CAJ | 학술논문

目的应用液基薄层细胞学技术筛查女性宫颈癌,并对部分患者检测人乳头状瘤病毒(HPV)-16、-18型.方法采用液基薄层细胞学技术对医院妇科门诊患者及妇科体检者651例进行宫颈癌筛查,并采用荧光定量PCR对28例细胞学阴性者和13例低级别病变以上患者检测HPV-16、-18型.结果 651例样本检出低级别病变11例,高级别病变2例,检出率分别为1.69%和0.31%,总检出率为2.0%;28例细胞学阴性者检出HPV-16、-18型3例,感染率为7.14%;13例低级别以上患者检出HPV-16、-18型6例,感染率为46.15%,感染率差异具有统计学意义.结论液基薄层细胞学技术简便实用,适宜于女性宫颈癌筛查;联合HPV-16、-18型检测更有意义.
목적 응용액기박층세포학기술사사녀성궁경암,병대부분환자검측인유두상류병독(HPV)-16、-18형.방법 채용액기박층세포학기술대의원부과문진환자급부과체검자651례진행궁경암사사,병채용형광정량PCR대28례세포학음성자화13례저급별병변이상환자검측HPV-16、-18형.결과 651례양본검출저급별병변11례,고급별병변2례,검출솔분별위1.69%화0.31%,총검출솔위2.0%;28례세포학음성자검출HPV-16、-18형3례,감염솔위7.14%;13례저급별이상환자검출HPV-16、-18형6례,감염솔위46.15%,감염솔차이구유통계학의의.결론 액기박층세포학기술간편실용,괄의우녀성궁경암사사;연합HPV-16、-18형검측경유의의.
Objective To screen cervical cancer by thinprep cytology test (TCT) and detect human papilloma virus (HPV)-16, -18 in some patients.Methods TCT was applied to screening cervical cancer in 651 cases of gynecology outpatients and healthy examination people, while real-time fluorescence quantitive PCR was adopted to detect HPV-16 and -18 in 28 cases of subjects with normal cytology and 13 cases of patients with low grade or above lesion.Results In 651 cases of samples, 11 cases (1.69%) of low grade and 2 cases (0.31%) of high grade squamous intraepithelial lesion were detected with TCT, and the total detection rate was 2.0%. The detected cases (%) of HPV-16 and -18 were 3 (7.14%) in 28 cases of cytology negative women, and 6 (46.15%) in 13 patients with low grade or above lesion. The difference of infection rate was statistically significant.Conclusion Thinprep cytology test is simple and practical, suitable for screening of cervical cancer. It is more significant to detect HPV-16 and -18 simultaneously.