中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2010年
1期
22-25
,共4页
连方%赵斌%吕雪梅%张建伟%孙振高%张宁%王利红%刘延荷%马凤梅%牟善芳%孟茜
連方%趙斌%呂雪梅%張建偉%孫振高%張寧%王利紅%劉延荷%馬鳳梅%牟善芳%孟茜
련방%조빈%려설매%장건위%손진고%장저%왕리홍%류연하%마봉매%모선방%맹천
二至天癸颗粒%体外受精-胚胎移植%代谢组学%钙离子%卵细胞质量
二至天癸顆粒%體外受精-胚胎移植%代謝組學%鈣離子%卵細胞質量
이지천계과립%체외수정-배태이식%대사조학%개리자%란세포질량
Er'zhi Tiangui Granule%in vitro ferbilization and embryo transfer%metabonomics%calcium ion%quality of oocyte ion%quality of oocyte
目的 从人卵泡液代谢组学及Ca~(2+)浓度,探讨二至天癸颗粒提高卵细胞质量的机制.探讨体外受精-胚胎移植(in vitro fertilization and embryo transfer,IVF-ET)周期人卵泡液代谢组学及钙离子(Ca~(2+))浓度、胆碱脂酶(ChE)、肌酐(Cr)对卵细胞质量及妊娠结局的影响.方法 57例IVF-ET患者随机分为试验组(二至天癸颗粒加IVF-ET常规用药组,27例)和对照组(六味地黄颗粒合IVF-ET常规用药组,30例).观察患者取卵日肾阴虚证候较垂体降调节后的改善情况,比较两组患者卵泡液Ca~(2+)浓度、胆碱脂酶、肌酐的差异及两组卵泡液代谢组学的变化,取卵数、受精率、卵裂率、优质胚胎率和妊娠率的差异.结果 试验组治疗后肾阴虚证候积分为8.30±1.46,受精率0.82±0.09,卵裂率0.97±0.07,优质胚胎率0.51±0.18,对照组分别为9.16±1.15,0.74±0.18,0.91±0.10,0.41±0.09,试验组优于对照组(P<0.05).主成分分析法分析两组卵泡液代谢组学,试验组主要分布在Ⅰ区;对照组主要分布在Ⅱ区.两组卵泡液代谢组学有明显差异.妊娠组卵泡液主要分布在Ⅰ区.试验组Ca~(2+)浓度显著高于对照组(P<0.05).ChE、Cr高于对照组,但差异无统计学意义.结论 在调节卵泡液代谢组学和Ca~(2+)浓度,提高卵细胞、胚胎质量,提高IVF-ET妊娠率方面,二至天癸颗粒优于六味地黄颗粒.
目的 從人卵泡液代謝組學及Ca~(2+)濃度,探討二至天癸顆粒提高卵細胞質量的機製.探討體外受精-胚胎移植(in vitro fertilization and embryo transfer,IVF-ET)週期人卵泡液代謝組學及鈣離子(Ca~(2+))濃度、膽堿脂酶(ChE)、肌酐(Cr)對卵細胞質量及妊娠結跼的影響.方法 57例IVF-ET患者隨機分為試驗組(二至天癸顆粒加IVF-ET常規用藥組,27例)和對照組(六味地黃顆粒閤IVF-ET常規用藥組,30例).觀察患者取卵日腎陰虛證候較垂體降調節後的改善情況,比較兩組患者卵泡液Ca~(2+)濃度、膽堿脂酶、肌酐的差異及兩組卵泡液代謝組學的變化,取卵數、受精率、卵裂率、優質胚胎率和妊娠率的差異.結果 試驗組治療後腎陰虛證候積分為8.30±1.46,受精率0.82±0.09,卵裂率0.97±0.07,優質胚胎率0.51±0.18,對照組分彆為9.16±1.15,0.74±0.18,0.91±0.10,0.41±0.09,試驗組優于對照組(P<0.05).主成分分析法分析兩組卵泡液代謝組學,試驗組主要分佈在Ⅰ區;對照組主要分佈在Ⅱ區.兩組卵泡液代謝組學有明顯差異.妊娠組卵泡液主要分佈在Ⅰ區.試驗組Ca~(2+)濃度顯著高于對照組(P<0.05).ChE、Cr高于對照組,但差異無統計學意義.結論 在調節卵泡液代謝組學和Ca~(2+)濃度,提高卵細胞、胚胎質量,提高IVF-ET妊娠率方麵,二至天癸顆粒優于六味地黃顆粒.
