背景:脑醒喷鼻剂由川芎和石菖蒲等中药组成,<神农本草经>谓其"主脑卒中入脑"之功效.目的:观察脑醒喷鼻剂对大鼠局灶性脑缺血再灌注损伤脑组织自由基及一氧化氮合酶变化的影响,并与经典西药尼莫地平相比较.设计:随机对照实验.单位:一所中医药大学医院的内科.对象:清洁级成年雄性Wistar大鼠70只,随机分为7组:脑醒喷鼻剂高剂量组(高剂组),脑醒喷鼻剂中剂量组(中剂组),脑醒喷鼻剂低剂量组(低剂组),尼莫地平腹腔注射组(尼莫地平组),生理盐水喷鼻剂组(生理盐水组),溶酶喷鼻剂组(溶酶组),假手术组,每组10只.方法:除假手术组外,其他6组阻断大鼠左侧大脑中动脉建立大鼠局灶脑缺血再灌注模型.在造模前3 d及再灌注期间,脑醒喷鼻剂高、中、低剂组分别以含川芎嗪和石菖蒲生药5.4,2.7,1.08 mg/(kg·d)和1 35,0.54,0.27g/(kg·d)的剂量滴鼻,3次/d;生理盐水组和溶酶组以生理盐水、溶酶喷鼻剂0.18 mL/(kg·d)滴鼻,3次/d;尼莫地平组用尼莫地平注射液0.8 mg/(kg·d)腹腔注射,2次/d;假手术组按常规饲养至实验取材.用比色法检测丙二醛、超氧化物歧化酶及一氧化氮合酶.主要观察指标:①不同剂量脑醒喷鼻剂及其他组动物脑组织丙二醛、超氧化物歧化酶及一氧化氮合酶活性变化.②将不同剂量脑醒喷鼻剂组与尼莫地平组做比较.结果:造模时死亡8只动物,进入结果分析62只.①丙二醛含量:高剂组、尼莫地平组明显低于生理盐水组[(0.92±0.32),(0.87±0.39),(1.35±0.34)μmol/g,P＜0.05],但高剂组和尼莫地平组间无差异.②超氧化物歧化酶活性:高剂组、尼莫地平组明显高于生理盐水组[(35.64±11.67),(33.88±7.15),(20.70±3.88)NU/mg,P＜0.05],但高剂组和尼莫地平组间无差异.③一氧化氮合酶活性及超氧化物歧化酶活性:高剂组、尼莫地平组明显高于生理盐水组[(4.64±1.22),(5.00±1.10),(3.08±1.12)mkat/g,P＜0.05],但高剂组和尼莫地平组间无差异.④中、低剂组和溶酶组超氧化物歧化酶及一氧化氮合酶活性有所升高,丙二醛含量有所降低,但与生理盐水组比较无差异.结论:脑醒喷鼻剂高剂量组能防止脑缺血缺氧所致的脂质过氧化,使丙二醛生成减少,清除自由基损害,并增加一氧化氮合酶活性,对脑组织的缺血再灌注损伤有保护作用,与尼莫地平的作用相当.
