CAJ | 학술논문

目的观察拉米夫定(LAM)治疗前后患者血清Th1/Th2比值的动态变化.方法用酶联免疫吸附(ELISA)方法分别检测60例慢性乙型肝炎患者在拉米夫定治疗前,治疗后第3、6、9、12月时的血清IFN-γ和IL-4水平.选取20名健康献血员作为正常对照.结果 LAM治疗前ALT高水平组IFN-γ水平及IFN-γ/IL-4比值较高,完全应答率较高,无应答率较低;治疗后完全应答组IFN-γ/IL-4水平接近或高于对照组,部分应答组和无应答组IFN-γ/IL-4水平低于对照组.结论拉米夫定治疗慢性乙型肝炎可增加Th1类细胞因子IFN-γ分泌,抑制Th2类细胞因子IL-4分泌,治疗后Th1/Th2平衡的恢复与抗病毒疗效有关.T细胞免疫功能的恢复是抗病毒治疗的关键之一.
목적 관찰랍미부정(LAM)치료전후환자혈청Th1/Th2비치적동태변화.방법 용매련면역흡부(ELISA)방법분별검측60례만성을형간염환자재랍미부정치료전,치료후제3、6、9、12월시적혈청IFN-γ화IL-4수평.선취20명건강헌혈원작위정상대조.결과 LAM치료전ALT고수평조IFN-γ수평급IFN-γ/IL-4비치교고,완전응답솔교고,무응답솔교저;치료후완전응답조IFN-γ/IL-4수평접근혹고우대조조,부분응답조화무응답조IFN-γ/IL-4수평저우대조조.결론 랍미부정치료만성을형간염가증가Th1류세포인자IFN-γ분비,억제Th2류세포인자IL-4분비,치료후Th1/Th2평형적회복여항병독료효유관.T세포면역공능적회복시항병독치료적관건지일.
Objective To investigate changes in serum Th1/Th2 ratios of patients with chronic hepatitis B during Lamivudine treatment.Methods Serum INF-γ and IL-4 levels were determined by ELISA in 60 patients with chronic hepatitis B at baseline and 3, 6, 9 and 12 months during treatment. At the same time, and 20 healthy volunteers were served as normal control.Results The patients with high ALT levels showed higher IFN-γ levels and IFN-γ/IL-4 ratios than those with low ALT levels,and they showed higher rate of complete response and lower rate of non-response to LAM treatment. After treatment, the ratios of IFN-γ to IL-4 in complete response patients were approximate to those of control group, sometimes even higher than them. The ratios of IFN-γ to IL-4 in partial response and non response patients were lower than those of control group.Conclusion LAM can increase the release of IFN-γ(cytokines of Th1 type ), whereas inhibit IL-4(cytokines of Th2 type ) levels. The antiviral effect is related to the recovery of Th1/Th2 balance. The reconstruction of the T lymphocyte function in vivo is an important aspect in antiviral treatment.