CAJ | 학술논문

目的探讨CD55和CD59在健康者的红细胞及中性粒细胞、单核细胞、淋巴细胞上的表达.方法采用流式细胞技术检测健康体检者外周血红细胞及中性粒细胞、单核细胞、淋巴细胞膜上CD55和CD59表达的百分率及平均荧光强度.结果 CD55在中性粒细胞及单核细胞上的表达率(99.50±1.987,98.63±1.629)及平均荧光强度(11.14±2.927,13.37±4.127)均高于在淋巴细胞和红细胞上的表达(P＜0.01);CD59在红细胞、中性粒细胞及单核细胞上均高表达(大于98.21±1.857)且差异无统计学意义(P＞0.05),在淋巴细胞上的表达及平均荧光强度均低于其他组(P＜0.01),CD59在红细胞和中性粒细胞上表达的荧光强度(8.65±1.655,8.595±2.091)高于其他两组(P＜0.01).结论采用流式细胞术检测CD55、CD59表达时应选取中性粒细胞及成熟红细胞作为靶细胞.
목적 탐토CD55화CD59재건강자적홍세포급중성립세포、단핵세포、림파세포상적표체.방법 채용류식세포기술검측건강체검자외주혈홍세포급중성립세포、단핵세포、림파세포막상CD55화CD59표체적백분솔급평균형광강도.결과 CD55재중성립세포급단핵세포상적표체솔(99.50±1.987,98.63±1.629)급평균형광강도(11.14±2.927,13.37±4.127)균고우재림파세포화홍세포상적표체(P＜0.01);CD59재홍세포、중성립세포급단핵세포상균고표체(대우98.21±1.857)차차이무통계학의의(P＞0.05),재림파세포상적표체급평균형광강도균저우기타조(P＜0.01),CD59재홍세포화중성립세포상표체적형광강도(8.65±1.655,8.595±2.091)고우기타량조(P＜0.01).결론 채용류식세포술검측CD55、CD59표체시응선취중성립세포급성숙홍세포작위파세포.
Objective To investigate the expression of CD55 and CD59 on erythrocytes,neutrophils,monocytes and lymphocytes of healthy population.Methods The expression of CD55 and CD59 on red blood cells and different leukocytes of healthy persons were detected with flow eytometer.Results The expression of CD55 on neutrophils and monocytes were higher than that on lymphocytes and erythrocytes(P＜0.01).And there were no significant differences among the expressions of CD59 on erythrocytes,neutrophils and monocytes(P＞0.05).But the expression of CD59 on lymphocytes were lower that that on other cells(P＜0.01).Conclusion Neutrophil and erythrocyte is suitable of analysis when CD55 and CD59 are detected.