목적 종인란포액대사조학급Ca~(2+)농도,탐토이지천계과립제고란세포질량적궤제.탐토체외수정-배태이식(in vitro fertilization and embryo transfer,IVF-ET)주기인란포액대사조학급개리자(Ca~(2+))농도、담감지매(ChE)、기항(Cr)대란세포질량급임신결국적영향.방법 57례IVF-ET환자수궤분위시험조(이지천계과립가IVF-ET상규용약조,27례)화대조조(륙미지황과립합IVF-ET상규용약조,30례).관찰환자취란일신음허증후교수체강조절후적개선정황,비교량조환자란포액Ca~(2+)농도、담감지매、기항적차이급량조란포액대사조학적변화,취란수、수정솔、란렬솔、우질배태솔화임신솔적차이.결과 시험조치료후신음허증후적분위8.30±1.46,수정솔0.82±0.09,란렬솔0.97±0.07,우질배태솔0.51±0.18,대조조분별위9.16±1.15,0.74±0.18,0.91±0.10,0.41±0.09,시험조우우대조조(P<0.05).주성분분석법분석량조란포액대사조학,시험조주요분포재Ⅰ구;대조조주요분포재Ⅱ구.량조란포액대사조학유명현차이.임신조란포액주요분포재Ⅰ구.시험조Ca~(2+)농도현저고우대조조(P<0.05).ChE、Cr고우대조조,단차이무통계학의의.결론 재조절란포액대사조학화Ca~(2+)농도,제고란세포、배태질량,제고IVF-ET임신솔방면,이지천계과립우우륙미지황과립.
Objective To explore the mechanism of action of Er'zhi Tiangui Granule (ETG) in improving quality of oocytes by observing metabonomics and level of calcium ion in follicle fluid, and to investigate the impacts of calcium ion, cholinesterase (ChE) and creatinine (CCr) levels in human follicle fluid on the quality of oocytes and outcome of pregnancy in patients after in vitro fertilization and embryo transfer (IVF-ET). Methods Fifty-seven patients after IVF-ET were randomly assigned to two groups: the trial group (27 patients) and the control group (30 patients), both were treated with conventional Western medical treatment, but ETG and Liuwei Dihuang Granule (LDG) was given respectively to the two groups additionally. Changes of Shen-asthenia syndrome, amount of oocyte obtained, fertilization rate, cleavage rate, high-quality embryo rate and pregnancy rate, levels of calcium ion, ChE and Cr in follicle fluid, and metabonomics in the two groups were observed and compared. Results After treatment, scores of Shen-asthenia syndrome 8.30±1.46, fertilization rate 0.82±0.09, cleavage rate 0.97±0.07 and high-quality embryos rate 0.51±0.18 in the trial group were all better than those in the control group(9.16±1.15, 0.74±0.18, 0.91±0.10, 0.41±0.09, respectively, P<0.05); metabonomics principal component analysis showed that in the trial group, the principal component in follicle fluid distributed mainly in the section Ⅰ, and that in the control group distributed mainly in the section Ⅱ, showing significant difference between the two groups; while in most pregnant patients, it distributed in the section Ⅰ. The Ca~(2+) concentration in the trial group was significantly higher than that in the control group (P<0.05). Levels of ChE and Cr in the trial group were higher than those in the control group, but the difference between them was insignificant. Conclusion ETG is better than LDG in regulating metabonomics, Ca~(2+) concentration, improving the quality of oocyte and embryo, and increasing pregnant rate in patients after IVF-ET.
