺氧可通过第二信使系统促进HSC的增生,而octreotide无论常氧还是低氧条件下剂量依赖性抑制HSCs增殖;cAMP,cGMP参与了对HSC的调节.
目的:探討在常氧和低氧條件下奧麯肽(octreotide)對大鼠肝星狀細胞(HSC)胞內遊離鈣[Ca2+]i及增殖的調節.方法:採用鈣熒光探針(Fura-2/AM)負載培養的大鼠HSC,觀察常氧和低氧條件下培養48 h後octreotide 對HSC[Ca2+]i的調節,同時用四唑鹽(MTT)比色法比較不同濃度的octreotide對大鼠HSC增殖的影響.環燐痠腺苷(cAMP)和環燐痠鳥苷(cGMP)放免分析藥盒測cAMP,cGMP濃度.結果:與常氧條件相比,低氧條件下HSC[Ca2+]i顯著升高(293.2±12.4 nmol/Lvs137.7±7.8 nmol/L,P＜0.01).500、800和1 000μg/L octreotide在常氧狀態下可引起HSCs[Ca2+]i降低(P＜0.05),其值分彆為92.5±2.5、83.8±2.3和76.6±2.2 nmol/L.低氧時500,800和1 000μg/Loctreotide可引起HSCs[Ca2+]i降低(P＜0.01),其值分彆為204.3±7.4、174.1±4.8和156.6±6.6 nmol/L.常氧對照組A值(0.232±0.016)明顯低于低氧對照組(0.533±0.036)(P＜0.01);經500、800和1 000μg/Loctreotide處理後,無論是常氧還是低氧狀態下A值均明顯降低(P＜0.01).低氧條件下cAMP和cGMP含量不髮生改變(P＞0.05);無論是常氧還是低氧狀態,經過octreotide 500μg/L處理後,cAMP和cGMP含量與相應對照組相比增高(cAMP:1.69±0.18 pmol/mgvs1.10±0.32pmol/mg,1.87±0.30 pmol/mgvs1.37±0.25 pmol/mg,P＜0.05;cGMP:1.08±0.24 pmol/mg vs 0.86±0.12pmol/mg,1.17±0.53 pmol/mg vs 0.89±0.20 pmol/mg,P＜0.05),而Octreotide 800,1 000μg/L組更高(cAMP:1.99±0.27,2.48±0.37 pmol/mg vs1.10±0.32 pmol/mg.P＜0.01;2.09±0.35,2.24±0.15 pmol/mg vs 1.37±0.25pmol/mg,P＜0.01;cGMP:1.24±0.17,1.31±0.29pmol/mg vs 0.86±0.12 pmol/mg,P＜0.01;1.38±0.29,1.46±0.35 pmol/mg vs 0.89±0.20 pmol/mg,P＜0.01).結論:缺氧可通過第二信使繫統促進HSC的增生,而octreotide無論常氧還是低氧條件下劑量依賴性抑製HSCs增殖;cAMP,cGMP參與瞭對HSC的調節.
목적:탐토재상양화저양조건하오곡태(octreotide)대대서간성상세포(HSC)포내유리개[Ca2+]i급증식적조절.방법:채용개형광탐침(Fura-2/AM)부재배양적대서HSC,관찰상양화저양조건하배양48 h후octreotide 대HSC[Ca2+]i적조절,동시용사서염(MTT)비색법비교불동농도적octreotide대대서HSC증식적영향.배린산선감(cAMP)화배린산조감(cGMP)방면분석약합측cAMP,cGMP농도.결과:여상양조건상비,저양조건하HSC[Ca2+]i현저승고(293.2±12.4 nmol/Lvs137.7±7.8 nmol/L,P＜0.01).500、800화1 000μg/L octreotide재상양상태하가인기HSCs[Ca2+]i강저(P＜0.05),기치분별위92.5±2.5、83.8±2.3화76.6±2.2 nmol/L.저양시500,800화1 000μg/Loctreotide가인기HSCs[Ca2+]i강저(P＜0.01),기치분별위204.3±7.4、174.1±4.8화156.6±6.6 nmol/L.상양대조조A치(0.232±0.016)명현저우저양대조조(0.533±0.036)(P＜0.01);경500、800화1 000μg/Loctreotide처리후,무론시상양환시저양상태하A치균명현강저(P＜0.01).저양조건하cAMP화cGMP함량불발생개변(P＞0.05);무론시상양환시저양상태,경과octreotide 500μg/L처리후,cAMP화cGMP함량여상응대조조상비증고(cAMP:1.69±0.18 pmol/mgvs1.10±0.32pmol/mg,1.87±0.30 pmol/mgvs1.37±0.25 pmol/mg,P＜0.05;cGMP:1.08±0.24 pmol/mg vs 0.86±0.12pmol/mg,1.17±0.53 pmol/mg vs 0.89±0.20 pmol/mg,P＜0.05),이Octreotide 800,1 000μg/L조경고(cAMP:1.99±0.27,2.48±0.37 pmol/mg vs1.10±0.32 pmol/mg.P＜0.01;2.09±0.35,2.24±0.15 pmol/mg vs 1.37±0.25pmol/mg,P＜0.01;cGMP:1.24±0.17,1.31±0.29pmol/mg vs 0.86±0.12 pmol/mg,P＜0.01;1.38±0.29,1.46±0.35 pmol/mg vs 0.89±0.20 pmol/mg,P＜0.01).결론:결양가통과제이신사계통촉진HSC적증생,이octreotide무론상양환시저양조건하제량의뢰성억제HSCs증식;cAMP,cGMP삼여료대HSC적조절.