揹景:腦醒噴鼻劑由川芎和石菖蒲等中藥組成,<神農本草經>謂其"主腦卒中入腦"之功效.目的:觀察腦醒噴鼻劑對大鼠跼竈性腦缺血再灌註損傷腦組織自由基及一氧化氮閤酶變化的影響,併與經典西藥尼莫地平相比較.設計:隨機對照實驗.單位:一所中醫藥大學醫院的內科.對象:清潔級成年雄性Wistar大鼠70隻,隨機分為7組:腦醒噴鼻劑高劑量組(高劑組),腦醒噴鼻劑中劑量組(中劑組),腦醒噴鼻劑低劑量組(低劑組),尼莫地平腹腔註射組(尼莫地平組),生理鹽水噴鼻劑組(生理鹽水組),溶酶噴鼻劑組(溶酶組),假手術組,每組10隻.方法:除假手術組外,其他6組阻斷大鼠左側大腦中動脈建立大鼠跼竈腦缺血再灌註模型.在造模前3 d及再灌註期間,腦醒噴鼻劑高、中、低劑組分彆以含川芎嗪和石菖蒲生藥5.4,2.7,1.08 mg/(kg·d)和1 35,0.54,0.27g/(kg·d)的劑量滴鼻,3次/d;生理鹽水組和溶酶組以生理鹽水、溶酶噴鼻劑0.18 mL/(kg·d)滴鼻,3次/d;尼莫地平組用尼莫地平註射液0.8 mg/(kg·d)腹腔註射,2次/d;假手術組按常規飼養至實驗取材.用比色法檢測丙二醛、超氧化物歧化酶及一氧化氮閤酶.主要觀察指標:①不同劑量腦醒噴鼻劑及其他組動物腦組織丙二醛、超氧化物歧化酶及一氧化氮閤酶活性變化.②將不同劑量腦醒噴鼻劑組與尼莫地平組做比較.結果:造模時死亡8隻動物,進入結果分析62隻.①丙二醛含量:高劑組、尼莫地平組明顯低于生理鹽水組[(0.92±0.32),(0.87±0.39),(1.35±0.34)μmol/g,P＜0.05],但高劑組和尼莫地平組間無差異.②超氧化物歧化酶活性:高劑組、尼莫地平組明顯高于生理鹽水組[(35.64±11.67),(33.88±7.15),(20.70±3.88)NU/mg,P＜0.05],但高劑組和尼莫地平組間無差異.③一氧化氮閤酶活性及超氧化物歧化酶活性:高劑組、尼莫地平組明顯高于生理鹽水組[(4.64±1.22),(5.00±1.10),(3.08±1.12)mkat/g,P＜0.05],但高劑組和尼莫地平組間無差異.④中、低劑組和溶酶組超氧化物歧化酶及一氧化氮閤酶活性有所升高,丙二醛含量有所降低,但與生理鹽水組比較無差異.結論:腦醒噴鼻劑高劑量組能防止腦缺血缺氧所緻的脂質過氧化,使丙二醛生成減少,清除自由基損害,併增加一氧化氮閤酶活性,對腦組織的缺血再灌註損傷有保護作用,與尼莫地平的作用相噹.
배경:뇌성분비제유천궁화석창포등중약조성,<신농본초경>위기"주뇌졸중입뇌"지공효.목적:관찰뇌성분비제대대서국조성뇌결혈재관주손상뇌조직자유기급일양화담합매변화적영향,병여경전서약니막지평상비교.설계:수궤대조실험.단위:일소중의약대학의원적내과.대상:청길급성년웅성Wistar대서70지,수궤분위7조:뇌성분비제고제량조(고제조),뇌성분비제중제량조(중제조),뇌성분비제저제량조(저제조),니막지평복강주사조(니막지평조),생리염수분비제조(생리염수조),용매분비제조(용매조),가수술조,매조10지.방법:제가수술조외,기타6조조단대서좌측대뇌중동맥건립대서국조뇌결혈재관주모형.재조모전3 d급재관주기간,뇌성분비제고、중、저제조분별이함천궁진화석창포생약5.4,2.7,1.08 mg/(kg·d)화1 35,0.54,0.27g/(kg·d)적제량적비,3차/d;생리염수조화용매조이생리염수、용매분비제0.18 mL/(kg·d)적비,3차/d;니막지평조용니막지평주사액0.8 mg/(kg·d)복강주사,2차/d;가수술조안상규사양지실험취재.용비색법검측병이철、초양화물기화매급일양화담합매.주요관찰지표:①불동제량뇌성분비제급기타조동물뇌조직병이철、초양화물기화매급일양화담합매활성변화.②장불동제량뇌성분비제조여니막지평조주비교.결과:조모시사망8지동물,진입결과분석62지.①병이철함량:고제조、니막지평조명현저우생리염수조[(0.92±0.32),(0.87±0.39),(1.35±0.34)μmol/g,P＜0.05],단고제조화니막지평조간무차이.②초양화물기화매활성:고제조、니막지평조명현고우생리염수조[(35.64±11.67),(33.88±7.15),(20.70±3.88)NU/mg,P＜0.05],단고제조화니막지평조간무차이.③일양화담합매활성급초양화물기화매활성:고제조、니막지평조명현고우생리염수조[(4.64±1.22),(5.00±1.10),(3.08±1.12)mkat/g,P＜0.05],단고제조화니막지평조간무차이.④중、저제조화용매조초양화물기화매급일양화담합매활성유소승고,병이철함량유소강저,단여생리염수조비교무차이.결론:뇌성분비제고제량조능방지뇌결혈결양소치적지질과양화,사병이철생성감소,청제자유기손해,병증가일양화담합매활성,대뇌조직적결혈재관주손상유보호작용,여니막지평적작용상당.
