CAJ | 학술논문

目的探讨含糖腹透液对大鼠腹膜间皮细胞功能的影响.方法体外培养的大鼠腹膜间皮细胞,用流式细胞仪检测细胞凋亡率,同时观察细胞内游离钙的水平和ICAM-1表达水平.结果腹膜透析液刺激1 h后,体外培养的大鼠腹膜间皮细胞早期凋亡率增高;随着腹透液中糖浓度越高,刺激时间越长,凋亡率增高越明显,ICAM-1表达显著增加,细胞内游离钙水平下降.结论高糖腹透液可以增加腹膜间皮细胞其凋亡率,降低细胞游离钙水平和增加ICAM-1的表达,这些改变可能是长期腹透病人腹膜纤维化和发生失超滤的原因.
목적 탐토함당복투액대대서복막간피세포공능적영향.방법 체외배양적대서복막간피세포,용류식세포의검측세포조망솔,동시관찰세포내유리개적수평화ICAM-1표체수평.결과 복막투석액자격1 h후,체외배양적대서복막간피세포조기조망솔증고;수착복투액중당농도월고,자격시간월장,조망솔증고월명현,ICAM-1표체현저증가,세포내유리개수평하강.결론 고당복투액가이증가복막간피세포기조망솔,강저세포유리개수평화증가ICAM-1적표체,저사개변가능시장기복투병인복막섬유화화발생실초려적원인.
Objective To investigate the effects of peritoneal dialysis solution (PDS) on apoptosis and intracellular free calcium ([Ca~(2+)]i), cell surface ICAM-1 expression of rat peritoneal mesothelial cells (RPMCs). Methods The RPMCs apoptosis rate were determined by flow cytometry. [Ca~(2+)]i in the cells were monitered the fluorescence at 528 nm by confocus laser microscopy. Cell surface ICAM-1 expression were detected by flow cytometry. Results After PDS treatment for 1 h, the RPMCs apoptosis rate were increased. Such increase was more manifest with higher glucose concentration in PDS and longer treatment time of the cells. At the same times, after 3 hours, ICAM-1 expressions of the PDS containing glucose and mannitol are all increased. With the increase of glucose concentrations, the descend of [Ca~(2+)]i levels were aggravated.Conclusion PDS containing high- concentration glucose can induce significant apoptosis of RPMCs in vitro. This may be related with the enhanced level of ICAM-1 expressions and the decreased level of [Ca~(2+)]i. Which may due to the occurrence of peritoneal fibrosis and ultrafiltrate failure in patients suffering long term peritoneal dialysis.