CAJ | 학술논문

目的分析肺栓塞(PE)患者外周血单个核细胞IFN相关基因mRNA表达.方法采用全人类基因组表达谱芯片检测PE组(20例PE患者)和对照组(20例对照者)受试者外周血单个核细胞( PBMC) IFN相关基因mRNA表达.结果与对照组比,PE组受试者PBMC I型IFN中IFNα5mRNA、IFNα6 mRNA、IFNα8mRNA、IFNα14 mRNA、IFNκmRNA、IFNω1 mRNA、IFNε1 mRNA表达下调,差异有统计学意义(P＜0.05)；Ⅰ型IFN受体IFNαR1mRNA、IFNαR2 mRNA表达差异无统计学意义(P＞0.05)；Ⅱ型IFN-IFNγmRNA表达下调,差异有统计学意义(P＜0.05)；IFNγ受体——IFNγ R1 mRNA、IFNγR2mRNA表达上调,差异有统计学意义(P＜0.05).结论 PE患者中Ⅰ型和Ⅱ型IFN基因表达显著下调,提示IFN下降是分泌生成减少,结果导致PE患者对病毒、胞内菌、寄生虫易感.免疫功能降低在PE的病理生理中发挥重要作用.
목적 분석폐전새(PE)환자외주혈단개핵세포IFN상관기인mRNA표체.방법 채용전인류기인조표체보심편검측PE조(20례PE환자)화대조조(20례대조자)수시자외주혈단개핵세포( PBMC) IFN상관기인mRNA표체.결과 여대조조비,PE조수시자PBMC I형IFN중IFNα5mRNA、IFNα6 mRNA、IFNα8mRNA、IFNα14 mRNA、IFNκmRNA、IFNω1 mRNA、IFNε1 mRNA표체하조,차이유통계학의의(P＜0.05)；Ⅰ형IFN수체IFNαR1mRNA、IFNαR2 mRNA표체차이무통계학의의(P＞0.05)；Ⅱ형IFN-IFNγmRNA표체하조,차이유통계학의의(P＜0.05)；IFNγ수체——IFNγ R1 mRNA、IFNγR2mRNA표체상조,차이유통계학의의(P＜0.05).결론 PE환자중Ⅰ형화Ⅱ형IFN기인표체현저하조,제시IFN하강시분비생성감소,결과도치PE환자대병독、포내균、기생충역감.면역공능강저재PE적병리생리중발휘중요작용.
Objective To investigate the gene expression difference of IFN and their receptors in peripheral blood mononuclear cells (PBMC) of pulmonary embolism (PE) patients.Methods Twenty cases of PE patients and twenty sex and age matched controls were recruited into the study.Human cDNA microarray analysis was used to detect the gene expression difference of IFN associated genes between the two groups,and random variance model corrected t test was used to analyze the statistical data.Results In comparison with the control group, mRNA expression of type Ⅰ IFN, including IFNα5 mRNA,IFNα6 mRNA,IFNα8 mRNA,IFNα14 mRNA,IFNκ mRNA,IFNω1 mRNA,IFNε1 mRNA in PBMC of PE patients Were down-regulated (P ＜ 0.05 ).There was no significant difference in gene expression of type Ⅰ IFN receptors IFNαR1 and IFNαR2 between the PE and control groups (P ＞ 0.05 ).In comparison with the control group,mRNA expression of IFNγ gene was down-regulated ( P ＜ 0.05 ).The mRNA expression of IFNγR1 and IFNγR2 genes were upregulated compared with the control (P ＞ 0.05 ).Conclusion mRNA expression of type Ⅰ and type Ⅱ IFN in PE are significantly down-regulated,but not the IFN receptors.Reduced immune function may play an important role in the PE patients who are susceptible to virus,intracellular bacteria and parasites.