BACKGROUND: Brain-awakening nasal sprayer is composed of many herbs,such as Chuanxiong and Shichangpu, which were regarded by "Shennong Bencaojing" as having the function of "preventing stroke in the brain".OBJECTIVE: To observe the changes of free radicals and nitric oxide synthase in rat brain following focal ischemic-reperfusional injury due to brain-awakening nasal sprayer intervention and compare with that due to classical nimodipine.DESIGN: A randomized controlled study.SETTING: Department of internal medicine of a hospital affiliated to a traditional Chinese medical university.MATERIALS: Seventy adult male Wistar rats of clean grade, were randomly divided into seven groups: brain-awakening nasal sprayer of higher dosage group, moderate dosage group, lower dosage group, nimodipine intraperitoneal injection group, physical saline nasal sprayer group, menstruum nasal sprayer group, and sham operation group with 10 rats in each.METHODS: Focal brain ischemia-reperfusion model was established by blocking the left cerebral middle artery in rats of all the groups except sham operation group. Three days before model establishment and during reperfusion, rats were given different dosages of brain-awakening nasal sprayer composed of Chuanxiongqin and Shichangpu of 5.4, 2.7, 1.08 mg/(kg · d) and 1.35, 0. 54, 0.27 g/(kg· d), respectively, three times a day; which was replaced by physical saline and menstruum nasal sprayer of 0. 18 mL/ (kg · d),three times a day in physical saline nasal sprayer group and menstruum nasal sprayer group; rats in nimodipine intraperitoneal injection group received intraperitoneal injection of nimodipine by 0. 8 mg/(kg · d) twice a day. Rats in sham operation group were routinely raised. The content of prodialdehyde, superoxide dismutase and nitric oxide synthase were measured with colorimetric method.MAIN OUTCOME MEASURES: ① The changes of prodialdehyde content, superoxide dismutase and nitric oxide synthase activity in rat brain following focal ischemic-reperfusional injury in groups of different dosage of brain-awakening nasal sprayer and other groups. ② Comparison between different dosage brain-awakening nasal sprayer intervention groups and nimodipine intraperitoneal injection group.RESULTS: Eight rats died during model establishment and the other 62 rats entered the results analysis. ① Content of prodialdehyde: It was significantly lower in higher dosage group and nimodipine intraperitoneal injection group than in physical saline nasal sprayer group [ (0.92 ± 0. 32), (0. 87 ± 0. 39)vs(1.35 ±0. 34) μmol/g, P ＜ 0.05], but there was no difference between higher dosage group and nimodipine intraperitoneal injection group. ② Activity of superoxide dismutase: It was obviously higher in higher dosage group and nimodipine intraperitoneal injection group than in physical saline nasal sprayer group[ (35.64 ± 11.67), (33.88 ± 7. 15) vs(20. 70 ± 3.88) NU/mg,P ＜ 0. 05 ], but no difference could be observed between higher dosage group and nimodipine intraperitoneal injection group. ③ Activity of nitric oxide synthase and superoxide dismutase: It was found obviously higher in higher dosage group and nimodipine intraperitoneal injection group than in physical saline nasal sprayer group[ (4.64 ± 1.22), (5.00 ± 1.10) vs (3.08 ± 1.12) mkat/g, P ＜ 0.05], but no difference could be observed between higher dosage group and nimodipine intraperitoneal injection group.④ The activity of nitric oxide synthase and superoxide dismutase slightly increased while prodialdehyde slightly decreased in moderate dosage group,lower dosage group and menstruum nasal sprayer group, which did not differ significantly from physical saline nasal sprayer group.CONCLUSION: Brain-awakening nasal sprayer intervention exerts multiple effects such as preventing lipo-peroxidation following brain ischemic- reperfusional injury, in addition to suppressing prodialdehyde production, attenuating injury induced by free radicals and increasing nitric oxide synthase activity, thereby playing a similar role to nimodipine in protecting against brain ischemic-reperfusional damage
